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OfflineBackbone
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Are all the spores from a clone the same?
    #26473841 - 02/07/20 12:04 PM (4 years, 10 days ago)

spores?:thumbup:


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OfflineGrowtech
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Re: Are all the spores from a clone the same? [Re: Backbone]
    #26473846 - 02/07/20 12:07 PM (4 years, 10 days ago)

No... spores are genetically different.  It would be like if an identical twin human could only produce clones of themselves when they have kids (also not the case).


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OfflineNichrome
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Re: Are all the spores from a clone the same? [Re: Growtech] * 1
    #26473855 - 02/07/20 12:15 PM (4 years, 10 days ago)

All mushrooms are more than one organism. Spores are sexual reproduction.


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OfflineBackbone
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Re: Are all the spores from a clone the same? [Re: Nichrome]
    #26473872 - 02/07/20 12:24 PM (4 years, 10 days ago)

spores arent reproduction, two spores are needed to germinate


Edited by Backbone (02/07/20 12:28 PM)


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OfflineBrian Jones
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Re: Are all the spores from a clone the same? [Re: Backbone]
    #26473897 - 02/07/20 12:41 PM (4 years, 10 days ago)

I believe (correct me if I'm wrong) that even the tissue used to make a clone is made up of multiple genetics. That is why you have to make many transfers, and pay attention to sectoring, to get to an isolate.


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OfflineBackbone
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Re: Are all the spores from a clone the same? [Re: Growtech]
    #26473903 - 02/07/20 12:46 PM (4 years, 10 days ago)

What exactly are you saying a clone is not a twin neither are spores


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OfflineThe Mycologist
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Re: Are all the spores from a clone the same? [Re: Backbone] * 2
    #26473918 - 02/07/20 01:01 PM (4 years, 10 days ago)

Cloned isolates would be twins.


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OfflineGrowtech
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Re: Are all the spores from a clone the same? [Re: Brian Jones]
    #26474071 - 02/07/20 02:44 PM (4 years, 9 days ago)

Quote:

Brian Jones said:
I believe (correct me if I'm wrong) that even the tissue used to make a clone is made up of multiple genetics. That is why you have to make many transfers, and pay attention to sectoring, to get to an isolate.




If you get a clean tissue sample, normally it will be a single isolate.  "Normally" because mushrooms also do weird shit sometimes like a mushroom growing inside a mushroom.  But a clean tissue sample from a normal mushroom shouldn't have more than one strain going on.


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OfflineBackbone
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Re: Are all the spores from a clone the same? [Re: Growtech]
    #26474112 - 02/07/20 03:08 PM (4 years, 9 days ago)

You may find this mean but i don't understand how you analogy applies. What are the identical twins in this case, because the two mushrooms in question are exact duplicates of eachother, this stems from my lack of knowledge surely because the spores cant be mere physical attributes if they were the spores would be the same, and i mean compared between the clone and the sample not the spores themselves. So obviously some reproductive process is happening during the growth of the mushroom to produce the spores. I like to try and understand myself before i just look up all the information so forgive me. Someone on this post (sorry typing a long ass post and don't want to restart) said that a clone isolate is (in your case) the identical twin so again this is just my lack of knowledge but id assume that means that different parts of mycelium even from a clone contain different genetics but are very similar? This would make sense because the other guy here suggested that a sample contains multiple genetics. Yeah so i didn't mean all the spores are the same on an INDIVIDUAL mushroom. I just meant compared to its source i.e. the clone sample, if that's what you meant. Like someone would have made that distinction, surely, that if i cloned a mushroom that eventually senescence would kick in if i was using that mushroom to make spore syringes because in my case ( i grow mushshrooms) i'm wondering what i have to do to be self sufficient do i make syringes from a non clone culture or can i just clone and do it that way?


Edited by Backbone (02/07/20 03:23 PM)


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OfflineA.k.aM
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Re: Are all the spores from a clone the same? [Re: Backbone]
    #26474141 - 02/07/20 03:24 PM (4 years, 9 days ago)

Mushrooms are made up of multiple strains, so if you clone one you’re growing all the strains found in your donor fruit, until you do a transfer which would narrow it down.

I think he meant a cloned monoculture would be like identical twins in that a mono has one strain so cloning it would just be continuing the same strain.

I’m pretty sure that’s how it all goes down.

Spores idk, I wondered the same recently when I grew my first clones.

I think they must be more diverse because I was told to take prints from a clone grow rather than clone a clone.


Edited by A.k.a (02/07/20 03:25 PM)


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InvisibleFooMan
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Re: Are all the spores from a clone the same? [Re: Backbone] * 1
    #26474155 - 02/07/20 03:31 PM (4 years, 9 days ago)

Quote:

Backbone said:
spores arent reproduction, two spores are needed to germinate




Incorrect. A single spore will germinate fine.


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OfflineBackbone
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Re: Are all the spores from a clone the same? [Re: FooMan]
    #26474169 - 02/07/20 03:44 PM (4 years, 9 days ago)

Are you sure cus i read that spores use some hooking mechanism i could just look it up i guess this clone shit is way more obscure i feel


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InvisibleFooMan
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Re: Are all the spores from a clone the same? [Re: Backbone]
    #26474178 - 02/07/20 03:49 PM (4 years, 9 days ago)

:lol: I'm sure. The reason we use the term "multispore" is because more than one spore is used. A culture can be created from a monospore (single) spore. Workman used monospore cultures in the creation of the APE strain. Typically done by diluting spore solutions, streaking them thin onto agar then using a microscope to isolate the culture.


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OfflineA.k.aM
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Re: Are all the spores from a clone the same? [Re: FooMan]
    #26474229 - 02/07/20 04:31 PM (4 years, 9 days ago)

One spore will germ but can’t fruit right? It needs to link with another compatible one?


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OfflineBackbone
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Re: Are all the spores from a clone the same? [Re: A.k.a]
    #26474236 - 02/07/20 04:37 PM (4 years, 9 days ago)

trusted cultivator


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OfflineBlueTryptoYoshi
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Re: Are all the spores from a clone the same? [Re: Backbone]
    #26474284 - 02/07/20 05:13 PM (4 years, 9 days ago)

:popcorn:


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OfflineSockadin
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Re: Are all the spores from a clone the same? [Re: Backbone]
    #26474526 - 02/07/20 07:08 PM (4 years, 9 days ago)

Quote:

Backbone said:
Are you sure cus i read that spores use some hooking mechanism i could just look it up i guess this clone shit is way more obscure i feel





This guy just questioned Fooman......... :eww:


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Invisiblegizmo1
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Re: Are all the spores from a clone the same? [Re: Sockadin]
    #26474546 - 02/07/20 07:23 PM (4 years, 9 days ago)

Trust no one. :rofl:


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InvisibleFooMan
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Re: Are all the spores from a clone the same? [Re: A.k.a] * 1
    #26474566 - 02/07/20 07:35 PM (4 years, 9 days ago)

Quote:

A.k.a said:
One spore will germ but can’t fruit right? It needs to link with another compatible one?




No. Think of a spore more like a plant seed. The seed sprouts and you get a plant. When a spore sprouts it grows into mycelium that colonizes the substrate it germinated in. If its a single spore you get a monoculture that'll have the genetics of that single spore. But unlike plant seeds that'll grow a field of separate plants when you germinate them, the mycelium from several spores will all link up into a network of mycelium and you'll get mixed genetics of all the spores in the mix when it fruits. While mycelium from the spores can "mate" in the sense that their genes are combined they dont need to mate like a human egg & sperm do in order to reproduce.

This is a super simplified explanation but thats the jist of it


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OfflineDoyledozo
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Re: Are all the spores from a clone the same? [Re: FooMan]
    #26474573 - 02/07/20 07:40 PM (4 years, 9 days ago)

Ive been curious about all this myself. Is there any narrowing of genetics at all when you grow a cloned culture out to spores again?


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