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Offlinepsycho_nauticus
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1/2 cc left - enough for agar work?
    #26466283 - 02/03/20 05:16 AM (3 years, 11 months ago)

I have 1/2 cc left of B+, is that enough to do agar work and then inoculate jars? It would be my very first time doing any agar work ever and looking to grow a couple shoeboxes.


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OfflineOne of Us
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Re: 1/2 cc left - enough for agar work? [Re: psycho_nauticus]
    #26466284 - 02/03/20 05:19 AM (3 years, 11 months ago)

Should be plenty. You only need a drop or two


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OfflineFrankRhizo
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Re: 1/2 cc left - enough for agar work? [Re: One of Us]
    #26466292 - 02/03/20 05:37 AM (3 years, 11 months ago)

About 10 drops. So yes.


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OfflineMushkingMulah360
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Re: 1/2 cc left - enough for agar work? [Re: FrankRhizo]
    #26466324 - 02/03/20 06:22 AM (3 years, 11 months ago)

Yea that’s plenty! I find less is more when doing Ms syringe to agar, and try to make sure you get the tiniest drop directly center of the plate it’s easier to differentiate contams vs mycelium. Once you get some clean growth transfer out. Do that 3-4 times and you got yourself a nice clean plate for A2G, which you can just keep making transfers to new plates to expand as long as you always keep atleast one clean plate. This will pretty much mean you have unlimited supply of already germinated and alive mycelium making colonization much faster/cleaner in the future. Still pretty new to this just jumped into agar a few weeks ago, my only regret is not starting it when I first started! I would probably be drying my first flushes if I did 🤦🏻‍♂️🤷🏻‍♂️. This time around will be much different !!! Good luck brotha.


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