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Offlinemetalcore_dad
A Cold Shower
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Registered: 02/05/20
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What the heck is up with my jars?
    #26470084 - 02/05/20 11:00 AM (3 years, 11 months ago)

Hey folks,

First off just wanted to say wow, been so amazed by the amount of information and knowledge everyone has on mycology/shroom tek. I'm a complete beginner to this stuff, minus the obsessive amount of reading and forum browsing I've been doing. Aside from that, I have little practical experience.

This is my second grow that has not gotten past the BRF jar stage due to what I suspect is trich contam in every jar.

First go around, my sterile technique was not optimal, (used a glove bag, it popped, you do the rest), so it was reasonable to assume every jar could, and did get the trich. It took 2 weeks to show trich, with no mycelial growth whatsoever.


Second go around, I inoculated with 2x the amount of spores, to speed up growth, only to find that TWICE the amount of trich ended up growing and still no sign of mycelium. This time I used optimal sterile technique: I wore a lab gown, made a SAB, double gloved, everything I could do to ensure sterility. Again, EVERY jar contaminated with what I believe to be trich mold.


What is going on? Contaminated syringe? Sterilization failure? Why am I seeing trich before mycelium? I'm going to run 1 blank, and 3 jars with 3 different syringes to see if my entire batch of syringes is contaminated. If blank gets contam, that tells me it's my sterilization, if none, then syringes likely culprit.


Can you corroborate that this is in fact, trich mold? Pictures below:



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OfflineBilever
Cthulhu
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Registered: 08/21/17
Posts: 164
Loc: Las Vegas, Nevada, United Stat... Flag
Last seen: 7 months, 8 days
Re: What the heck is up with my jars? [Re: metalcore_dad]
    #26470240 - 02/05/20 12:43 PM (3 years, 11 months ago)

I hate brf it seems to contaminate really easy for me. Id suggest getting rid of that cloth tape in favor of self healing injectable ports. Next time try using wild bird seed in my experience cubensis mycelium seems to spread faster on it. Also make sure your heating the jars enough to pasteurize the contents before inoculating. If you really wanna beat out contaminations you should try growing the mycelium in petri dishes it will save you a lot of headaches. And make sure you are getting your spores from a good vendor some vendors spores have contaminants in it but generally the shroomery sponsors have good spores


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