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Mr.Wizard
Harbinger of Hallucination



Registered: 01/20/20
Posts: 281
Last seen: 17 days, 15 hours
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Preparing for second transfers, wanting opinions on these plates, please.
#26465170 - 02/02/20 12:06 PM (4 years, 14 days ago) |
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These are some of my first transfers (ones in easy to see petris). I have some ideas on what's looking better, but my mycology eye hasn't developed much yet. Each set of 3 were taken from the same original germ plate.

Do y'all think I should continue isolating all of them, or pick the best of each variety?
I'm also interested in what any of you see that I may not. I think they are all looking OK, but I think RW1X3, and PE62X3 are looking the best.
Thank you for any replies! Go Chiefs!
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 3 months, 7 days
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Re: Preparing for second transfers, wanting opinions on these plates, please. [Re: Mr.Wizard]
#26465172 - 02/02/20 12:09 PM (4 years, 14 days ago) |
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I like RW 1 and Rw 3. You might let 3 grow out. Then take 1 of each side of RW1
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WeavieWonder
Time Travel Sucks



Registered: 08/18/16
Posts: 1,455
Loc: USA
Last seen: 1 month, 21 days
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Re: Preparing for second transfers, wanting opinions on these plates, please. [Re: Sockadin]
#26465218 - 02/02/20 12:43 PM (4 years, 14 days ago) |
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Of all the pe6, 2x3 looks the most promising. Let the other ones grow out. You never know.
Rw1x1 and rw1x2 look good. Rw1x3 looks like bacteria city.
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Mr.Wizard
Harbinger of Hallucination



Registered: 01/20/20
Posts: 281
Last seen: 17 days, 15 hours
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Re: Preparing for second transfers, wanting opinions on these plates, please. [Re: WeavieWonder]
#26465266 - 02/02/20 01:14 PM (4 years, 14 days ago) |
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Bacteria City, awesome! I haven't been able to 'see' any myself yet, and have been starting to get worried it's just that, I'm not seeing it.
Can you elaborate on what brought you to that conclusion? Here are more pictures (these plates were my first pours, and have some sediment, I have multiple that could be considered controls, and have not contam'd).
Here's more pictures of RW1X3.
EDIT: I also wanted to say that these are subject to 12on/12off 6500k light. 65 degrees when it's off, 75 degrees when it's on.
From bottom with light behind:

From to with light behind:

Close up of top w/out light behind:
Edited by Mr.Wizard (02/02/20 01:17 PM)
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 3 months, 7 days
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Re: Preparing for second transfers, wanting opinions on these plates, please. [Re: WeavieWonder]
#26465337 - 02/02/20 01:45 PM (4 years, 14 days ago) |
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Quote:
WeavieWonder said: Of all the pe6, 2x3 looks the most promising. Let the other ones grow out. You never know.
Rw1x1 and rw1x2 look good. Rw1x3 looks like bacteria city.
I don't see any bacteria in RW1x3
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WeavieWonder
Time Travel Sucks



Registered: 08/18/16
Posts: 1,455
Loc: USA
Last seen: 1 month, 21 days
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Re: Preparing for second transfers, wanting opinions on these plates, please. [Re: Sockadin]
#26465450 - 02/02/20 02:48 PM (4 years, 14 days ago) |
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Quote:
Sockadin said:
Quote:
WeavieWonder said: Of all the pe6, 2x3 looks the most promising. Let the other ones grow out. You never know.
Rw1x1 and rw1x2 look good. Rw1x3 looks like bacteria city.
I don't see any bacteria in RW1x3
After looking at the latest pics of rw1x3, I think you're right. More likely mold. Any time i see puffy, irradic growth like that at the epicenter, it usually means a contam of some sort is present. Ime, shedding a mold contam is damn near impossible. Op did the right thing and took multiple transfers from T0. Just work with the best looking one(s), and toss the rest. Rw1x3 gets my vote for the trash.
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 3 months, 7 days
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Re: Preparing for second transfers, wanting opinions on these plates, please. [Re: WeavieWonder]
#26465486 - 02/02/20 03:15 PM (4 years, 14 days ago) |
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I say keep it and test the difference of opinion. I think it looks great. But hell I'm no Trusted Cultivator so..
Maybe I need to work on getting my TC tag I been on here long enough.
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