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OfflineRaleigh69
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Re: Is this normal????? [Re: Raleigh69]
    #26471752 - 02/06/20 09:39 AM (3 years, 11 months ago)

2 day transfer from the culture on the left. Didnt bother with one on right


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Invisiblegizmo1
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Re: Is this normal????? [Re: Raleigh69]
    #26472932 - 02/06/20 09:38 PM (3 years, 11 months ago)

Doesn't look good at all.


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OfflineWhiterabbitfarms
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Re: Is this normal????? [Re: gizmo1]
    #26473802 - 02/07/20 11:47 AM (3 years, 11 months ago)

Looks good to me man. I typically see that invisible line in early germination


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OfflineRaleigh69
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Re: Is this normal????? [Re: Raleigh69]
    #26475029 - 02/08/20 07:05 AM (3 years, 11 months ago)

Sorry to keep posting,but with so many opposite opinions,  it sounds like we might all be able to learn something from this one. This is the 2nd transfer of same culture



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InvisibleLadysKnight
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Re: Is this normal????? [Re: Raleigh69]
    #26475060 - 02/08/20 07:37 AM (3 years, 11 months ago)

That clear margin around the edge is still mold.
Nice pic of that sneaky bastard.
For science you should try to transfer it.


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OfflineRaccoon
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Re: Is this normal????? [Re: LadysKnight]
    #26475169 - 02/08/20 09:10 AM (3 years, 11 months ago)

Here is a very informative thread about a clear margin: Read this post until the end. This growth turned out to be mold (with pictures). However, I've experienced clear margins described here which turned out to be cube myc. In that post I talk about the previous thread incorrectly (it was still an ongoing thread at the time and I misread the post). From these two examples I think you can compare what acceptable clear margins are and unacceptable. In looking at your plates after reading these posts, your growth looks more like a contam to me... But there is still some uncertainty there. If it changes color, we'll know. If it doesn't, then we will still be uncertain... Good luck!


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InvisibleLadysKnight
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Re: Is this normal????? [Re: Raccoon]
    #26475852 - 02/08/20 05:28 PM (3 years, 11 months ago)

Quote:

Raccoon said:
Here is a very informative thread about a clear margin: Read this post until the end. This growth turned out to be mold (with pictures). However, I've experienced clear margins described here which turned out to be cube myc. In that post I talk about the previous thread incorrectly (it was still an ongoing thread at the time and I misread the post). From these two examples I think you can compare what acceptable clear margins are and unacceptable. In looking at your plates after reading these posts, your growth looks more like a contam to me... But there is still some uncertainty there. If it changes color, we'll know. If it doesn't, then we will still be uncertain... Good luck!





The clear margin pictured in this post and the one in your second link that turned out to be cube myc are quite a bit different. Yours was more milky/fuzzy. The one pictured in the last photo in this post is shiny/clear/glassy. It can only be seen if the light is shining at the proper angle, and cannot be seen when backlit. This one is mold, I know it well because it's kicking my ass. Also I have it isolated (accidentally) as a satellite contam where the transfer touched off-center when being placed on the dish. It is its own beautiful little glassy culture without the cube myc overlay. I don't know it's effects on cube myc, because I'm trying not to feed it any grain and find out.


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OfflineRaccoon
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Re: Is this normal????? [Re: LadysKnight]
    #26476076 - 02/08/20 07:58 PM (3 years, 11 months ago)

I think you're right, LadysKnight. Do you have pics of your clear margin contamination? That and the isolated version? Posting it here may tie this thread up nicely.


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InvisibleLadysKnight
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Re: Is this normal????? [Re: Raccoon]
    #26476136 - 02/08/20 08:55 PM (3 years, 11 months ago)

I really wish I could take decent pics, especially of that clear mold, I've tried, I just don't have that ability. I wore out the search trying to figure it out, but opinions seem to have always been mixed, even amongst TCs. I'm just surprised to finally see a good picture of it, like trying to photograph a ghost. I am going to keep trying and to further isolate/identify/photograph it. Keep fighting the good fight.

Afterthought: The likelyhood of seeing a contam like this through anything but a perfectly clear petri (glass or plastic) is nill.


Edited by LadysKnight (02/08/20 09:32 PM)


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OfflineNichrome
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Re: Is this normal????? [Re: LadysKnight]
    #26476178 - 02/08/20 09:38 PM (3 years, 11 months ago)

The clear margin is not mold. The snotty stuff like that is a good sign. Healthy transfers so far. Here are some close up shots of rhizo tips emerging from snotty transparent margins.



from this plate here...


Which turned into these...



Good luck and keep on truckin'.


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Better to be deprived of food for three days, than tea for one.


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Edited by Nichrome (02/08/20 09:40 PM)


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InvisibleLadysKnight
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Re: Is this normal????? [Re: Nichrome]
    #26476187 - 02/08/20 09:47 PM (3 years, 11 months ago)

Hey, nice pics, but that's definitely something different. While sorta glassy, it's also very rhizomic. Looks much more like mushroom myc, even though clear. I'm sure there are many variations of clear margins, some benign, some mold. I see I'm just getting to know one of them.


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OfflineWeavieWonder
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Re: Is this normal????? [Re: Raleigh69]
    #26476388 - 02/09/20 03:40 AM (3 years, 11 months ago)

Quote:

Raleigh69 said:
Sorry to keep posting,but with so many opposite opinions,  it sounds like we might all be able to learn something from this one. This is the 2nd transfer of same culture






Imo looks like.mold.


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OfflineNichrome
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Re: Is this normal????? [Re: WeavieWonder]
    #26476607 - 02/09/20 09:00 AM (3 years, 11 months ago)

Not mold. :heart:


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OfflineWeavieWonder
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Re: Is this normal????? [Re: Nichrome]
    #26476793 - 02/09/20 10:59 AM (3 years, 11 months ago)

Th3 struggle continues.:lol:


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InvisiblemushboyMDiscord
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Re: Is this normal????? [Re: Nichrome]
    #26476807 - 02/09/20 11:11 AM (3 years, 11 months ago)

Quote:

Nichrome said:
Not mold. :heart:




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OfflineRaccoon
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Re: Is this normal????? [Re: WeavieWonder]
    #26476898 - 02/09/20 12:27 PM (3 years, 11 months ago)

Quote:

WeavieWonder said:
Th3 struggle continues.:lol:



Haha  :lol:

OP, it looks like you need to fruit this growth so we can find out the truth. Thanks for sharing, your pictures are awesome.


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InvisibleLadysKnight
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Re: Is this normal????? [Re: Raccoon]
    #26477219 - 02/09/20 04:37 PM (3 years, 11 months ago)

Quote:

Raccoon said:
Quote:

WeavieWonder said:
Th3 struggle continues.:lol:



Haha  :lol:

OP, it looks like you need to fruit this growth so we can find out the truth. Thanks for sharing, your pictures are awesome.




Word. Great pics. I say the main culture is mush myc, the sexy clear halo around it is mold.


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OfflineRaleigh69
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Re: Is this normal????? [Re: LadysKnight]
    #26478799 - 02/10/20 03:43 PM (3 years, 11 months ago)

Today


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OfflineSockadin
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Re: Is this normal????? [Re: Raleigh69]
    #26478870 - 02/10/20 04:17 PM (3 years, 11 months ago)

I see some mold.


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OfflineNichrome
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Re: Is this normal????? [Re: Sockadin]
    #26479027 - 02/10/20 05:58 PM (3 years, 11 months ago)

Definitely mold in there. You could save the culture still at this point if you transfer a few pieces of the middle colony to new plates. I've cleaned up worse lots of times. Wild prints are often riddled with other critters.


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Better to be deprived of food for three days, than tea for one.


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