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Offlineyojay50
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Re: Agar for a noob like me? [Re: gizmo1]
    #26471375 - 02/06/20 02:04 AM (3 years, 11 months ago)

Quote:

gizmo1 said:
Quote:

psycho_nauticus said:
Thank you that sounds awesome! Thank you for your help and guidance also! I'm looking at my jars of millet hoping I've done everything correctly. I have germination, it looks to be germinating all down each side where I gave it a squirt and it ran down the glass. The growth doesn't have any hyphae mycelium tends to have, it's just started out fuzzy, but then again it's only day 4. I'm thinking all different crap like hopefully my millet isn't too dry, I should of shaken it before inoculating, maybe I should of used rye instead, I wish I would of learned agar first, all the classic signs of a noob lol. I need to post some pics. Hopefully it will really take off when I do the shake. I'm hoping this works out because the millet was a bitch to prep and so messy anyways that I know I'm not going to be using millet again. Hopefully it will really take off when I do the shake and redistribute any moisture and the mycelium gets really aggressive.



Im always happy to help when I can. Im not the best cultivator but ive been around for a long time so I know a little bit. I still learn something new myself all the time.  I know it can seem overwhelming at first but its not as hard as it sound. You will be doing agar like a pro in no time and you're not too proud to ask when you need help so thats always a good thing lol.




:rockon: what he said


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26471437 - 02/06/20 04:07 AM (3 years, 11 months ago)

Thanks. I’m still debating if I should get the premix or get the agar and LME separate. What would you do? Any specific brands of each I should look at that you use that you know are consistent?


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InvisibleInfiniteDreams
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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26471569 - 02/06/20 06:56 AM (3 years, 11 months ago)

I vote you mix your own because it gives you more control over your process.


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Invisiblegizmo1
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Re: Agar for a noob like me? [Re: InfiniteDreams]
    #26471865 - 02/06/20 11:34 AM (3 years, 11 months ago)



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Edited by gizmo1 (02/06/20 11:40 AM)


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26471899 - 02/06/20 11:53 AM (3 years, 11 months ago)

Those are actually the two I was looking at also. I’m ordering them right now. So do you use a media bottle and Petri dishes as well or just use a jar and some other containers instead will do?


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Invisiblegizmo1
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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26472033 - 02/06/20 01:05 PM (3 years, 11 months ago)

When doing no pours I use a measuring cup to mix and pour. For doing sterile petri dishes I use my media bottle. I do both still. For me startimg with no pours was the easiest way to get my feet wet.
Which method are you planning on using?


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26472155 - 02/06/20 02:15 PM (3 years, 11 months ago)

I wanted to just start with a no pour to at least inoculate with. How did you do your first no pour?


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Edited by psycho_nauticus (02/06/20 02:40 PM)


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26472274 - 02/06/20 03:33 PM (3 years, 11 months ago)

I just ordered everything I needed and should be coming over the weekend! I was thinking no pour, to at least make a plate to inoculate. How should I go about doing that? How did you do your no pour?


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Invisiblegizmo1
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Re: Agar for a noob like me? [Re: gizmo1]
    #26472408 - 02/06/20 05:01 PM (3 years, 11 months ago)

Quote:

gizmo1 said:
For no pours I like the little regular mourh half pint anchor brand jars that are sold at walmart about 7 bucks for a dozen. I use unmodified plastic lids with them. I leave the lids loose while pressure cooking then after they are cool I will tighten them down.
For agar I just mix 8 grams agar powder and 8 gram malt extract into 500ml water in a pyrex measuring cup and microwave until boiling. Stir until dissolved then pour maybe a 1/4" in the bottom of each jar.
I let them cool before adding the lids loosely. Then slap them im a pc vent pc for 10 minutes bring pressure up super slow. Pc for 25 minutes and bring pressure down super slow. Condensation free glass no pours. Simple as fuck and never have to worry about a warped container.



Just like this gimme a bit and I'll upload some pics to show a lil more.
Do you already have no pour containers figured out?


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26472578 - 02/06/20 06:58 PM (3 years, 11 months ago)

I was thinking maybe the little tiny mason jars they have, or some plastic Glad cup containers...


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Invisiblegizmo1
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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26472856 - 02/06/20 09:06 PM (3 years, 11 months ago)

These ones work pretty good.

They can be loaded with or without foil, modded or unmodified lids. I also like the plastic screwtop ziploc brand ones.


Edited by gizmo1 (02/06/20 09:12 PM)


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OfflineSYF8
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Re: Agar for a noob like me? [Re: gizmo1]
    #26472934 - 02/06/20 09:39 PM (3 years, 11 months ago)

Quote:

gizmo1 said:
These ones work pretty good.

They can be loaded with or without foil, modded or unmodified lids.




I’ve been thinking about using these with PastyWhyte’s tek.  Do they work well with the tiger drop method?  Any issues with moisture?


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Invisiblegizmo1
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Re: Agar for a noob like me? [Re: SYF8]
    #26472942 - 02/06/20 09:44 PM (3 years, 11 months ago)

Quote:

SYF8 said:
Quote:

gizmo1 said:
These ones work pretty good.

They can be loaded with or without foil, modded or unmodified lids.




I’ve been thinking about using these with PastyWhyte’s tek.  Do they work well with the tiger drop method?  Any issues with moisture?



No they dont tiger drop well. The agar puck is bigger than the mouth pf the jar.
They can have condensation issues but no worse than plastic no pours or petris. Its up to you to control condensation.  The main way i have found to get condensation free no pours is bringing temps up and down super slow. You can also try insulating on cool down.
Im not sureif its a problemin the summer but during winter condensation extra pisses me off.


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26473269 - 02/07/20 04:16 AM (3 years, 11 months ago)

Thanks, I need to look around and see what containers I can get. So GE holes aren’t necessary for agar correct?


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InvisibleLadysKnight
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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26473315 - 02/07/20 05:58 AM (3 years, 11 months ago)

I control condensation for no-pours by letting them fully cool without lids before the PC, then let them cool completely inside the PC before removing them. (If the agar is the same temp as ambient, there can be no condensation).


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26473333 - 02/07/20 06:35 AM (3 years, 11 months ago)

It sounds like doing agar isn’t going to be easy. If my jars aren’t even going correctly I feel like agar is just another thing that is also going to be difficult now that we’re talking about condensation and stuff. People were telling me agar is easy and will be doing it in no time, but seems pretty difficult with the worries about condensation now and other things. It sounds like you have to get everything totally perfect, one small thing also and agar is screwed up... And I just bought all the stuff for it... FML

It just adds to the overwhelming factor plus being frustrated.

It sounds like maybe doing another syringe to grain may be more advantageous than agar. I know I’m definitely not using millet again, I’m doing rye berry.


Edited by psycho_nauticus (02/07/20 06:37 AM)


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InvisibleLadysKnight
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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26473339 - 02/07/20 06:44 AM (3 years, 11 months ago)

Nope. You can not worry about the little stuff and everything will be fine. Do it for a while and you will start finding things that bother you and look for ways to improve it.

I've noticed that you like to panic. You don't have to. The proper way to do things has already been figured out and written down in several ways. All you have to do is pick a Tek, follow it exactly, and chill.


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OfflineA.k.aM
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Re: Agar for a noob like me? [Re: LadysKnight]
    #26473400 - 02/07/20 07:56 AM (3 years, 11 months ago)

Yeah you’ll be fine man condensation isn’t a big deal. Agar is so easy that little stuff like a foggy dish is the biggest thing to bitch about.

It’s the agar version of first world problems.


That said, I fucking hate condensation!


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Edited by A.k.a (02/07/20 07:57 AM)


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Invisiblegizmo1
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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26473488 - 02/07/20 08:57 AM (3 years, 11 months ago)

Quote:

psycho_nauticus said:
It sounds like doing agar isn’t going to be easy. If my jars aren’t even going correctly I feel like agar is just another thing that is also going to be difficult now that we’re talking about condensation and stuff. People were telling me agar is easy and will be doing it in no time, but seems pretty difficult with the worries about condensation now and other things. It sounds like you have to get everything totally perfect, one small thing also and agar is screwed up... And I just bought all the stuff for it... FML

It just adds to the overwhelming factor plus being frustrated.

It sounds like maybe doing another syringe to grain may be more advantageous than agar. I know I’m definitely not using millet again, I’m doing rye berry.



The reason we do agar is so the guess work is taken out and we know what we are growing in our jars. If tour jars didnt go good agar is exactly what you need. Its simple we arenjust growing out the spores on a flat surface then moving clean growth to grains. Oversimplified of course but thats all there is to it.
If you follow that little write up I gave you that was written with condensation in mind I hate condensation. Thats why it says leave the lids off until they cool then put the lids on loosely and the step about bringing pressure up and down super slow. Those are steps I learned over the years to help deal with condensation. Also like ladysknight said you want to let them cool in the pc.
You got this man dont let your thoughts get the better of you.


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26473578 - 02/07/20 09:45 AM (3 years, 11 months ago)

Cool thanks man. Whenever I do my first agar inoculation I’ll be on here asking you guys advice on what to do. I’m not sure what I need to look for on the agar, what is contaminant or not, and what and how to transfer. I’ll be bothering you guys later on again. Thank all you guys though!


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