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Offlinenajohns
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Registered: 12/28/19
Posts: 14
Last seen: 4 months, 21 days
First Time Agar Adventure
    #26470245 - 02/05/20 12:49 PM (3 years, 11 months ago)

So, I'm finally attempting the switch from BRF to bulk beginning with MEA.  I started here with testing a B+ spore syringe. One drop per plate onto five plates. Ten days later, I've got five plates looking about like this. All plates germinated exactly where the drop was placed with slow wispy growth, no contamination anywhere else. So, Mold or Myc?





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Offlinefootpath
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Registered: 07/16/19
Posts: 1,367
Last seen: 1 year, 2 months
Re: First Time Agar Adventure [Re: najohns]
    #26470272 - 02/05/20 01:02 PM (3 years, 11 months ago)

Both a contaminant and cube mycelium.
May very well be bacteria, not necessarily mold. Let it grow a bit more to try to get something more distinguishable.

edit: What was your agar recipe?


Edited by footpath (02/05/20 01:03 PM)


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Offlinenajohns
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Registered: 12/28/19
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Re: First Time Agar Adventure [Re: footpath]
    #26470284 - 02/05/20 01:08 PM (3 years, 11 months ago)

10 g  Agar Powder
10 g Barley Malt Extract
1 g Nutritional Yeast
500ml Water


Edited by najohns (02/05/20 01:09 PM)


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Offlinefootpath
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Re: First Time Agar Adventure [Re: najohns]
    #26470327 - 02/05/20 01:29 PM (3 years, 11 months ago)

Pretty standard.
It doesn't spread as quickly with higher nutrient content, but I wouldn't say that's very high. So, I would still suspect contaminant presence.
Either way, you'll know better where to transfer from in a couple days. Looks on par for a syringe drop.

I tend to do my recipes with 7g light malt extract/10g agar/500mL H2O - drives the culture out a bit faster.


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Offlinenajohns
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Registered: 12/28/19
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Last seen: 4 months, 21 days
Re: First Time Agar Adventure [Re: najohns]
    #26478918 - 02/10/20 04:55 PM (3 years, 11 months ago)

Here's an update:

Pictured here is an updated photo of the plate from the original post. I took a wedge out of it and made a transfer to another plate with a new agar recipe per footpath's recommendation: 7g LMA/10g Agar/500ml H2O. Transfer is pictured also and is showing much better rhizo growth:

Updated original plate:


1st Transfer top view:


1st Transfer bottom view:


Does this inform any of you on whether or not there was contamination on the first plate? There's quite obvious Rhizo growth on the transfer. Should I do a 2nd transfer once the plate fills out a bit more, and if so, what section looks best to cut from?


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OfflineSynKyd
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Registered: 09/27/13
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Re: First Time Agar Adventure [Re: najohns]
    #26479157 - 02/10/20 07:20 PM (3 years, 11 months ago)

Give it time to grow out and take another pic....:cheers:


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Offlinenajohns
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Registered: 12/28/19
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Last seen: 4 months, 21 days
Re: First Time Agar Adventure [Re: SynKyd]
    #26481790 - 02/12/20 11:11 AM (3 years, 11 months ago)

Here's an updated picture of the transfer. With my untrained eye I'm having a hard time determining if there's a white mold-like contaminant or if this is looking normal.



What do you think?


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OfflineBackbone
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Registered: 01/18/20
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Re: First Time Agar Adventure [Re: najohns]
    #26481807 - 02/12/20 11:20 AM (3 years, 11 months ago)

it looks fine to me


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InvisibleMr Solo Dolo


Registered: 02/24/19
Posts: 243
Loc: U.S
Re: First Time Agar Adventure [Re: Backbone]
    #26481836 - 02/12/20 11:38 AM (3 years, 11 months ago)

Looks like myc to me
:howyoudoing:


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