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MckennaTek
Stranger

Registered: 02/03/20
Posts: 1
Last seen: 3 years, 9 months
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Petri Dishes
#26466316 - 02/03/20 06:06 AM (3 years, 11 months ago) |
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Hi, guys im new to Petri dishes. Could you give me some tips, please? maybe some things to avoid?
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FrankRhizo
Interdimensional Travel Agent

Registered: 11/28/17
Posts: 295
Loc: Subterranean
Last seen: 6 months, 3 days
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Really just practice. Pour a sleeve of them, see how it is for your situation (sab or flow). See if any containment from your pour. Do blank transfers to see how it all feels. It's a motor memory thing. You have to get the right temp or condensation will obstruct your view.
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PyeR2
Phungophiliac


Registered: 01/16/20
Posts: 26
Loc: Australia - NSW
Last seen: 8 days, 3 hours
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When growing out mycelium in petri dishes, should they be kept in the dark or is indoor light ok? or does it not really matter?
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Strainsfordaze

Registered: 05/10/18
Posts: 669
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Re: Petri Dishes [Re: PyeR2]
#26466416 - 02/03/20 08:08 AM (3 years, 11 months ago) |
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You want to pour the dishes around 40-50 degrees c to prevent condensation. Make sure you appropriately sterilize your scalpel every transfer. Patiences is key for this hobby. You’ll develop a feel for everything after the first couple times. Light is fine, no need to be kept in the dark.
Edited by Strainsfordaze (02/03/20 09:46 AM)
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InfiniteDreams


Registered: 10/25/19
Posts: 1,224
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Quote:
Strainsfordaze said: You want to pour the dishes around 140-150 degree F to prevent condensation.
Pouring at that temp guarantees condensation. You want to pour as cool as you can before it starts to gel up. Bod recommends 117 degrees F as optimal in his tek.
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,670
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Quote:
InfiniteDreams said:
Quote:
Strainsfordaze said: You want to pour the dishes around 140-150 degree F to prevent condensation.
Pouring at that temp guarantees condensation. You want to pour as cool as you can before it starts to gel up. Bod recommends 117 degrees F as optimal in his tek.
Or pour at any temp in open air, then sterilize them after. This is known as "no-pour".
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Strainsfordaze

Registered: 05/10/18
Posts: 669
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Lol I wasn’t thinking when writing that..burnt. Meant 40-50 degrees c at pour. Thanks for correction.
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InfiniteDreams


Registered: 10/25/19
Posts: 1,224
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Quote:
LadysKnight said: Or pour at any temp in open air, then sterilize them after. This is known as "no-pour".
That is another valid approach. But most people aren't using Petri dishes for that as the plastic wouldn't handle the PC.
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Robomouse
Stranger
Registered: 11/06/19
Posts: 1
Last seen: 3 years, 11 months
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Hello, brand new noob, first comment/post - my agar is pulling away from the sides of the Petri dish, they are in unzipped ziploc bags. Does this mean they are drying out? Should I zip the bags shut? They are currently living in a shoebox, heated with a lower tub full of water with an aquarium heater.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,273
Loc: where?
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Quote:
MckennaTek said: Hi, guys im new to Petri dishes. Could you give me some tips, please? maybe some things to avoid?
1.) make videos of yourself pouring petris and doing sterile work(inoculations ect..)
2.) post them on shroomery.
3.) listen to advice given for free to help you become a master shroom grower.
4.)
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,670
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Quote:
InfiniteDreams said:
Quote:
LadysKnight said: Or pour at any temp in open air, then sterilize them after. This is known as "no-pour".
That is another valid approach. But most people aren't using Petri dishes for that as the plastic wouldn't handle the PC.
Definitely depends on the dishes chosen. These are all valid noob considerations.
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,670
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Quote:
Robomouse said: Hello, brand new noob, first comment/post - my agar is pulling away from the sides of the Petri dish, they are in unzipped ziploc bags. Does this mean they are drying out? Should I zip the bags shut? They are currently living in a shoebox, heated with a lower tub full of water with an aquarium heater.
They should have been zipped shut when you put them in the bag, yes. Lose the heater.
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