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Offlineoxo9
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Registered: 11/26/19
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Panaeolus cyanescens. Progress with agar, Liquid Culture, and cakes.
    #26464613 - 02/02/20 03:39 AM (3 years, 11 months ago)

Hello, here is my progress with Panaeolus cyanescens.

My cakes are Brown Rice / Black Kow manure / Vermiculite (with water to approximate field capacity [in <5 minutes]. Boiled in a deep pan with a lid for 2 hours. inoculated on 1/22/2020.
   
        Cakes
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Agar was poured from a solution of 500 ml Bottled water, 10 g of Agar, 10 g of Mae, 5 g of Black Kow. The Black Kow is some sort of dirt/compost mixed with black cow manure. I made it as powdery as possible but when I mixed it in a liquid solution only 1/5 of it actually dissolved into the water while the rest remained at the bottom of the pot as dirt. I added approx. 2 g of gypsum. Inoculated with spore print 1/30/2020

    Agar
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LC
This LC is poured from a 500 ml solution with 10 g of confectionery sugar and 10 mg of malt extract. Boiled for 2 hours. The visible solutes fell out within a few hours of pouring so I believe it is just my solute over-saturating my solvent. Inoculated with spore prints on 1/30/2020

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OfflineTheApprentice
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Registered: 09/25/11
Posts: 4,727
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Re: Panaeolus cyanescens. Progress with agar, Liquid Culture, and cakes. [Re: oxo9]
    #26464816 - 02/02/20 08:15 AM (3 years, 11 months ago)

ok so... all those things are contaminated.

sorry. 

time to start over and be extra careful on your sterile technique. 

:didyouseethat:


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Offlinejcm4620
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Registered: 05/26/19
Posts: 6,700
Last seen: 1 year, 11 months
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Re: Panaeolus cyanescens. Progress with agar, Liquid Culture, and cakes. [Re: TheApprentice]
    #26464937 - 02/02/20 09:34 AM (3 years, 11 months ago)

yikes ya them r pretty bad


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PANAEOLUS FRUITING MADE SIMPLE



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OfflineMushkingMulah360
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Registered: 01/13/20
Posts: 184
Loc: Flag
Last seen: 3 years, 8 months
Re: Panaeolus cyanescens. Progress with agar, Liquid Culture, and cakes. [Re: oxo9]
    #26464953 - 02/02/20 09:41 AM (3 years, 11 months ago)

RESTART, I’d chalk this one up as a learning experience and keep it moving.


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OfflineSockadin
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Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Re: Panaeolus cyanescens. Progress with agar, Liquid Culture, and cakes. [Re: MushkingMulah360]
    #26464969 - 02/02/20 09:48 AM (3 years, 11 months ago)

In picture 3 of you agar jars. See that opake swirl at 6:00? That is a bacterial contamination, so I would rethink the sterilzation tek. But keep at it we are excited to see more!


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