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toads
funguy



Registered: 01/29/20
Posts: 29
Loc: The Janitor's Closet
Last seen: 3 years, 1 month
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Re: Purple mold? [Re: Maylink]
#26479421 - 02/10/20 11:34 PM (3 years, 11 months ago) |
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Maylink said: RR’s signature line:
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"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Think of every failure as an opportunity to learn? And when you have success, it will be that much greater 
Will do! I feel better having advice from people here. Tysm 
and nope. still no growth on antibiotic agar. May try making my own agar without antibiotics next, if nothing happens.
-------------------- uh oh stinky
Edited by toads (02/11/20 12:20 AM)
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hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
Last seen: 13 hours, 9 minutes
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Re: Purple mold? [Re: toads]
#26479508 - 02/11/20 02:47 AM (3 years, 11 months ago) |
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LOL i feel that so hard, i hate using syringes. i always fuck them up. swear to god i used like 4cc on one of my first PF jars on accident lol
don’t sweat it!! my personal philosophy is it works when you need it to (of course, good technique & some experience go a long way in making that smoother ;D). you’ll get it
as far as your agar goes... dunno what the dark spot is but i assume it has something to do with the water from the syringe. give it some time. spores take awhile to germinate & get going. the antibiotics may have something to do with it but i’m not sure. you should see some mycelium eventually. if this syringe is as dirty as it seems, you’re gonna get plenty of experience w agar (;
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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Edited by hazyhorse (02/11/20 02:48 AM)
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fragrantdiscipline
LostWanderer


Registered: 01/02/20
Posts: 32
Last seen: 3 years, 2 months
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I sat them in boiling water for more than an hour
Boiling will kill most things. It won't kill everything. A pressure cooker is necessary to sterilize.
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Do you have any pressure cookers you recommend
I have a 6 quart pressure cooker. It serves my needs just fine. It's a good starting point and they can be had for cheap. It's definitely enough unless you need to sterilize a ton of stuff. I don't foresee needing something like a 23 quart pressure cooker unless I'm into canning my own foods which, I've been thinking about.
My advice, start with a 6q PC and then move up. You can sell a 6q PC on ebay for about what you paid for it assuming you didn't buy it new, but hell even the new ones are cheap. So you can always upgrade and recoup your money.
-------------------- Desperately in need of some stranger's hand in a desperate land.
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toads
funguy



Registered: 01/29/20
Posts: 29
Loc: The Janitor's Closet
Last seen: 3 years, 1 month
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hazyhorse said: as far as your agar goes... dunno what the dark spot is but i assume it has something to do with the water from the syringe. give it some time. spores take awhile to germinate & get going. the antibiotics may have something to do with it but i’m not sure. you should see some mycelium eventually. if this syringe is as dirty as it seems, you’re gonna get plenty of experience w agar (;
Hoping something will grow, maybe a transfer to non antibiotic agar once it does would make it grow faster. All this talk of contamination has me thinking I should just make some agar at work since we have a biosafety cabinet. Not in the mood to get fired tho
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fragrantdiscipline said:
I have a 6 quart pressure cooker. It serves my needs just fine. It's a good starting point and they can be had for cheap. It's definitely enough unless you need to sterilize a ton of stuff.
My advice, start with a 6q PC and then move up. You can sell a 6q PC on ebay for about what you paid for it assuming you didn't buy it new, but hell even the new ones are cheap. So you can always upgrade and recoup your money.
That's a good take on it, I'll see about getting a smaller one. Thanks
-------------------- uh oh stinky
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hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
Last seen: 13 hours, 9 minutes
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Re: Purple mold? [Re: toads]
#26481299 - 02/12/20 01:13 AM (3 years, 11 months ago) |
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maybe!! tbh i think the antibiotics are a little overkill. you take care of contamination with transfers to clean agar plates. lmao yeah i wouldn’t risk your job but... that would be clutch lol ;p
if you use a 6qt, remember to think about using pints instead of quarts. using quart jars over pints doubles my spawn making capabilities
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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toads
funguy



Registered: 01/29/20
Posts: 29
Loc: The Janitor's Closet
Last seen: 3 years, 1 month
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hazyhorse said: maybe!! tbh i think the antibiotics are a little overkill. you take care of contamination with transfers to clean agar plates. lmao yeah i wouldn’t risk your job but... that would be clutch lol ;p
if you use a 6qt, remember to think about using pints instead of quarts. using quart jars over pints doubles my spawn making capabilities
Aw hey it's what I had easy access to though I think it backfired cause I still don't see any growth after a full week. idk I was thinking I'd try bags or something since they hold more than my jars would. Wishful thinking at this point cause I'm about to not have any spores at all D:
-------------------- uh oh stinky
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hazyhorse
scoobin



Registered: 03/19/19
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Re: Purple mold? [Re: toads]
#26484028 - 02/13/20 04:28 PM (3 years, 11 months ago) |
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hahaha i definitely feel that. i wouldn't toss the plates though, spores might still germinate. if you don't have many spores left you might want to consider making a liquid culture with what you have left. it'll probably contaminate, but you should get mycelium to work with that way. if you have agar it's super easy to clean up
bags would probably be a good idea! i don't know much about bags personally but i'm sure it would be a step up from jars when using a smaller PC
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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toads
funguy



Registered: 01/29/20
Posts: 29
Loc: The Janitor's Closet
Last seen: 3 years, 1 month
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hazyhorse said: hahaha i definitely feel that. i wouldn't toss the plates though, spores might still germinate.
I think something is growing on it, but it's growing SO slowly. Its been like two weeks and you can barely see it. How do I know if I'm even growing mycelium at this point and not some super virus that will kill us all
-------------------- uh oh stinky
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hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
Last seen: 13 hours, 9 minutes
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Re: Purple mold? [Re: toads]
#26493583 - 02/19/20 03:10 PM (3 years, 11 months ago) |
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hmm, that does look odd. mycelium is typically white & kinda fluffy. maybe someone with more experience can tell you what you have going on
hopefully you aren't creating any super viruses... we already have enough going on as it is lol
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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