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Raleigh69
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Is this normal????? 1
#26469144 - 02/04/20 07:44 PM (3 years, 11 months ago) |
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3rd transfer, 2 days ago, no clue where the growth on the right came from .
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unibrowscowl
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Re: Is this normal????? [Re: Raleigh69] 1
#26469160 - 02/04/20 07:58 PM (3 years, 11 months ago) |
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Looks like myc to me. 
Maybe a small amount caught on your tool and fell off onto the side and started growing? Life finds a way.
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LadysKnight
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Re: Is this normal????? [Re: Raleigh69]
#26469163 - 02/04/20 08:01 PM (3 years, 11 months ago) |
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Yes it happens. Both look like mushroom mycelium. The one in the center looks like it has a ring of clearish mycelium around it, IME this is mold. I'd take transfers from the accidental culture on the right (unless it too has a semi-invisible halo)
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Raleigh69
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Just saying, I haven't seen growth like that in 10 days......That pic is 2 days after transfer.
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LadysKnight
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Re: Is this normal????? [Re: Raleigh69] 1
#26469219 - 02/04/20 08:39 PM (3 years, 11 months ago) |
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Sometimes it reaches out in front of the culture the whole time, looking like it's the leading edge, sometimes it's overtaken by the mushroom mycelium and is only seen early. I'd go with the one on the right.
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WeavieWonder
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Re: Is this normal????? [Re: Raleigh69]
#26469494 - 02/05/20 12:10 AM (3 years, 11 months ago) |
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Quote:
Raleigh69 said: Just saying, I haven't seen growth like that in 10 days......That pic is 2 days after transfer.
Transfer again asap. That shit on the right is mold.
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sporecap
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Quote:
WeavieWonder said:
Quote:
Raleigh69 said: Just saying, I haven't seen growth like that in 10 days......That pic is 2 days after transfer.
Transfer again asap. That shit on the right is mold.
 This amount of growth in the center in 2 days is normal for cubensis, but the stuff on the right is way to fast, typical for mold. You can also see some rhizo mycelium forming in the center, which is completely absent on the right.
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gizmo1



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Quote:
LadysKnight said: Yes it happens. Both look like mushroom mycelium. The one in the center looks like it has a ring of clearish mycelium around it, IME this is mold. I'd take transfers from the accidental culture on the right (unless it too has a semi-invisible halo)
You're saying that a clear leading edge means mold?
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LogicaL Chaos
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Re: Is this normal????? [Re: Raleigh69]
#26469594 - 02/05/20 02:17 AM (3 years, 11 months ago) |
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They both look like mycelium to me
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Bph
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Mold. 2 days and not in the place where you transfered too? Bet that shit turns grey on grains.
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LadysKnight
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Re: Is this normal????? [Re: gizmo1]
#26469645 - 02/05/20 04:14 AM (3 years, 11 months ago) |
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Quote:
Raleigh69 said: Just saying, I haven't seen growth like that in 10 days......That pic is 2 days after transfer.
Missed this. Guess I was pretty high last night. If only 2 days, likely mold on RT.
Quote:
gizmo1 said:
Quote:
LadysKnight said: Yes it happens. Both look like mushroom mycelium. The one in the center looks like it has a ring of clearish mycelium around it, IME this is mold. I'd take transfers from the accidental culture on the right (unless it too has a semi-invisible halo)
You're saying that a clear leading edge means mold?
Yes. It really is.
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LogicaL Chaos
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Re: Is this normal????? [Re: Bph]
#26469646 - 02/05/20 04:18 AM (3 years, 11 months ago) |
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The right spot does have a slight grey tint to it.....
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Bph
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I'm on a t4 of that same mold is the only reason I'm so sure. Mine dosent grow that fast but it looks just like that. Lossing hope that there is cube myc involved at all lol.
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Bph
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Re: Is this normal????? [Re: Bph]
#26469657 - 02/05/20 04:49 AM (3 years, 11 months ago) |
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Like this..
I can't figure out how you guys get good pics of cultures. But your pic looks like mine dose in real life. My pic looks like nothing. But I'm pretty sure they are both fucked lol.
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Raleigh69
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Re: Is this normal????? [Re: Bph]
#26470053 - 02/05/20 10:43 AM (3 years, 11 months ago) |
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Wow had no idea mold can so closely resemble mycelium. So how can we be sure? Thought the whole purpose of agar was to identify contams. Will it change color?
-------------------- Take the path, of most resistance
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Raccoon
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Re: Is this normal????? [Re: Raleigh69]
#26470102 - 02/05/20 11:12 AM (3 years, 11 months ago) |
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I'd transfer both, Just to see what happens and cover your bases. But transfer the center one first. Halos are common from my limited experience... To be sure, microscopes can help. And also waiting for it to change color when it sporulates like you said. After the transfer, keep the plate indefinitely (a month or two) to see what happens. Man, those both really do look like cube myc to me
-------------------- First Grow
Edited by Raccoon (02/05/20 11:13 AM)
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Raccoon
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Re: Is this normal????? [Re: Bph]
#26470108 - 02/05/20 11:18 AM (3 years, 11 months ago) |
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Quote:
Bph said: Like this..
I can't figure out how you guys get good pics of cultures. But your pic looks like mine dose in real life. My pic looks like nothing. But I'm pretty sure they are both fucked lol.
Yours looks different to me. More fine strands, but dense. Yours looks more questionable to me, but still hopeful... Let the previous generation plates go for awhile and see if they pin. Keep us updated, this is good stuff.
-------------------- First Grow
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Raleigh69
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Re: Is this normal????? [Re: Raccoon]
#26470178 - 02/05/20 12:00 PM (3 years, 11 months ago) |
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Just spotted this
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Psychgro
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Re: Is this normal????? [Re: Raleigh69]
#26470218 - 02/05/20 12:27 PM (3 years, 11 months ago) |
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What variety is this? Growth looks like lions mane or other wood lover. The green stuff is bad, obviously
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Raleigh69
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Re: Is this normal????? [Re: Psychgro]
#26470223 - 02/05/20 12:29 PM (3 years, 11 months ago) |
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Taman Negara
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Raleigh69
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Re: Is this normal????? [Re: Raleigh69]
#26471752 - 02/06/20 09:39 AM (3 years, 11 months ago) |
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2 day transfer from the culture on the left. Didnt bother with one on right
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gizmo1



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Re: Is this normal????? [Re: Raleigh69]
#26472932 - 02/06/20 09:38 PM (3 years, 11 months ago) |
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Doesn't look good at all.
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Whiterabbitfarms
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Re: Is this normal????? [Re: gizmo1]
#26473802 - 02/07/20 11:47 AM (3 years, 11 months ago) |
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Looks good to me man. I typically see that invisible line in early germination
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Raleigh69
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Re: Is this normal????? [Re: Raleigh69]
#26475029 - 02/08/20 07:05 AM (3 years, 11 months ago) |
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Sorry to keep posting,but with so many opposite opinions, it sounds like we might all be able to learn something from this one. This is the 2nd transfer of same culture
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LadysKnight
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Re: Is this normal????? [Re: Raleigh69]
#26475060 - 02/08/20 07:37 AM (3 years, 11 months ago) |
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That clear margin around the edge is still mold. Nice pic of that sneaky bastard. For science you should try to transfer it.
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Raccoon
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Here is a very informative thread about a clear margin: Read this post until the end. This growth turned out to be mold (with pictures). However, I've experienced clear margins described here which turned out to be cube myc. In that post I talk about the previous thread incorrectly (it was still an ongoing thread at the time and I misread the post). From these two examples I think you can compare what acceptable clear margins are and unacceptable. In looking at your plates after reading these posts, your growth looks more like a contam to me... But there is still some uncertainty there. If it changes color, we'll know. If it doesn't, then we will still be uncertain... Good luck!
-------------------- First Grow
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LadysKnight
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Re: Is this normal????? [Re: Raccoon]
#26475852 - 02/08/20 05:28 PM (3 years, 11 months ago) |
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Quote:
Raccoon said: Here is a very informative thread about a clear margin: Read this post until the end. This growth turned out to be mold (with pictures). However, I've experienced clear margins described here which turned out to be cube myc. In that post I talk about the previous thread incorrectly (it was still an ongoing thread at the time and I misread the post). From these two examples I think you can compare what acceptable clear margins are and unacceptable. In looking at your plates after reading these posts, your growth looks more like a contam to me... But there is still some uncertainty there. If it changes color, we'll know. If it doesn't, then we will still be uncertain... Good luck!
The clear margin pictured in this post and the one in your second link that turned out to be cube myc are quite a bit different. Yours was more milky/fuzzy. The one pictured in the last photo in this post is shiny/clear/glassy. It can only be seen if the light is shining at the proper angle, and cannot be seen when backlit. This one is mold, I know it well because it's kicking my ass. Also I have it isolated (accidentally) as a satellite contam where the transfer touched off-center when being placed on the dish. It is its own beautiful little glassy culture without the cube myc overlay. I don't know it's effects on cube myc, because I'm trying not to feed it any grain and find out.
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Raccoon
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I think you're right, LadysKnight. Do you have pics of your clear margin contamination? That and the isolated version? Posting it here may tie this thread up nicely.
-------------------- First Grow
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LadysKnight
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Re: Is this normal????? [Re: Raccoon]
#26476136 - 02/08/20 08:55 PM (3 years, 11 months ago) |
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I really wish I could take decent pics, especially of that clear mold, I've tried, I just don't have that ability. I wore out the search trying to figure it out, but opinions seem to have always been mixed, even amongst TCs. I'm just surprised to finally see a good picture of it, like trying to photograph a ghost. I am going to keep trying and to further isolate/identify/photograph it. Keep fighting the good fight.
Afterthought: The likelyhood of seeing a contam like this through anything but a perfectly clear petri (glass or plastic) is nill.
Edited by LadysKnight (02/08/20 09:32 PM)
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Nichrome
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The clear margin is not mold. The snotty stuff like that is a good sign. Healthy transfers so far. Here are some close up shots of rhizo tips emerging from snotty transparent margins.

from this plate here...

Which turned into these...

Good luck and keep on truckin'.
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
Edited by Nichrome (02/08/20 09:40 PM)
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LadysKnight
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Re: Is this normal????? [Re: Nichrome]
#26476187 - 02/08/20 09:47 PM (3 years, 11 months ago) |
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Hey, nice pics, but that's definitely something different. While sorta glassy, it's also very rhizomic. Looks much more like mushroom myc, even though clear. I'm sure there are many variations of clear margins, some benign, some mold. I see I'm just getting to know one of them.
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WeavieWonder
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Re: Is this normal????? [Re: Raleigh69]
#26476388 - 02/09/20 03:40 AM (3 years, 11 months ago) |
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Quote:
Raleigh69 said: Sorry to keep posting,but with so many opposite opinions, it sounds like we might all be able to learn something from this one. This is the 2nd transfer of same culture

Imo looks like.mold.
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Nichrome
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Not mold.
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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WeavieWonder
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Re: Is this normal????? [Re: Nichrome]
#26476793 - 02/09/20 10:59 AM (3 years, 11 months ago) |
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Th3 struggle continues.
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mushboy
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Re: Is this normal????? [Re: Nichrome]
#26476807 - 02/09/20 11:11 AM (3 years, 11 months ago) |
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Quote:
Nichrome said: Not mold. 
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Raccoon
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Quote:
WeavieWonder said: Th3 struggle continues.
Haha
OP, it looks like you need to fruit this growth so we can find out the truth. Thanks for sharing, your pictures are awesome.
-------------------- First Grow
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LadysKnight
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Re: Is this normal????? [Re: Raccoon]
#26477219 - 02/09/20 04:37 PM (3 years, 11 months ago) |
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Quote:
Raccoon said:
Quote:
WeavieWonder said: Th3 struggle continues.
Haha
OP, it looks like you need to fruit this growth so we can find out the truth. Thanks for sharing, your pictures are awesome.
Word. Great pics. I say the main culture is mush myc, the sexy clear halo around it is mold.
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Raleigh69
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Today
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Sockadin



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Re: Is this normal????? [Re: Raleigh69]
#26478870 - 02/10/20 04:17 PM (3 years, 11 months ago) |
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I see some mold.
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Nichrome
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Re: Is this normal????? [Re: Sockadin]
#26479027 - 02/10/20 05:58 PM (3 years, 11 months ago) |
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Definitely mold in there. You could save the culture still at this point if you transfer a few pieces of the middle colony to new plates. I've cleaned up worse lots of times. Wild prints are often riddled with other critters.
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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Sockadin



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Re: Is this normal????? [Re: Nichrome]
#26479199 - 02/10/20 07:51 PM (3 years, 11 months ago) |
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Because your mold has tinted to a green color the chances of it spreading those spores are higher. This is the moment if I did transfer it would be in a SAB instead of a FH.
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Raleigh69
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Re: Is this normal????? [Re: Sockadin] 1
#26483235 - 02/13/20 08:04 AM (3 years, 11 months ago) |
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-------------------- Take the path, of most resistance
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Nichrome
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Re: Is this normal????? [Re: Raleigh69]
#26483851 - 02/13/20 02:39 PM (3 years, 11 months ago) |
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Really nice so far. Keep it up and you've got a winner (or two or three from that plate with the bear face). Good job.
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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