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Zillard
Stranger

Registered: 01/28/20 
Posts: 16
Loc: South Africa
Last seen: 3 years, 9 months
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Novice eyes need some training... please
#26469454 - 02/04/20 11:21 PM (3 years, 11 months ago) |
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Morning all
A few days ago i put some scrapings i took from a sample mushroom onto some freshly prepared agar.The sample Golden Teacher was kind of small, and extremely dry. The inside fibers of the stem was flakey to the touch, and easily fell out onto the paper i placed for collection.
I dropped a few of the flecks onto the agar, and sealed the petri dish with some microporous tape, and stored it upside down so no moisture could drop onto the agar.
Yesterday morning i noticed that one of the flecks had a little bit of white fluff forming around it, but nothing else visible on the plate (apart from old dry fibers).
Yesterday afternoon i took this picture, as there were some extra growth on the plate:

The black piece at the very bottom was the fluff i noticed earlier in the day. The fluff just above it was new, and the large brown/yellowish section top right corner was also new. When i say brown/yellowish i mean that the center of it had some yellowish color, very faint though, and the outside was white.Decided to leave it and check again later on.
This morning i found that the initial piece is slightly larger:

The small fluff that was above it is now green, so i'm guessing mold. The larger section that was yellow in the center now has a white center and yellowish edges.
As this is my very first attempt at doing any mycology, i need some assistance with identification.
I think that the initial fluff around the flake of fiber is healthy mycelium. The green is mold, and the yellow larger growth is some other form of contamination.
Would the more experienced growers please shed some light for me, so i can see if my assumptions are correct?
I have planned to transfer growth from this dish to another 2 at least to improve the chances of getting clean mycelium, however i don't want to transfer contaminates thinking they are healthy mycelium.
Awaiting your feedback, thank you all.
PS - I went through similar posts to mine, however am unable to see the pictures clear enough to try and identify mine. I also hope my pictures are good enough to be able to do so. It is hard for me to focus my phone on the bits that need it. These pics were the best of the lot.
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Geinstein
Shroomery addict



Registered: 01/25/18
Posts: 1,784
Last seen: 2 years, 1 month
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Re: Novice eyes need some training... please [Re: Zillard]
#26469495 - 02/05/20 12:10 AM (3 years, 11 months ago) |
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Hey my South African friend didn't read all that just looking at photos.
Its contam
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Nothing breads nothing
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Zillard
Stranger

Registered: 01/28/20 
Posts: 16
Loc: South Africa
Last seen: 3 years, 9 months
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Re: Novice eyes need some training... please [Re: Geinstein]
#26469511 - 02/05/20 12:29 AM (3 years, 11 months ago) |
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Ok. All of it?
Was thinking the bit that started growing first around some fiber would be usable if transferred to fresh agar.
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Kmacmo
The aborted pin



Registered: 08/14/19
Posts: 1,675
Loc: Central hemisphere
Last seen: 7 hours, 9 minutes
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Re: Novice eyes need some training... please [Re: Geinstein]
#26469516 - 02/05/20 12:33 AM (3 years, 11 months ago) |
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Yeah unfortunately it's no good I'd say. Looks like a few kinds of different molds there I wouldn't bother trying to get anything off it just try again and don't give up!
You'd be better getting a print and working with that, clones contaminate quite easily not going to give you much confidence but spores will do you well
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Zillard
Stranger

Registered: 01/28/20 
Posts: 16
Loc: South Africa
Last seen: 3 years, 9 months
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Re: Novice eyes need some training... please [Re: Kmacmo]
#26469520 - 02/05/20 12:35 AM (3 years, 11 months ago) |
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Aah, ok.
Thanks guys. Was really hoping it would work right off the bat, but alas, no joy.
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