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OfflineBackbone
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Registered: 01/18/20
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how many times can you clone from a single petri dish also how many times can you transfer in agar?
    #26472070 - 02/06/20 01:25 PM (3 years, 11 months ago)

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OfflineElrik
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Registered: 08/29/19
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Re: how many times can you clone from a single petri dish also how many times can you transfer in agar? [Re: Backbone]
    #26472083 - 02/06/20 01:33 PM (3 years, 11 months ago)

How long is a piece of string?

You can keep taking transfers from a plate until it contams. At the least I try to keep Tx-1 plates around until the new plates are clearly growing uncontaminated.

In terms of numbers of sequential transfers, the mycelium eventually gets old and decrepit. This is why master slants are made, and then used to make petri masters, which are used to make petris. So you have a very long time before your stuck with a T20.


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