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Packerbacker12
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Lions mane or contaminate?
#26469989 - 02/05/20 10:01 AM (3 years, 11 months ago) |
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Hello everyone,
So my lion's mane has given me quite the time. I originally started with spawn from Aloha. I took a few kernels of grain and transferred them to a plate. Heres what I got:

from there I transferred again a few more times and ended up with this:

I thought I was looking golden so I transferred it over to some liquid culture. I then inoculated some grains and made about 8 supplemented sawdust bags. All 8 bags colonized quickly but then became a orange ish tint. I have grown lions mane before and know how quickly you start to get early fruits. Not in this case. My blocks stayed a mild orange for another month and never fruited.
So I decided to test my original LC jar and heres what I got:
heres what my grain jars look like:

A dead give away that something is wrong is the fact that I have now noticed at all phases this mycelium smells sweet. Like over ripe apricots. All of my current "lions mane" grain jars smell this way and even the old plates that I pulled from the fridge.
Now that I look at the plates again, I am very worried I transferred a contaminate of some sort and have been growing it all along.
If any body can help that would be great!
Thanks!
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Packerbacker12
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Registered: 08/26/18
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Anyone who can solve this?
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Maylink
One with Gratitude



Registered: 08/29/19
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Try posting in the Gourmet and Medicinal Cultivation forum. I’m sure you’ll get more responses there.
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Hillionaire
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Re: Lions mane or contaminate? [Re: Maylink]
#26476213 - 02/08/20 10:19 PM (3 years, 11 months ago) |
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Youve definitely got some other fungus or biology growing. The lions mane is a prolific agar specimen, white.
Maybe cinnamon mold?
http://en.psilosophy.info/what_are_common_contaminants_of_the_mushroom_culture.html
I had some pop up on grain bags. Cool colors, no bueno growth. I had lipstick mold too... just in a garage.
A few of the lions mane pushed through and crushed it. But not for long... the media was too wet and sooner or later... kaput.
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Psychgro
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Registered: 12/21/19
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Re: Lions mane or contaminate? [Re: Hillionaire]
#26482330 - 02/12/20 05:00 PM (3 years, 11 months ago) |
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Well, after following that link I googled one of the names and found this link with at least 3 look alike mushroom mycelium 😔
https://iijls.com/currentissue/Html-1601.htm
-------------------- Looking to learn all about it
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Packerbacker12
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Re: Lions mane or contaminate? [Re: Psychgro]
#26483377 - 02/13/20 09:47 AM (3 years, 11 months ago) |
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Thank you guys for showing interest in this! I waited a bit longer before I cleared them out but my worst fear was true. All of what I thought was my LM culture turned out to be something totally different with very similar characteristics. My culture would not fruit on anything. Plates, grain jars or sawdust bags. I know from growing Hericium in the past that this just isn't right. At almost all stages, LM wants to grow! Plus all of my plates, LC, Jars and bags all have that sweet overripe apricot smell. It cant be from the grains because all of my other cultures are rocking out on the same grain!
oh well! Time to start over with hericium
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Hillionaire
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I’ve noticed pretty consistently that anything in agar will avoid each other and grow around each other’s mycelium. In liquid culture I’d imagine you’d have some strict anaerobes, some that can go both ways, and some strict aerobes that won’t grow in still liquid culture. So you will see floaters.
From my limited experience but more book worthy knowledge, the stuff floating is either dead mycelium or something else because without even looking in a book I can tell they are not obligate aerobes, but can also be anaerobes. They float at the bottom in mass for days and still grow with aeration, even proliferate like yeast when oxygen is increased in solution. I won’t even bother with liquid culture anymore without a stir plate going non-stop. Masses grow in days not weeks. So much better.
Lastly, in the growing substrate again the microbes and biota seem to know each other and avoids one another. You can see green patches next to fruiting pins next to bald spots which I imagine could be bacteria but I’ve yet to take the time to plate and stain to check.
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Hillionaire
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Re: Lions mane or contaminate? [Re: Hillionaire]
#26511993 - 03/01/20 09:06 PM (3 years, 10 months ago) |
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I got a liquid culture from mycelium emporium, that was off the hook. Definitely took to the substrate and grain real quick. I went directly into coco/vermiculite/ewc just to see if I could.
Worth a shot and it’s like 15$. Just microwave some mea or yeast hulls with just about any sugar solution @ 2%. Boil with lid slightly loosened and wham... easy liquid culture. Tighten as soon as you open the door to the microwave just snug, and the cooling process with vacuum it up so damned tight you’ll need an oil filter strap wrench to clean it out.
I actually had to brace myself to inject a cc into the jar bc it nearly ripped my thumb off sucking out the syringe liquid.
Pretty good stuff... just use a small stir bar in the 750ml or half gallon jars and it’s hella easy.
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