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OfflineJoeyFatone
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Good growth in jar, or contams?
    #26463376 - 02/01/20 10:26 AM (3 years, 11 months ago)

Finally found the right place to post this. Sweet.

So I'm seeing what looks to be white 'grains'. Is that the good shit starting to form? Or contam? Neither?

Also, if you look within the red circle, it kinda looks like the white 'grains' are forming a 'clump'. That's normal, hey?

Super nervous about contams. First time doing this, which is probably why my worries are so high. It's probably day 3 or 4. If these aren't contams, that must mean I'm off to a good start, hey?

Thanks for bearing with a noob and his noob questions.

<trying to grab a photo hold on>


Edited by JoeyFatone (02/01/20 10:30 AM)


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OfflineJoeyFatone
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Re: Good growth in jar, or contams? [Re: JoeyFatone]
    #26463381 - 02/01/20 10:31 AM (3 years, 11 months ago)



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Re: Good growth in jar, or contams? [Re: JoeyFatone]
    #26463384 - 02/01/20 10:31 AM (3 years, 11 months ago)

Neither :shrug:


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OfflineJoeyFatone
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Re: Good growth in jar, or contams? [Re: bodhisatta]
    #26463389 - 02/01/20 10:33 AM (3 years, 11 months ago)

That's odd. The white "grains" weren't there before...


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Re: Good growth in jar, or contams? [Re: JoeyFatone]
    #26463400 - 02/01/20 10:40 AM (3 years, 11 months ago)

You're looking at them too much


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OfflineJoeyFatone
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Re: Good growth in jar, or contams? [Re: bodhisatta]
    #26463404 - 02/01/20 10:43 AM (3 years, 11 months ago)

I don't understand; they weren't there before, though.


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Re: Good growth in jar, or contams? [Re: JoeyFatone]
    #26463517 - 02/01/20 11:46 AM (3 years, 11 months ago)

They're all over the whole jar :shrug: thats your wet brf power


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OfflineJoeyFatone
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Re: Good growth in jar, or contams? [Re: bodhisatta]
    #26463529 - 02/01/20 11:57 AM (3 years, 11 months ago)

Okay, thanks!

But whatever it is, there's definitely more of it now. White salt-like spots appearing.And it doesn't look very wet at all. More showing up day-by-day. Is this a sign that it's starting to colonize? These definitely weren't there before, days ago. It's definitely something that happened after the jars were sitting, they weren't there already.


Edited by JoeyFatone (02/01/20 12:01 PM)


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Re: Good growth in jar, or contams? [Re: JoeyFatone] * 1
    #26463536 - 02/01/20 12:01 PM (3 years, 11 months ago)

Mycelium is a sign it's starting to colonize.

Your jars look 150% exactly like PF cakes before anything has happened


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OfflineJoeyFatone
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Re: Good growth in jar, or contams? [Re: bodhisatta]
    #26463593 - 02/01/20 12:28 PM (3 years, 11 months ago)

So if that is the case, is day 5 with no growth not a good sign?

The salt thing still kinda worries me, as there's more. It didn't begin this way.


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Offlinejbgtaa
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Re: Good growth in jar, or contams? [Re: JoeyFatone]
    #26463597 - 02/01/20 12:29 PM (3 years, 11 months ago)

How did u inoculate? If witha spore syringe or print, you may not see growth for up to 2 weeks.


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OfflineJoeyFatone
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Re: Good growth in jar, or contams? [Re: jbgtaa]
    #26463602 - 02/01/20 12:32 PM (3 years, 11 months ago)

syringe; SAB, sanitized with pc, took all the necessary precautions.


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Re: Good growth in jar, or contams? [Re: JoeyFatone]
    #26463625 - 02/01/20 12:52 PM (3 years, 11 months ago)

Spores have to germinate. That can take up to five days on its own, and germination happens at a microscopic level. It’ll take up to 2 weeks to see any truly visible growth.

That said whatever your looking at in your jars was probably there to start, and it’s just drying and shit is solidifying.

Doesn’t look like anything is there and looks like a well prepared PF jar. That’s it.


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Re: Good growth in jar, or contams? [Re: jbgtaa]
    #26463701 - 02/01/20 01:32 PM (3 years, 11 months ago)

Do you like to fish? Or perhaps play video games like Fortnight or CoD? Cause these next few days to weeks you should focus on anything but picking up and touching those jars. Even at the angle you are holding the jar in you picture you have tilted the dry vermiculite barrier and Everytime you touch them you run the risk of allowing contams into your process.


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Re: Good growth in jar, or contams? [Re: Sockadin]
    #26463775 - 02/01/20 02:18 PM (3 years, 11 months ago)

BRF is pretty contaminant resistant. That’s why no one puts filters on them. The verm barrier is for things like mold, which in OPs case might be a problem considering the extra water he added.


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OfflineJoeyFatone
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Re: Good growth in jar, or contams? [Re: jbgtaa]
    #26465051 - 02/02/20 10:31 AM (3 years, 11 months ago)

Thanks guys, I appreciate it!

Kinda worrying at this point.

It's day 6 or 7. Absolutely zero growth. I bought a syringe off of a very reputable thing.

And sock, already done with my challenges, so I'm fortnite'd out, mate. Haha!

When I look into the substrate, it doesn't look dry, and it doesn't look wet- like it looks like a perfect consistency.  But yeah. No growth yet and it's day 6 or 7, and I'm kind of worrying a bit.


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Re: Good growth in jar, or contams? [Re: JoeyFatone]
    #26465065 - 02/02/20 10:44 AM (3 years, 11 months ago)

I have had jars that took 2 weeks to see visable growth. I have had jars that never grew... It's the way she goes.


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OfflineJoeyFatone
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Re: Good growth in jar, or contams? [Re: Sockadin]
    #26465074 - 02/02/20 10:52 AM (3 years, 11 months ago)

I'm a fan of the first part of that sentence... Gives me a bit of hope... can't say I'm a fan of the second... all 6 jars are showing zero growth haha!


Edited by JoeyFatone (02/02/20 10:53 AM)


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OfflineJoeyFatone
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Re: Good growth in jar, or contams? [Re: JoeyFatone]
    #26465120 - 02/02/20 11:19 AM (3 years, 11 months ago)

Would the fact that I'm doing Golden Teachers change the colonization speed at all? I've read that some strains can take a bit longer to colonize.


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Re: Good growth in jar, or contams? [Re: JoeyFatone]
    #26465236 - 02/02/20 12:57 PM (3 years, 11 months ago)

Technically they are varieties of the same strain, some genetics are more prevelant in certain varieties if we are all talking about cubes. But the reason we isolate is to narrow down the dominate genes in that variety and get a tissue culture that does what we want. Fast growing, full pinset, big harvest, high potency, also looks.


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OfflineJoeyFatone
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Re: Good growth in jar, or contams? [Re: Sockadin]
    #26465677 - 02/02/20 05:28 PM (3 years, 11 months ago)

Quote:

Sockadin said:
Technically they are varieties of the same strain, some genetics are more prevelant in certain varieties if we are all talking about cubes. But the reason we isolate is to narrow down the dominate genes in that variety and get a tissue culture that does what we want. Fast growing, full pinset, big harvest, high potency, also looks.





Interesting. Thanks! So you don't think that I should be worrying about the fact that nothing started happening yet, even though t's ~day 7, son to be 8?


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Re: Good growth in jar, or contams? [Re: JoeyFatone]
    #26465948 - 02/02/20 08:44 PM (3 years, 11 months ago)

What’s your temperature like?

Also there’s a chance that it won’t germinate, happens a lot with syringes.


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OfflineJoeyFatone
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Re: Good growth in jar, or contams? [Re: jbgtaa]
    #26465965 - 02/02/20 08:56 PM (3 years, 11 months ago)

Quote:

jbgtaa said:
What’s your temperature like?

Also there’s a chance that it won’t germinate, happens a lot with syringes.




Well shit. I bought it from a reputable seller... One who's really well-reknowned and has a LOT of reviews, like all of them positive.

Is it a bad sign that it's this far in, and no growth as of yet? Should I be worried?

Temp is anywhere from 74-82, give or take.


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OfflineJoeyFatone
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Re: Good growth in jar, or contams? [Re: JoeyFatone]
    #26466027 - 02/02/20 09:45 PM (3 years, 11 months ago)

Oh. And I used fine verm for both the substrate as well as the protective verm layer. No tape, or anything like that. Is fine verm okay? It's all I could find. I didn't "pack it" in there.


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Re: Good growth in jar, or contams? [Re: JoeyFatone]
    #26466145 - 02/03/20 12:21 AM (3 years, 11 months ago)

Quote:

JoeyFatone said:
Quote:

jbgtaa said:
What’s your temperature like?

Also there’s a chance that it won’t germinate, happens a lot with syringes.




Well shit. I bought it from a reputable seller... One who's really well-reknowned and has a LOT of reviews, like all of them positive.

Is it a bad sign that it's this far in, and no growth as of yet? Should I be worried?

Temp is anywhere from 74-82, give or take.




Everyone said it can take up to 2 weeks before you see signs of growth. Stop asking if you should be worried for at least 7 more days. And if you see no growth after seven days, still don't ask if you should be worried, because you actually should be worried. Your jar looks fine, so don't worry, until then.


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Re: Good growth in jar, or contams? [Re: Raccoon]
    #26466347 - 02/03/20 06:50 AM (3 years, 11 months ago)

Raccoon is right, don’t stress. Your spores either will or won’t germinate. Doesn’t matter how reputable the vendor is, just because all of his ratings are positive doesn’t mean he can bend the course of nature to only sell viable spores. It’s far easier to germinate spores on agar than on the main substrate and give you wayyyy less chance of contamination. And you can see if your spores are viable before wasting a jar.


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OfflineJoeyFatone
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Re: Good growth in jar, or contams? [Re: jbgtaa]
    #26466371 - 02/03/20 07:11 AM (3 years, 11 months ago)

Thanks guys. Yeah I worry too much. One reason why I'm taking up this project.

So good news. Day 8? I think? If I counted right? I see one tiny cloth-y white spot showing up on one jar, and a smaller on on another. Hoping this is good! I think this is what I've been waiting for!

Also, when it comes to the fruiting chamber, I made one out of a plastic tote. I can't get all the "excess plastic bits" off, from where I drilled. Most I can, not all though. But they're not "clogged" as far as I can tell. Is this alright to go ahead and use once it's time, regardless of the plastic bits?

Thanks!


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Offlinecactushead
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Re: Good growth in jar, or contams? [Re: JoeyFatone]
    #26466411 - 02/03/20 08:01 AM (3 years, 11 months ago)

I made an account to reply to you. I think there is a possibility you are over thinking some of this. When you read the super helpful posts here, nobody ever says "think about every last millimeter of every tool and container you use, and never ever start until you are sure conditions are 100% perfect. Only then will you have 100% success." Its pretty much "chill out, use what you have, fix it if it doesn't work, and everybody's stuff fails now and then."

I don't have direct experience with how the shredded/chipped plastic stuff from drilling would affect a grow. I don't see how it really could? The colony is already going to be surrounded by plastic, I don't think it's going to run into some unevenly cut plastic and abort.

If it all goes to hell, the worst case scenario is you're out the cost of a syringe. You have all the time in the world to try again until you get it right.


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OfflineJoeyFatone
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Re: Good growth in jar, or contams? [Re: cactushead]
    #26466749 - 02/03/20 12:25 PM (3 years, 11 months ago)

Thanks, Cactus! And absolutely I am. I overthink everything. Again, which is one reason I want to do this project. To help me with my anxiety and overthinking...ness... haha!

But I did want to give some updates.

Nothing substantial, but at least there's a tiny bit of growth! Does this mean it'll "take off" soon?

The picture doesn't do it justice, but in clear-sight, it's a little white fuzzy dude. Optimistic!



Edited by JoeyFatone (02/03/20 12:26 PM)


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Re: Good growth in jar, or contams? [Re: JoeyFatone]
    #26466825 - 02/03/20 01:33 PM (3 years, 11 months ago)

Start your self help therepy now. Take a few days off from posting, relax, and just enjoy the process. Just observe it grow. It's gonna do what it's gonna do regardless. It looks good. We'll all be here when you get back from your break. Cheers!


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OfflineJoeyFatone
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Re: Good growth in jar, or contams? [Re: Raccoon]
    #26466832 - 02/03/20 01:39 PM (3 years, 11 months ago)

Quote:

Raccoon said:
Start your self help therepy now. Take a few days off from posting, relax, and just enjoy the process. Just observe it grow. It's gonna do what it's gonna do regardless. It looks good. We'll all be here when you get back from your break. Cheers!





Thanks man! Yeah, currently WIP. Meditating with a candle in my freetime because it's relaxing. Maybe playing my guitar. This isn't going to be a "fix" for me. Just hopefully to aid what I'm already working on. I'm not where I'd like to be yet, but I'm determined to take the steps necessary to become the best 'me'. And glad it's looking good! Thanks man! I appreciate you. Excited to see things progress with this. It sure is teaching me patience haha


Edited by JoeyFatone (02/03/20 01:48 PM)


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Re: Good growth in jar, or contams? [Re: JoeyFatone]
    #26466851 - 02/03/20 01:52 PM (3 years, 11 months ago)

Good luck on your journey. I can relate. Overanalyzing is one of my strengths, it really helps my research and my ability to learn. But it also prevents me from relaxing and achieving some level of ego death during meditation and trips. Lately my goal has been to just let go, no matter how important a thought or situation seems to be. As it pertains to your grow, be okay with some level of uncertainty. Let it go. Worse case is that it it fails to grow and you learn something. That's an amazing outcome, learning something. It sounds like you'll be fine though, with your attention to detail and good sterile practice.


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OfflineJoeyFatone
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Re: Good growth in jar, or contams? [Re: Raccoon]
    #26467804 - 02/04/20 01:09 AM (3 years, 11 months ago)

Thanks so much man. That really means a lot. Its one step at a time. Thank you! Glad to have such kindness here, thank you!

So i havent seen any growth at all since that last update. Does it not suddenly start growing rapidly once it appears? Its still just a dot of mycelium in the jar.

Maybe I should loosen the jar lids a little? They were on there rather tightly. Maybe since it's fine verm, it's not getting enough FAE.

GE, rather.*


Edited by JoeyFatone (02/04/20 09:41 AM)


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Re: Good growth in jar, or contams? [Re: JoeyFatone]
    #26468173 - 02/04/20 09:50 AM (3 years, 11 months ago)

I don't have experience with cakes, so I can't answer that. The growth will be a consistent rate of expansion. If it stalls, then you may not have enough GE, but that's up to you to decide. Sometimes people do to much and ruin their grow, so inaction is often the best choice. Maybe wait a day to see what happens first? But if your instinct says GE is restricted, then give a little more...


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OfflineJoeyFatone
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Re: Good growth in jar, or contams? [Re: Raccoon]
    #26468376 - 02/04/20 12:00 PM (3 years, 11 months ago)

Quote:

Raccoon said:
I don't have experience with cakes, so I can't answer that. The growth will be a consistent rate of expansion. If it stalls, then you may not have enough GE, but that's up to you to decide. Sometimes people do to much and ruin their grow, so inaction is often the best choice. Maybe wait a day to see what happens first? But if your instinct says GE is restricted, then give a little more...





I don't know... I'm getting really damn frustrated because I spent a fair amount of money getting this together, to help my anxiety, but nothing's really growing so it's only making me even more anxious. I'm getting stressed and pissed because I can't figure out what the hell the reason for it not growing is. I see one little "spot", on like 3 of the 6 jars that appeared a day or two ago, and since then, they haven't expanded, there's been no change... I have no idea. I just want it to fucking grow already.
I have absolutely no clue as to why the hell it's not growing, but I'm seriously getting annoyed at this point.

(Kidna venting. Rough day)


Edited by JoeyFatone (02/04/20 12:01 PM)


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Re: Good growth in jar, or contams? [Re: JoeyFatone]
    #26468735 - 02/04/20 03:20 PM (3 years, 11 months ago)

Don't look at them for another week. Not even a peek


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