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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26464322 - 02/01/20 08:34 PM (4 years, 15 days ago)

You can save them for years and it wont make much difference. Ive stored LI syringes for 7 years and had success growing them out. I dont see any difference in speed. But i also keep them in the fridge.


7 year old LI


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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26464328 - 02/01/20 08:42 PM (4 years, 15 days ago)

wow. 7 effin years.

Cool.... I'll do room temp for a few days, see what happens.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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OnlineNichrome
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26464366 - 02/01/20 09:29 PM (4 years, 15 days ago)

Quote:

tedoro said:


More of my plates after a few more days.





Burma?


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Re: EAT LIQUID INOCULATES™ [Re: Nichrome]
    #26464413 - 02/01/20 10:24 PM (4 years, 15 days ago)

Quote:

Nichrome said:
Quote:

tedoro said:


More of my plates after a few more days.





Burma?




3rd transfer of MS golden teacher on very soft MEA plates that I spiked with Karo.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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OnlineNichrome
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26464438 - 02/01/20 11:01 PM (4 years, 15 days ago)

Nice! I've got a burma that gets all pizza pie looking like those. None of my GT have acted like that but GT has been around forever so I'm sure there are various sets of lineage at this point. Looks beautiful!

Does your GT grow very many pebbles or blobs when it pins?


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Re: EAT LIQUID INOCULATES™ [Re: Nichrome]
    #26464463 - 02/01/20 11:26 PM (4 years, 14 days ago)

Quote:

Nichrome said:
Nice! I've got a burma that gets all pizza pie looking like those. None of my GT have acted like that but GT has been around forever so I'm sure there are various sets of lineage at this point. Looks beautiful!

Does your GT grow very many pebbles or blobs when it pins?




not sure what a pebble is, but I can imagine. nope... no blobs or none traditional mushrooms.


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Clean spawn thread | Put a thermometer on your PC


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OnlineNichrome
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26464483 - 02/01/20 11:49 PM (4 years, 14 days ago)

Here are some GT pinsets with the "pebbles" I referred. The ones that do this always kick ass.



This was LC to grain to bulk.


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Better to be deprived of food for three days, than tea for one.


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Re: EAT LIQUID INOCULATES™ [Re: Nichrome]
    #26464647 - 02/02/20 04:53 AM (4 years, 14 days ago)

Like white blobs on your plates? Do they ever mature into pins if you let them go?


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Edited by eatyualive (02/02/20 05:33 AM)


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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26466701 - 02/03/20 11:49 AM (4 years, 13 days ago)



Day seven. How does this look? These .5 bags dry out the top surface but they fully colonize, just takes an extra day. I could move to .2 bags, I think I can easily pre-seal them too, now that I get pre-sealing.


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Edited by tedoro (02/03/20 12:05 PM)


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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26466826 - 02/03/20 01:33 PM (4 years, 13 days ago)

It’s the filter. Ive heard of several people using the .2 just for that same reason. Looks good almost there. Fairly close to 7-8 days. I’d say you found the right volume. Stick with that formula you just used unless you are searching for a little bit faster speed. What was your normal time for colonization in those bags?


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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26466842 - 02/03/20 01:46 PM (4 years, 13 days ago)

Crazy... something makes the red go away... maybe the mycelia is eating it? The plate is evenly thick and was all red. No uneven lighting to fade that side.



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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26466846 - 02/03/20 01:49 PM (4 years, 13 days ago)

Yes mycelia will colonize the agar all the way through and it will lose its color over time.


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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26466865 - 02/03/20 01:58 PM (4 years, 13 days ago)

Quote:

eatyualive said:
Yes mycelia will colonize the agar all the way through and it will lose its color over time.




Thats good news for people making LI. My transfers from these are looking better.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26467597 - 02/03/20 09:29 PM (4 years, 13 days ago)

Quote:

eatyualive said:
It’s the filter. Ive heard of several people using the .2 just for that same reason. Looks good almost there. Fairly close to 7-8 days. I’d say you found the right volume. Stick with that formula you just used unless you are searching for a little bit faster speed. What was your normal time for colonization in those bags?




I was happy with two weeks before, My plates were poured very thin.... and I didn't boil the shit out of the wheat berries, which seems to help.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26467642 - 02/03/20 09:54 PM (4 years, 13 days ago)

I keep all my grain on the dry side. Specifically for LI. Ive been working on a ton of gif videos of each step. I have them but I’m having issues uploading. I’ll update as soon as I can get it working. I got a video from above of the lid swap, unscrewing receiving jar lid, inoculation, unwrapping the foil lid.


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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26468079 - 02/04/20 08:20 AM (4 years, 12 days ago)

just dont make it so dry that the inside of the grain doesnt fully hydrate and contams your LI when you blend it up and expose the starchy insides:facepalm:    did that a bunch when I was first prepping milo.  didnt know I was ever underhydrating the grain until I tried blending some for LI.

0.2 pre-seal just fine.  I like the .5's cause u can smell clean or dirty spawn theough them a bit better.  0.5's dry out a bit more in the winter months tho


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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: TedsDead]
    #26470829 - 02/05/20 06:04 PM (4 years, 11 days ago)

All I do is let the grain strain until it’s dry with no moisture on the outside. Then pc an 1 hour 15 mins. I could get away with 1 hour and I did for years and years. Recently I started cooking for 15-30 extra but I see no difference in contam rate or anything.


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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26470860 - 02/05/20 06:25 PM (4 years, 11 days ago)

alright, Im growing out a  couple plates for an LI again.  you guys reelled  me in.


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: TedsDead] * 1
    #26477366 - 02/09/20 06:08 PM (4 years, 7 days ago)

I got some top view gifs of a few of the techniques described in the op. I’m currently updating step by step with gifs.




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Edited by eatyualive (02/09/20 07:08 PM)


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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26478294 - 02/10/20 10:15 AM (4 years, 6 days ago)

Cool gifs!! Thanks for doing that!



I let my LI sit out at room temp for 8 days. Didn't see a great deal of growth, maybe some? Hard to tell when it was mostly stuffed with LI already.

Leapoff clearly seen the next morning (20hrs) when photo was taken. My grains usually take 4 days to look like this. Pretty cool. A little odd that some of the agar pieces leapt, and others didn't. Maybe the agar that was colonized thoroughly were the ones to leap. This was an old control bag from months ago that smelled good, but was a little dry. So I don't think it will mind 90cc's of LI. I'll keep y'all posted.


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Edited by tedoro (02/10/20 12:13 PM)


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