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Offlineslimeysnail
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Registered: 07/01/19
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First time isolating genetics. Where to take transfers from?
    #26468470 - 02/04/20 12:44 PM (4 years, 13 days ago)

Hey all, I recently got into agar and am trying to isolate some strong genetics. This is one week post first transfer from a MS plate. Where should I take transfers from? From what I understand, you want to isolate rizomorphic growth (obviously), and you want to take it from the leading edge?



Edited by slimeysnail (02/04/20 12:44 PM)


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OfflineKmacmo
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Registered: 08/14/19
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Re: First time isolating genetics. Where to take transfers from? [Re: slimeysnail]
    #26468498 - 02/04/20 01:01 PM (4 years, 13 days ago)

The top half looks nice and rhizo, I'd take from 11.30 and 3 o'clock.

Looks great for first attempt tho :smile:


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Offlinenarmaduke
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Re: First time isolating genetics. Where to take transfers from? [Re: slimeysnail]
    #26468501 - 02/04/20 01:03 PM (4 years, 13 days ago)

12 o’clock on that really dense part, but basically from 10 to 3ish looks nice. yes get it from the leading edge and make sure it’s no larger than a grain of rice!


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InvisibleSpunkyMonkey88
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Re: First time isolating genetics. Where to take transfers from? [Re: narmaduke]
    #26468526 - 02/04/20 01:23 PM (4 years, 13 days ago)

I would definitely take this segment..



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Offlineslimeysnail
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Registered: 07/01/19
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Last seen: 3 years, 4 months
Re: First time isolating genetics. Where to take transfers from? [Re: SpunkyMonkey88]
    #26468629 - 02/04/20 02:30 PM (4 years, 13 days ago)

Much appreciated :smile:
Quote:

Kmacmo said:
The top half looks nice and rhizo, I'd take from 11.30 and 3 o'clock.

Looks great for first attempt tho :smile:




much appreciated! :smile:

Quote:

narmaduke said:
12 o’clock on that really dense part, but basically from 10 to 3ish looks nice. yes get it from the leading edge and make sure it’s no larger than a grain of rice!




got it, thank you for your input!

Quote:

SpunkyMonkey88 said:
I would definitely take this segment..






Will definitely make that one of the cuts. Thank you!


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