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SporeJunkie
Pöåšt Šhïttēr



Registered: 11/30/18
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Give me the bad news
#26469513 - 02/05/20 12:31 AM (3 years, 11 months ago) |
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I posted this in mush cult like a dumb ass. I guess i was stoned and forgot about the contam forum. Anyway, i didnt get much of an answer there. Pretty to the point. Pretty sure this is bad but i like second opinions. Thanks in advance.
-------------------- Royale with cheese
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SporeJunkie
Pöåšt Šhïttēr



Registered: 11/30/18
Posts: 2,106
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So 20 people have looked and no one has given any input at all so far. Wow thanks dudes. So helpful.
-------------------- Royale with cheese
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MrBovineJoni
Glip-Glop

Registered: 11/28/19
Posts: 264
Last seen: 13 days, 14 hours
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You don't ask a proper question and are getting butthurt that no one has bothered to answer?
What is in the pictures? (strain, how long it has been growing). Was this multispore inoculation, was is agar transfer, clone etc? What are you growing this for - to transfer (too late for that) or inoculate grains (I would also say too late for that as well if you want to be safe).
It does not look great but not the worst thing as well - if this was spores to agar (very overgrown), it looks how I would expect - but still would need at least a transfer or two before putting it on grains. If this was from an agar transfer it looks pretty bad.
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SporeJunkie
Pöåšt Šhïttēr



Registered: 11/30/18
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Well its cube i figured thatd be a given unless specified otherwise. However, if those reasons are really enough to cause people not to reply then I apologize. The question is literally how does it look or is it contaminated.... and its print to agar.
-------------------- Royale with cheese
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MrBovineJoni
Glip-Glop

Registered: 11/28/19
Posts: 264
Last seen: 13 days, 14 hours
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Quote:
SporeJunkie said: Well its cube i figured thatd be a given unless specified otherwise. However, if those reasons are really enough to cause people not to reply then I apologize. The question is literally how does it look or is it contaminated.... and its print to agar.
Technically you never asked a question in your original post. Just a bunch of statements irrelevant to the contents of your pics 
Anyhow, it looks as I would have expected for MS (multi spore) to agar initial inoculation. But as I said before you should have transferred much earlier - the rule of thumb is to do it before growth reaches edges (e.g. 60-80% of the plate is covered). Then you would not have to ask if it is contaminated - with more organised transfer (single point of origin in the center) it would be much easier to spot something that is looking bad.
Some of this mycelium grow could be hiding some bacteria but it is way too overgrown and chaotic to be sure now just by looking. However, I have seen much worse and it doesn't look very bad either, but I would never use this to inoculate grains directly without cleaning it up by transfers.
Does this answer your "question" ?
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SporeJunkie
Pöåšt Šhïttēr



Registered: 11/30/18
Posts: 2,106
Loc: TheOnlyTenISee
Last seen: 2 months, 12 days
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Kinda but its looked like that from the very beginning. The only thing thats changed is the surface area. The spores are from a Puerto Rican print that i swabbed some of. I didnt transfer or anything mainly because i just wanted to try agar once to see if i could manage it. I cant grow at the moment so i never had any intention of taking it any further for the time being. I do appreciate your advice man but come on you cant tell me that my first post wasnt pretty obvious.
-------------------- Royale with cheese
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ape2
Stranger

Registered: 01/29/20
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If you are going to grains or agar, don't wait for the plate to fully colonize. It makes it harder to spot contamination and to take a healthy wedge. You should still be able to see an edge when you take your agar wedge.
It should look something like this picture when you take a wedge, even if you streak your plate (which you should if using spores from print or syringe).
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