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 Cybin_man
Circle the Wagons
Registered: 05/02/17
Posts: 799
Loc: In the bathroom
Last seen: 11 months, 13 days
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 Must be genetics 
#26474047 - 02/07/20 04:30 PM (1 year, 26 days ago) |
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It’s happening again. Clone of koh samui, 5th transfer on agar, and they are all starting to look like mutants again, this is like the third or fourth tub that did this to me. Different clones, one from spores, and they all look like shit. I love growing koh samui but I need to get some better isolated genetics.
I did a pastywhyte ez dialed tub this time, which I’m noticing a lot more growth but I can tell it don’t look right. fml
 
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mushrooms + my morning jacket = awesome https://youtu.be/xkY4isMi2Zc
  
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 Smartattack
C'mon man
Registered: 12/21/18
Posts: 1,943
Loc: A thought
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Maybe you transfered out your clone too far and lost what you had? If I get a clean plate from initial clone I like to put that straight to a master. I haven't tried taking clone plates out that many xfers before.
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Cybin_man
Circle the Wagons
Registered: 05/02/17
Posts: 799
Loc: In the bathroom
Last seen: 11 months, 13 days
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That was the farthest I went before, and I did it because my other ones were like 2 transfers and did the same thing. I did 2 separate clones and this keeps happening. I usually only transfer once or twice because they look fine after that.
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mushrooms + my morning jacket = awesome https://youtu.be/xkY4isMi2Zc
Edited by Cybin_man (02/07/20 05:27 PM)
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Smartattack
C'mon man
Registered: 12/21/18
Posts: 1,943
Loc: A thought
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Start over.
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Cybin_man
Circle the Wagons
Registered: 05/02/17
Posts: 799
Loc: In the bathroom
Last seen: 11 months, 13 days
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Lol, that was started over. From a spore print from the last batch I did. Guess I’ll start over again when my rustywhyte project is done. I have a tub of those from a spore print going along with this KS. If not I know where to get isolated genetics of some KS. If these RW turn out like these, I’m gonna flip tf out.  plus I got some melmak on agar right now also, so the koh samui might be put on the back burner.
I’ll post updated pics when these get bigger just to show y’all how they turned out, just for shi
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mushrooms + my morning jacket = awesome https://youtu.be/xkY4isMi2Zc
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TurtleEnvy
Stranger
Registered: 12/06/19
Posts: 3
Last seen: 3 months, 14 days
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Looks like bacterial blotch. I can see a couple darker streaks on stems in the Northeast quadrant of the second pic. Increase FAE and watch out for water sitting on fruits for over 4 hours.
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Cybin_man
Circle the Wagons
Registered: 05/02/17
Posts: 799
Loc: In the bathroom
Last seen: 11 months, 13 days
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Thanks, I’m not worried about that though. The clusters is what I’m talking about. Last couple tubs I had were extremely mutated, like upside down fruits, just caps growing on substrate with no stem, extremely short deformed fruits with two caps, normal looking fruit with a stem growing out the cap, shit like that. I can already tell there are just caps growing with gills on the top side.
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mushrooms + my morning jacket = awesome https://youtu.be/xkY4isMi2Zc
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TurtleEnvy
Stranger
Registered: 12/06/19
Posts: 3
Last seen: 3 months, 14 days
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Yeah. I'm pretty sure the mutations are due to the contam, not the genetics.
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jbgtaa
extraterrestrial
Registered: 06/09/19
Posts: 1,785
Last seen: 4 months, 7 days
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You’re either not cloning right or all of your cultures are extremely bacterial. Or are being introduced to bacteria during spawning or fruiting. Those look like mushrooms that tried to save themselves and reproduce but couldn’t make it.
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If the thunder don't get ya, the lightning will.
In another time's forgotten space, your eyes looked through your mother's face.
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Cybin_man
Circle the Wagons
Registered: 05/02/17
Posts: 799
Loc: In the bathroom
Last seen: 11 months, 13 days
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Re: Must be genetics [Re: jbgtaa]
#26475176 - 02/08/20 11:14 AM (1 year, 25 days ago) |
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Where the hell is the bacteria coming from? I still have transfer plates 3 and 4 that look fine, I use bucket tek coir, the grain was solid. I don’t get where the bacteria is from.. How can you take a clone wrong? Take a piece of flesh from inside the mushroom, put on agar, wait for growth, take transfer.
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mushrooms + my morning jacket = awesome https://youtu.be/xkY4isMi2Zc
Edited by Cybin_man (02/08/20 11:17 AM)
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A.k.a
Stranger
Registered: 10/27/19
Posts: 10,858
Loc: Gaming the system
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You must have sneaky bacteria, hiding in the grain or something and doesn’t make its move til the wet coir is there.
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 LAGM2020      
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Cybin_man
Circle the Wagons
Registered: 05/02/17
Posts: 799
Loc: In the bathroom
Last seen: 11 months, 13 days
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Re: Must be genetics [Re: A.k.a]
#26475317 - 02/08/20 01:14 PM (1 year, 25 days ago) |
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That’s what I’m leaning toward. When I do a shake at like 30% it only takes a few days to fully colonize after that, and it looks super clean so I don’t know wtf. Just bums me out.
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mushrooms + my morning jacket = awesome https://youtu.be/xkY4isMi2Zc
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jbgtaa
extraterrestrial
Registered: 06/09/19
Posts: 1,785
Last seen: 4 months, 7 days
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Wet anything will bring out whatever contams are present. And bacteria can come from literally anywhere. Very well could not be your fault.
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If the thunder don't get ya, the lightning will.
In another time's forgotten space, your eyes looked through your mother's face.
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Forever giving away prints. PM at anytime for a free print.
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Cybin_man
Circle the Wagons
Registered: 05/02/17
Posts: 799
Loc: In the bathroom
Last seen: 11 months, 13 days
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Re: Must be genetics [Re: jbgtaa] 1
#26484243 - 02/13/20 09:11 PM (1 year, 20 days ago) |
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I said I would update this with a picture, so here it is, this was after I cut some down. Everything is cleared out, I’ll see if the second flush is any better, I doubt it.
My rustywhyte tub is looking promising though. I did everything the same with that except it was only 2nd transfer from a spore print.
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mushrooms + my morning jacket = awesome https://youtu.be/xkY4isMi2Zc
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Cybin_man
Circle the Wagons
Registered: 05/02/17
Posts: 799
Loc: In the bathroom
Last seen: 11 months, 13 days
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This is what I meant by it must be genetics. I did everything the exact same for both of these tubs except the koh samui was a clone from a multi spore run, I’ve had these same KS genetics for years making new spore prints and such, and the last like 3 or 4 tubs turned out mutated and weird.
The Rustywhyte came from a print that I bought and it did great.
Both worked on agar, same grain spawn, same substrate, same tub.
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mushrooms + my morning jacket = awesome https://youtu.be/xkY4isMi2Zc
Edited by Cybin_man (02/26/20 08:06 PM)
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