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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: A.k.a]
    #26501280 - 02/24/20 11:03 AM (3 years, 10 months ago)

I’m going to post pictures later on tonight. When I checked this morning it had more growth. I just don’t know what to do next, where to take from, or if I still need to let it grow out before taking a piece. I will post picture after work.


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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26501330 - 02/24/20 11:26 AM (3 years, 10 months ago)

:thumbup:


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: c10h12n2o]
    #26501877 - 02/24/20 04:33 PM (3 years, 10 months ago)

So I have two agar plates that I inoculated what was left with my B+ syringe. The first one I did I tried pressing the plunger lightly, but it shot out a stream of several drops, that’s why it looks like that. The other one I managed to get one single drop that was hanging. These are no pours, so the visibility isn’t the absolute best, and I tried to take pictures on the sides without condensation. These are my two agar plates, what do you guys think and read from this? I’m not sure what to do now...? Let it grow out wider some more so I can get a good clean edge? I don’t see any distinct rhizo to take from. What do you guys think of these?



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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: A.k.a]
    #26501881 - 02/24/20 04:35 PM (3 years, 10 months ago)

Not sure what to do now. Let them grow wider to get some rhizo growth and then take a transfer? Looking at these plates what can you already read it? What is your experience telling you about these samples?



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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26501885 - 02/24/20 04:37 PM (3 years, 10 months ago)

Here you go gizmo, not sure what to do. This is just beginning growth, no rhizo edges yet, should I let it grow out more so I can take a piece of the edge later? Not sure what to do right now. Looking at the these, what are you opinions and what do you take from these? What do you read from these samples?



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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26502129 - 02/24/20 07:00 PM (3 years, 10 months ago)

Err very hard to see.
I'd try and get some clearer pics.
But In the 2nd pic the bottom colony(closest to the bottom of the pic)
I'd transfer the growth in that colony somewhere between 8-10 o'clock.
The plate with the single colony I'm thinking I see what looks like mold on that one I'd focus on the other for now.
Also I'd post in agar envy when you get better pics you will be more likely to get better help there.


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26502168 - 02/24/20 07:20 PM (3 years, 10 months ago)

Okay, I’ll try posting over there. Those are the best clear pics I can get through the glass. I don’t think I can get any better or more clear pics unless I let it grow more. Is there any danger in letting it grow more? What in that image indicates mold? And what led you to decision about the other one?


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Invisiblegizmo1
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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26502195 - 02/24/20 07:37 PM (3 years, 10 months ago)

Quote:

psycho_nauticus said:
Okay, I’ll try posting over there. Those are the best clear pics I can get through the glass. I don’t think I can get any better or more clear pics unless I let it grow more. Is there any danger in letting it grow more? What in that image indicates mold? And what led you to decision about the other one?



Well these are just guesses because I can only really see reflections on the pics can't see very well.... But the one with the single colony the outer growth looks weird kinda powdery looking. In my experience this indicates mold.
The other one the it's a guess because that's looks closest to cube mucellium the parts closest to the back could be healthy growth too but again I can't really. Tell. That's what sucks about no pours. The visibility is shit in most no pour containers.

Maybe try playing with the lighting it's really just the reflections that are making it hard to see the pics aren't horrible. Sometimes I will take 10 pics before I get one clear enough to post.


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Re: Agar for a noob like me? [Re: gizmo1]
    #26502250 - 02/24/20 08:16 PM (3 years, 10 months ago)

I'd take a few transfers from different places in the tomentose growth in the first plate.  I had B+ do this same thing and still got to where I wanted to be with a few more transfers.


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26502290 - 02/24/20 08:40 PM (3 years, 10 months ago)

These are the best pictures I could get playing with the light, or other wise I’m going have to start all over again from the beginning... The curvature from the glass is messing with it also...



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Edited by psycho_nauticus (02/24/20 08:46 PM)


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: InfiniteDreams]
    #26502292 - 02/24/20 08:41 PM (3 years, 10 months ago)

Where would that be? To my understanding isn’t the whole thing tomentose? So anywhere from it?


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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26502297 - 02/24/20 08:50 PM (3 years, 10 months ago)

Well, your newer pictures show that same concerning perimeter around the multi-colonies that are show up on the other plate.

What worked for me was transferring from the edge of the tomentose growth.  I took 8 total T1 across 2 plates.  Most of those plates failed.  I was able to get a set of T2 plates, 5 total.  Most of these plates failed.  But, 2 suddenly decided to take off with rhizomorphic growth so I am hopeful for the T3 plates I took from them.  Always transferring from the edge of the growth.  Use magnification if you going around the perimeter to look for helpful spots.

In contrast, my other variety is all rhizo starting on the T1s and I have to get rid of plates because they just aren't as good as others.  Transferred at the same time, in the same SAB, with the same agar formulas as the B+.


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Invisiblegizmo1
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Re: Agar for a noob like me? [Re: InfiniteDreams]
    #26502325 - 02/24/20 09:13 PM (3 years, 10 months ago)

Those pics are a little better.
I'd Post them all to the agar envy thread.
I'm thinking you will get more exposure and help there.

Are the first 3 pics all the same plate? It looks fine in the first 2 but the 3rd pic looks questionable.


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Re: Agar for a noob like me? [Re: gizmo1]
    #26502431 - 02/24/20 11:04 PM (3 years, 10 months ago)

You should do the serial streak method from my sig, with a drop or 2 of solution.

Makes it much easier to isolate clean myc from contams and get more distinct colonies with different characteristics


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26502604 - 02/25/20 05:32 AM (3 years, 10 months ago)

Yeah the single one is the same, I just tried to take multiple pictures. I actually did post over there a couple times, but I’m actually not getting any help. There’s just so many posts that it seems you just get constantly skipped. By the time I’m waiting for an answer, other people will of posted also, and the last one usually gets the help and answer. I seem to get answers and feedback here more quickly on my own, but I will try there again.

I’m confused if I should be taking transfers and from where. I was told from the tomentose mycelium, but aren’t they all tomentose? Or should I just cut one of the edges? I need help in learning what to transfer and what I’m looking at.


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: c10h12n2o]
    #26502610 - 02/25/20 05:38 AM (3 years, 10 months ago)

I’m confused, I thought streaking is for isolating colonies for certain genetics and stuff like that. I’m just trying to get some healthy mycelium to grain so I can grow.

So you’re supposed to streak to inoculate agar? I thought it’s just by dropping a drop of spore solution or bits of a print...


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Invisiblegizmo1
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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26502886 - 02/25/20 10:16 AM (3 years, 10 months ago)

Quote:

psycho_nauticus said:
I’m confused, I thought streaking is for isolating colonies for certain genetics and stuff like that. I’m just trying to get some healthy mycelium to grain so I can grow.

So you’re supposed to streak to inoculate agar? I thought it’s just by dropping a drop of spore solution or bits of a print...



It's not necessary but can be useful to streak.

Do you you see any growth that doesn't have that white powdery looking stuff surrounding it? Do the plates look much different at all today? Enough to post another pic?


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Invisiblec10h12n2o
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Re: Agar for a noob like me? [Re: gizmo1]
    #26503036 - 02/25/20 12:00 PM (3 years, 10 months ago)

Streaking spore solution or bits of a print (rather than dropping) is super useful for isolating healthy myc, cleaning contams, or for trying to isolate genetics. I'll link you a discussion about exactly your question

It's not always neccessary,  but I think its always beneficial, for the reasons explained here:

https://www.shroomery.org/forums/showflat.php/Number/26502229#26502229

When you just drop spores onto agar, countless spores and contams are all in a big pile, and grow through each other and intermingle to form a (usually very unorganized) colony

Streaking reduces the number of strains per colony,  and the the number of contams.

Basically,  it's like if you wanted to grow one rosebush in your yard, just dropping spores would be like digging a hole and pouring 100lbs of seeds in there. Serial streaking would be more like using seed starter trays to germinate many seeds individually,  then selecting the best looking sprout and planting it in your yard

Also keep in mind that spores are NEVER sterile, so streaking helps us get clean myc by giving us more to choose from on the plate and less contams in the later zones


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"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


Edited by c10h12n2o (02/25/20 12:02 PM)


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Invisiblec10h12n2o
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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26503068 - 02/25/20 12:16 PM (3 years, 10 months ago)

Quote:

psycho_nauticus said:
I’m confused if I should be taking transfers and from where. I was told from the tomentose mycelium, but aren’t they all tomentose? Or should I just cut one of the edges? I need help in learning what to transfer and what I’m looking at.




Honestly that ring around it does not look good, and the myc looks fairly powdery in the pic. Very unorganized in any case

But typically you would transfer the leading edge of the mycelium (in your case it might be bacterial though, because bacteria grows faster than myc)

Here is a pic with examples of suggested transfer areas:





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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26503418 - 02/25/20 03:31 PM (3 years, 10 months ago)

This is my agar today. I don’t see where I can transfer from. Everything is kind of all over and still powdery looking but thicker. Maybe I need to start with streaking, I don’t know. What do you think?



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