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funguy



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Purple mold?
#26469429 - 02/04/20 10:59 PM (3 years, 11 months ago) |
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Hi, I inoculated my brf jars about a week ago and they're all showing this strange purple stuff at the inoc points and even at the bottom of one of the jars. I looked it up and got really paranoid about it being toxic mold, but I wanted some advice on that before I toss em. It doesn't look very similar to the posts that I've seen. Also, how can I avoid this in the future? Am I gonna die now cause I sniffed the air in the box? Lol. TIA
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Edited by toads (02/05/20 09:31 AM)
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Maylink
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Re: Purple mold? [Re: toads]
#26471282 - 02/05/20 11:40 PM (3 years, 11 months ago) |
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Funky. Toss them yesterday.
Follow the TEK you’re using to a T and up your sterile procedure. And most of all, learn and try again!
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funguy



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Re: Purple mold? [Re: Maylink]
#26471687 - 02/06/20 08:45 AM (3 years, 11 months ago) |
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Will do, thanks! I'm considering testing the syringe for contamination with agar. Got started recently but I'm really enjoying this hobby and this board
-------------------- uh oh stinky
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Maylink
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Re: Purple mold? [Re: toads]
#26471813 - 02/06/20 10:35 AM (3 years, 11 months ago) |
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Agar is the way to go. Syringes are often dirty. I concur that this hobby and the Shroomery are awesome too.
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hazyhorse
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Re: Purple mold? [Re: Maylink]
#26471933 - 02/06/20 12:11 PM (3 years, 11 months ago) |
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how did you sterilize the jars? it could totally be from improper sterilization, a dirty syringe could also be the culprit. if you bought a syringe from a reputable vendor on here it should be clean... but there’s never any guarantee i suppose
you should def work with agar & grains though, if you’re feeling up to it. PF was nice when i didn’t have a PC but grains & agar are definitely the way to go. the learning curve isn’t really that difficult either
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funguy



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Quote:
hazyhorse said: how did you sterilize the jars? it could totally be from improper sterilization, a dirty syringe could also be the culprit. if you bought a syringe from a reputable vendor on here it should be clean... but there’s never any guarantee i suppose
you should def work with agar & grains though, if you’re feeling up to it. PF was nice when i didn’t have a PC but grains & agar are definitely the way to go. the learning curve isn’t really that difficult either
I sat them in boiling water for more than an hour (with the verm and brf already inside). I'm trying the agar thing today, fingers crossed I can salvage this syringe!
-------------------- uh oh stinky
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hazyhorse
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Re: Purple mold? [Re: toads]
#26473110 - 02/07/20 12:40 AM (3 years, 11 months ago) |
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you may have wanted to steam them a bit longer, still coulda been the syringe idk. agar is gonna be a great way to clean up anything that might be going on! what tek are you planning on using?
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funguy



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Quote:
hazyhorse said: you may have wanted to steam them a bit longer, still coulda been the syringe idk. agar is gonna be a great way to clean up anything that might be going on! what tek are you planning on using?
Yesterday I put some spore solution onto an agar plate with ampicillin just because I have access to those. 
After that, I would probably follow this tek for inoculating jars once I've done a few transfers. Considering not doing verm/brf and maybe just using grain, but then what would I do with my extra brf?
-------------------- uh oh stinky
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hazyhorse
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Re: Purple mold? [Re: toads]
#26473878 - 02/07/20 12:28 PM (3 years, 11 months ago) |
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nice! i don't know much about using antibiotics in agar but i wish you luck. tbh i would recommend grains over BRF any day. if you have a pressure cooker it's a no brainer. it does blow if you get left with a bunch of verm & BRF but oh well i guess
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funguy



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Quote:
hazyhorse said: nice! i don't know much about using antibiotics in agar but i wish you luck. tbh i would recommend grains over BRF any day. if you have a pressure cooker it's a no brainer. it does blow if you get left with a bunch of verm & BRF but oh well i guess
Thank you! I've been looking at pressure cookers but they're kinda pricey for the larger sizes. Maybe I'll try the thrift store. Do you have any pressure cookers you recommend?
-------------------- uh oh stinky
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hazyhorse
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Re: Purple mold? [Re: toads]
#26474858 - 02/08/20 12:40 AM (3 years, 11 months ago) |
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definitely check the thrift stores!! i’ve never seen a 16 or 23qt at any of the ones near me but that doesn’t mean you can’t find them. you’ll probably see a lot of prestos, they’re solid PCs. all americans are apparently killer but usually pricey new
my personal PC is a 6 or 8 quart presto (can’t remember). i got it for $10 with the weight. a new weight & gasket are like $10 each. i can fit 4 pint jars (equaling 2 quarts) but can only do 1 quart jar. if you don’t need to grow a fuck ton, you can make the 6qt PC work. usually thrift stores have jars too, so you can actually see if the PC would work for you
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Maylink
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Re: Purple mold? [Re: toads]
#26474880 - 02/08/20 01:22 AM (3 years, 11 months ago) |
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Quote:
toads said:
Quote:
hazyhorse said: nice! i don't know much about using antibiotics in agar but i wish you luck. tbh i would recommend grains over BRF any day. if you have a pressure cooker it's a no brainer. it does blow if you get left with a bunch of verm & BRF but oh well i guess
Thank you! I've been looking at pressure cookers but they're kinda pricey for the larger sizes. Maybe I'll try the thrift store. Do you have any pressure cookers you recommend?
also check OfferUp or FB Marketplace, lots of good cheap stuff there.
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toads
funguy



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Re: Purple mold? [Re: Maylink]
#26475203 - 02/08/20 09:37 AM (3 years, 11 months ago) |
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Thank you both so much for the advice, yeah I think I won't be growing much at first at least. Idk if it was because I ate too much, but yesterday some edibles gave me a killer headache and now I'm worried the mushrooms will do the same or something. Will keep you updated with agar pics!
-------------------- uh oh stinky
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hazyhorse
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Re: Purple mold? [Re: toads]
#26475527 - 02/08/20 01:38 PM (3 years, 11 months ago) |
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lmao whenever i smoke too much i end up getting super anxious & worries tripping will do the same. but then i take mushies & it’s bliss lol
if you’re not gonna be growing a lot, a small PC from a thrift store should serve you well. only being able to do 2qt of spawn at a time sounds shitty, but if you’re doing shoeboxes that’s one shoebox right there. you do a few runs of grain during the days while your agar plates are colonizing you can easily have 6+ quarts of spawn by the time you need it
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Maylink
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Re: Purple mold? [Re: toads]
#26475552 - 02/08/20 01:56 PM (3 years, 11 months ago) |
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Quote:
toads said: Thank you both so much for the advice, yeah I think I won't be growing much at first at least. Idk if it was because I ate too much, but yesterday some edibles gave me a killer headache and now I'm worried the mushrooms will do the same or something. Will keep you updated with agar pics!
Cannabis vs mushrooms different animal, so I wouldn't worry. But it is important you are in the right headspace. Also, fasting 12-25 hrs does wonders (for the shrooms).
Keep us posted with your agar work!
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hazyhorse
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Re: Purple mold? [Re: Maylink]
#26475578 - 02/08/20 02:11 PM (3 years, 11 months ago) |
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Quote:
Maylink said:
Cannabis vs mushrooms different animal, so I wouldn't worry. But it is important you are in the right headspace. Also, fasting 12-25 hrs does wonders (for the shrooms).
Keep us posted with your agar work!
head space is huge!! i also agree with the fasting. personally i don’t time it out (& i don’t think i would want to go 24 hours without food either) but i know a lot of people swear by it. the way i “fast” is just by not eating in the morning & taking my dose within 2 hours of being awake. i think you technically only need to “fast” 3 or 4 hours until all the food has left your system, but waking up & tripping is my preferred method bc the day doesn’t have a chance to add any baggage or feelings going into the trip
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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funguy



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Re: Purple mold? [Re: Maylink]
#26478811 - 02/10/20 03:46 PM (3 years, 11 months ago) |
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Quote:
Maylink said:
Quote:
toads said: Thank you both so much for the advice, yeah I think I won't be growing much at first at least. Idk if it was because I ate too much, but yesterday some edibles gave me a killer headache and now I'm worried the mushrooms will do the same or something. Will keep you updated with agar pics!
Cannabis vs mushrooms different animal, so I wouldn't worry. But it is important you are in the right headspace. Also, fasting 12-25 hrs does wonders (for the shrooms).
Keep us posted with your agar work!
Its been like 4-5 days and the agar where I put the spore solution turned a darker shade of yellow-orange than the rest of the plate but nothing else has changed. Is that normal? I've been googling it and can't find much on that.
-------------------- uh oh stinky
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hazyhorse
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Re: Purple mold? [Re: toads]
#26479092 - 02/10/20 06:43 PM (3 years, 11 months ago) |
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don’t worry about it yet. my agar changes color after mycelium starts to colonize it. it goes from green to washed out piss yellow lol. it could be a contam since there’s no pic attached, but i’d give it a few more days before you start to worry. plus, as long as some spores have germinated you’ll transfer that growth to new plates anyways. hopefully you see some myc pop up in a few days!! i noticed myc on my agar spore plates after about 6 days, but this can take longer depending on temps & spore vigor & all that
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funguy



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Quote:
hazyhorse said: don’t worry about it yet. my agar changes color after mycelium starts to colonize it. it goes from green to washed out piss yellow lol. it could be a contam since there’s no pic attached, but i’d give it a few more days before you start to worry. plus, as long as some spores have germinated you’ll transfer that growth to new plates anyways. hopefully you see some myc pop up in a few days!! i noticed myc on my agar spore plates after about 6 days, but this can take longer depending on temps & spore vigor & all that
Here's a pic
I was hesitant to upload one because I flubbed up putting a drop on there and ended up adding like a whole cc. That's the last time I try to use one hand. I was SO mad, this is my only GT syringe and now there's about 1 cc left. Though I'm starting to think I didn't get a good spore print or something, cause first my brf jars ALL got contaminated, and now I'm not seeing anything on a plate. Lots o failures.
-------------------- uh oh stinky
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Maylink
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Re: Purple mold? [Re: toads]
#26479418 - 02/10/20 11:31 PM (3 years, 11 months ago) |
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RR’s signature line:
Quote:
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Think of every failure as an opportunity to learn? And when you have success, it will be that much greater 
And probably a dirty syringe. Are you able to clean it up on agar?
Edited by Maylink (02/10/20 11:34 PM)
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funguy



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Re: Purple mold? [Re: Maylink]
#26479421 - 02/10/20 11:34 PM (3 years, 11 months ago) |
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Quote:
Maylink said: RR’s signature line:
Quote:
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Think of every failure as an opportunity to learn? And when you have success, it will be that much greater 
Will do! I feel better having advice from people here. Tysm 
and nope. still no growth on antibiotic agar. May try making my own agar without antibiotics next, if nothing happens.
-------------------- uh oh stinky
Edited by toads (02/11/20 12:20 AM)
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hazyhorse
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Re: Purple mold? [Re: toads]
#26479508 - 02/11/20 02:47 AM (3 years, 11 months ago) |
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LOL i feel that so hard, i hate using syringes. i always fuck them up. swear to god i used like 4cc on one of my first PF jars on accident lol
don’t sweat it!! my personal philosophy is it works when you need it to (of course, good technique & some experience go a long way in making that smoother ;D). you’ll get it
as far as your agar goes... dunno what the dark spot is but i assume it has something to do with the water from the syringe. give it some time. spores take awhile to germinate & get going. the antibiotics may have something to do with it but i’m not sure. you should see some mycelium eventually. if this syringe is as dirty as it seems, you’re gonna get plenty of experience w agar (;
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Edited by hazyhorse (02/11/20 02:48 AM)
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fragrantdiscipline
LostWanderer


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Quote:
I sat them in boiling water for more than an hour
Boiling will kill most things. It won't kill everything. A pressure cooker is necessary to sterilize.
Quote:
Do you have any pressure cookers you recommend
I have a 6 quart pressure cooker. It serves my needs just fine. It's a good starting point and they can be had for cheap. It's definitely enough unless you need to sterilize a ton of stuff. I don't foresee needing something like a 23 quart pressure cooker unless I'm into canning my own foods which, I've been thinking about.
My advice, start with a 6q PC and then move up. You can sell a 6q PC on ebay for about what you paid for it assuming you didn't buy it new, but hell even the new ones are cheap. So you can always upgrade and recoup your money.
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funguy



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Quote:
hazyhorse said: as far as your agar goes... dunno what the dark spot is but i assume it has something to do with the water from the syringe. give it some time. spores take awhile to germinate & get going. the antibiotics may have something to do with it but i’m not sure. you should see some mycelium eventually. if this syringe is as dirty as it seems, you’re gonna get plenty of experience w agar (;
Hoping something will grow, maybe a transfer to non antibiotic agar once it does would make it grow faster. All this talk of contamination has me thinking I should just make some agar at work since we have a biosafety cabinet. Not in the mood to get fired tho
Quote:
fragrantdiscipline said:
I have a 6 quart pressure cooker. It serves my needs just fine. It's a good starting point and they can be had for cheap. It's definitely enough unless you need to sterilize a ton of stuff.
My advice, start with a 6q PC and then move up. You can sell a 6q PC on ebay for about what you paid for it assuming you didn't buy it new, but hell even the new ones are cheap. So you can always upgrade and recoup your money.
That's a good take on it, I'll see about getting a smaller one. Thanks
-------------------- uh oh stinky
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hazyhorse
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Re: Purple mold? [Re: toads]
#26481299 - 02/12/20 01:13 AM (3 years, 11 months ago) |
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maybe!! tbh i think the antibiotics are a little overkill. you take care of contamination with transfers to clean agar plates. lmao yeah i wouldn’t risk your job but... that would be clutch lol ;p
if you use a 6qt, remember to think about using pints instead of quarts. using quart jars over pints doubles my spawn making capabilities
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funguy



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Quote:
hazyhorse said: maybe!! tbh i think the antibiotics are a little overkill. you take care of contamination with transfers to clean agar plates. lmao yeah i wouldn’t risk your job but... that would be clutch lol ;p
if you use a 6qt, remember to think about using pints instead of quarts. using quart jars over pints doubles my spawn making capabilities
Aw hey it's what I had easy access to though I think it backfired cause I still don't see any growth after a full week. idk I was thinking I'd try bags or something since they hold more than my jars would. Wishful thinking at this point cause I'm about to not have any spores at all D:
-------------------- uh oh stinky
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hazyhorse
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Re: Purple mold? [Re: toads]
#26484028 - 02/13/20 04:28 PM (3 years, 11 months ago) |
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hahaha i definitely feel that. i wouldn't toss the plates though, spores might still germinate. if you don't have many spores left you might want to consider making a liquid culture with what you have left. it'll probably contaminate, but you should get mycelium to work with that way. if you have agar it's super easy to clean up
bags would probably be a good idea! i don't know much about bags personally but i'm sure it would be a step up from jars when using a smaller PC
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funguy



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Quote:
hazyhorse said: hahaha i definitely feel that. i wouldn't toss the plates though, spores might still germinate.
I think something is growing on it, but it's growing SO slowly. Its been like two weeks and you can barely see it. How do I know if I'm even growing mycelium at this point and not some super virus that will kill us all
-------------------- uh oh stinky
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hazyhorse
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Re: Purple mold? [Re: toads]
#26493583 - 02/19/20 03:10 PM (3 years, 11 months ago) |
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hmm, that does look odd. mycelium is typically white & kinda fluffy. maybe someone with more experience can tell you what you have going on
hopefully you aren't creating any super viruses... we already have enough going on as it is lol
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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