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Yumyumyumyum
Thinker


Registered: 12/21/19
Posts: 412
Loc: PacNW
Last seen: 5 months, 17 days
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Anyone keep track of their prints and agar genetics?
#26470702 - 02/05/20 04:59 PM (4 years, 11 days ago) |
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I started with a prime that turned into 4 cakes producing 14 unique samples and plates with different combinations based on what grew. Is this effective? Or am I dealing with the same genetic card from the first sample? Are the mushrooms altered by condition and print that strength to the spores they produce? I also have moisture in some of my agar and the temp is only in the low 70's. All my pads are being used for fruiting and inoc. I do not want to combine my plates into the same storage as my jars in fear of contamination.
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InfiniteDreams


Registered: 10/25/19
Posts: 1,224
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Re: Anyone keep track of their prints and agar genetics? [Re: Yumyumyumyum]
#26470722 - 02/05/20 05:10 PM (4 years, 11 days ago) |
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Yes?
Do you have 14 tissue samples or 14 new prints?
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Yumyumyumyum
Thinker


Registered: 12/21/19
Posts: 412
Loc: PacNW
Last seen: 5 months, 17 days
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Re: Anyone keep track of their prints and agar genetics? [Re: InfiniteDreams]
#26470774 - 02/05/20 05:32 PM (4 years, 11 days ago) |
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4 prints, 4 inner stem samples 2 cap samples and a few liquid cultures of the different combinations.
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spiritlands



Registered: 06/21/10
Posts: 1,616
Loc:
Last seen: 2 months, 14 days
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Re: Anyone keep track of their prints and agar genetics? [Re: Yumyumyumyum]
#26470787 - 02/05/20 05:38 PM (4 years, 11 days ago) |
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I leave my agar to colonize in the low 70s, it's fine. When you make your agar let it cool down before you pour. Depends if you're doing pour or no-pour plates. My no-pour pitris I leave open on the counter until it's cool and solid before putting the lids on and pc. This helps a lot with condensation.
 
From multispore your gonna see lots of variability. If you see one you like clone it and take prints off the original and the clones that come after. I select for more than looks. I select for clusters, spore production, most vigorous fruits in the conditions your fruiting under, and phenotype. I go in that order and I try to find a couple that match all the criteria. Then I only pick the best cultures from those clones.
You can keep track of as much as you want to but unless there's a reason to its not necessary. If you get in the habit of only keeping the best of everything all your stuff will be quality and your mush cult will be easier.
As for cross contaminating your jars and agar, idk.. Your jars should be only inoculated with tissue that is clean on agar first even if you decide to use lc/li or whatever. Your agar might contam but if it's jumping from agar to jars just by being close to each other then it's a sterile technique issue, not bc they were in the cupboard together.
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Yumyumyumyum
Thinker


Registered: 12/21/19
Posts: 412
Loc: PacNW
Last seen: 5 months, 17 days
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Re: Anyone keep track of their prints and agar genetics? [Re: spiritlands]
#26470804 - 02/05/20 05:47 PM (4 years, 11 days ago) |
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Thank you,
None of my agar has developed yet. I injected my jars with print spores and various parts soaked in DW. I know that is a mistake now because of the contamination. My dishes started on the 31st and starting to look interesting some looks like blister, others look like they are degrading with white fuzz on the pieces. I assume this the start of what it will eventually turn into. I am hoping by the time they develop the jars will take or not take. I have all the stuff for producing jars. I have been taking the best samples and converting them. 4X metrics stem,cap,potperg,size. I am keeping a spreadsheet on their origin, process and base sample.
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