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Fireneeb
Stranger
Registered: 10/28/18
Posts: 93
Last seen: 3 years, 8 months
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Starting to lose my mind. Trich, or what is this
#26469532 - 02/05/20 12:46 AM (3 years, 11 months ago) |
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So I’ve been at it about a year now. Had contams the first 6 months, but after getting my sterile technique down and other issues, the last few months have been filled with lots of success. Until my last 2 batches. Usually I clone, 1-2 transfers, A2G, G2G. With another agar transfer to a new plate for the next batch. When doing G2G I Pressure cook 14 q jars and do them all the same night in the SAB. So last couple batches I’m running a 50% contam rate compared to my usual 2-6%. It always starts off a couple grains looks cobwebby and then it turns green (trich I’m assuming). I bought a new SAB incase mine was cracked and have my sterile tek turned up to 11. But I still ran 50% on my last round of jars. Every time it contams however it is always the same 7 jars that would have come from the same master jar. But all my masters jar are colonizing cleanly (or so it looks). I’ve come to the conclusion that my first A2A transfer off the clone carried a hidden contam and I have continued transferring it. It’s iust frustrating I never see the contams until G2G and wasting my time pressure cooking the jars lol. So I’ve done a new clone and going to do 4 transfers before growing thenolayes out and using them. I’ve included photos below, basically just want confirmation on what I’m thinking.
TLDR; random batches of G2G jars contaminated even tho the master jars appear and smell clean. Assuming hidden trich carried from my agar plates. I also had 2 A2G jars contam this week but I’m assuming it was from the same conetammed plate since I split 1 plate into 2 jars. Thanks!


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Maylink
One with Gratitude



Registered: 08/29/19
Posts: 320
Loc: everywhere and nowhere
Last seen: 6 months, 27 days
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Re: Starting to lose my mind. Trich, or what is this [Re: Fireneeb]
#26471263 - 02/05/20 11:21 PM (3 years, 11 months ago) |
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Jars look wet, what’s your grain prep?
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Fireneeb
Stranger
Registered: 10/28/18
Posts: 93
Last seen: 3 years, 8 months
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Re: Starting to lose my mind. Trich, or what is this [Re: Maylink]
#26473062 - 02/06/20 11:39 PM (3 years, 11 months ago) |
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Sorry I took these photos outside before dumping them, I think they just look wet from the condensation from being in the cold. When inside they don’t look as wet at all haha. I do cold water in oats, bring to a boil, let sit for 20 with lid on then strain for about 12 hours. My strainers aren’t great, I’m going to build one soon that has more surface area however my drains are never that wet usually. Did my second transfer today from the new clone, doing the 3rd in a couple more days. Hoping it was hidden contams in my master jars. Ps sorry the photos are so Big, idk how to fix that haha
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