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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26465565 - 02/02/20 04:13 PM (3 years, 11 months ago)

That’s actually the one I was looking at! There’s also a 56 gram one. I’m thinking of buying either one of these, but I don’t want to use the entire thing at once. I just wanted to start out with 5 or 10 plates, but the directions on there are for using the whole bag at once. I don’t know how to scale how much of the powder and water I would need just to start...


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InvisibleKimble
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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26465583 - 02/02/20 04:18 PM (3 years, 11 months ago)

Well make up a bottle, pour what you need and put the bottle in the fridge till you need the rest of it. No need to 'scale it'. That said, why not just pour all the plates in a sleeve in one go and store the unused ones. It seems like unnecessary work having to melt it and set up to pour again later when you could just do it once per sleeve.

Also what's the point of premix agar powder. You can't measure out 10g agar, 10g light malt extract and 500ml water? Yes you can, there is no reason to buy a premix other than lazyness. You're paying a premium for someone to weigh and mix XXXg of one powder with XXXg of another.

Nopour is good if you don't want to pour the hot liquid into sterile petris. Which people sometimes perceive as difficult. So these get prepared before putting into the PC for sterilization. Bare in mind you still have to open these plates to inoculate or do transfers like any agar plate. 


Pour is good because petri dishes are big and have nice visibility which helps spot contams. They also come pre sterile, no PC'ng the dishes before use. Of course you have to PC the agar you mixed up but that's not hard. If you're concerned about pouring the agar in the SAB go for Pastys no pour. If you feel confident and like the good visibility of using petris then go with Bod's easy AF agar tek.

Both types can be stored for a long time when unused.


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Edited by Kimble (02/02/20 04:45 PM)


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OfflineA.k.aM
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Re: Agar for a noob like me? [Re: Kimble]
    #26465621 - 02/02/20 04:54 PM (3 years, 11 months ago)

Yeah that’s what I meant by making your own is the same as pre mix.

You still have to weigh out powder and pour it in a bottle.

I just zero my scale with a little plastic cup on it and pour in 7gs of agar then pour lme til it hits 14g.


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Invisiblegizmo1
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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26466149 - 02/03/20 12:32 AM (3 years, 11 months ago)

Quote:

psycho_nauticus said:
That’s actually the one I was looking at! There’s also a 56 gram one. I’m thinking of buying either one of these, but I don’t want to use the entire thing at once. I just wanted to start out with 5 or 10 plates, but the directions on there are for using the whole bag at once. I don’t know how to scale how much of the powder and water I would need just to start...



So according to the prep instructions they give you should use 56g premix to 1 liter water so that would be 28g to 500 ml water or 14g to 250ml water.


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OfflineA.k.aM
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Re: Agar for a noob like me? [Re: gizmo1]
    #26466683 - 02/03/20 11:31 AM (3 years, 11 months ago)

I got that same one and ran 16g to 400ml, little on the soft side but it was fine.


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Invisiblegizmo1
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Re: Agar for a noob like me? [Re: A.k.a]
    #26466708 - 02/03/20 11:53 AM (3 years, 11 months ago)

Quote:

A.k.a said:
I got that same one and ran 16g to 400ml, little on the soft side but it was fine.



You'd think that would be closer to the right ratio. Assuming its somewhere close to being 50% agar and 50% malt extract. That would call for 20g to 500ml but who knows how much they put of what lol so I just calculated it off of the bags instructions for him.
I like to use 8 grams malt and 8 grams agar to 500ml water myself but I buy my agar from the local asian market and buy maltoferm in like 2lb bags off ebay. Shit lasts forever. Around 20 bucks will get me 75 grams agar and 2lbs maltoferm.


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26466805 - 02/03/20 01:04 PM (3 years, 11 months ago)

Thanks for helping me out and guiding me in the right direction! I only have 1/2 cc of B+ left, so I’m hoping that will be enough for me to try agar and then inoculate and grow a couple shoeboxes from the agar. I’m still trying to understand how all this agar stuff works, it just seems over whelming. I don’t know where to start from and there’s so much information on it, so thank you. I’m hoping this 1/2 cc of B+ will work for me, that’s all I got.


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Edited by psycho_nauticus (02/03/20 01:05 PM)


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OfflineA.k.aM
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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26466848 - 02/03/20 01:51 PM (3 years, 11 months ago)

You can clone from dried mushrooms too if you save some to put on agar.


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: A.k.a]
    #26466910 - 02/03/20 02:23 PM (3 years, 11 months ago)

So is what I am considering doing with the mix a pour agar?


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InvisibleInfiniteDreams
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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26466947 - 02/03/20 02:43 PM (3 years, 11 months ago)

Quote:

psycho_nauticus said:
So is what I am considering doing with the mix a pour agar?




I don't think you have said one way or the other.

Pour -> Get petri dishes.  They come sterile in plastic sleeves.  You sterilize the agar in another container (e.g. media bottle).  Then in a SAB you pour the sterile agar into the petri dishes one by one.

No pour -> You get small containers making sure that the material they are made from can withstand the PC (check the teks for examples).  You add the agar mix to the containers before you sterilize them.  That way when they come out of the PC they are ready to go after they have cooled down.


So with no pour the pouring happens before the sterilization.  You avoid the extra SAB step.  Petris are small and stackable though, so you can make a whole bunch in one go.  There are advantages/disadvantages to either approach, and it really comes down to personal preference.


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: InfiniteDreams]
    #26467069 - 02/03/20 04:04 PM (3 years, 11 months ago)

Oh okay, so do I take the exact same mixture and follow the exact same directions and just pour it into little cups and PC it and will turn out to be the same but it's already sterile? Or do I need to prep it differently if wanting to do no pour?


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InvisibleInfiniteDreams
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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26467509 - 02/03/20 08:42 PM (3 years, 11 months ago)

You use the same agar.

There are multiple teks available for both methods.  I suggest picking one and following it closely.


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OfflineSockadin
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Re: Agar for a noob like me? [Re: InfiniteDreams]
    #26467624 - 02/03/20 09:45 PM (3 years, 11 months ago)

If you have 1/2 CC of B+ you might want to do a pour agar into petri dishes so you can squirt 1 little drop and streak it across the dish then transfer from that. I found that the no pour PP5 containers are harder to work with and can be a bit of a PITA. If you can't see through the lid very well you can't know when to transfer your myc.


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Invisiblegizmo1
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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26467691 - 02/03/20 10:42 PM (3 years, 11 months ago)

Quote:

psycho_nauticus said:
Thanks for helping me out and guiding me in the right direction! I only have 1/2 cc of B+ left, so I’m hoping that will be enough for me to try agar and then inoculate and grow a couple shoeboxes from the agar. I’m still trying to understand how all this agar stuff works, it just seems over whelming. I don’t know where to start from and there’s so much information on it, so thank you. I’m hoping this 1/2 cc of B+ will work for me, that’s all I got.



How long until you have access to marketplace?


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26467988 - 02/04/20 07:04 AM (3 years, 11 months ago)

Not sure what that is or what you mean by that. It sounds helpful, what is it and how do I check?


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26468810 - 02/04/20 04:10 PM (3 years, 11 months ago)

I think you have to be on here 90 days before you can. I got like two more months.


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Invisiblegizmo1
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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26468877 - 02/04/20 05:04 PM (3 years, 11 months ago)

Hmm pretty far away. I think you have to also have a certain amount of posts. I was going to say I can send you a print but I only like to deal with people who have access to the marketplace. For security reasons.
Keep in contact with me and I can get a print to you that will last alot longer than a syringe. No need to buy more spores stick around and you will have more spores than you cannuse in no time lol.


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26469042 - 02/04/20 06:39 PM (3 years, 11 months ago)

Thank you that sounds awesome! Thank you for your help and guidance also! I'm looking at my jars of millet hoping I've done everything correctly. I have germination, it looks to be germinating all down each side where I gave it a squirt and it ran down the glass. The growth doesn't have any hyphae mycelium tends to have, it's just started out fuzzy, but then again it's only day 4. I'm thinking all different crap like hopefully my millet isn't too dry, I should of shaken it before inoculating, maybe I should of used rye instead, I wish I would of learned agar first, all the classic signs of a noob lol. I need to post some pics. Hopefully it will really take off when I do the shake. I'm hoping this works out because the millet was a bitch to prep and so messy anyways that I know I'm not going to be using millet again. Hopefully it will really take off when I do the shake and redistribute any moisture and the mycelium gets really aggressive.


Edited by psycho_nauticus (02/04/20 06:41 PM)


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Offlineyojay50
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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26469411 - 02/04/20 10:40 PM (3 years, 11 months ago)

pasty plates are a problem solver when it comes to starting agar. I STILL have problems with excess moisture on the pasty plates which can cause some weird shit sometimes. gonna try two holes with micropore to maybe deal with condesation.


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Re: Agar for a noob like me? [Re: psycho_nauticus] * 1
    #26471351 - 02/06/20 01:23 AM (3 years, 11 months ago)

Quote:

psycho_nauticus said:
Thank you that sounds awesome! Thank you for your help and guidance also! I'm looking at my jars of millet hoping I've done everything correctly. I have germination, it looks to be germinating all down each side where I gave it a squirt and it ran down the glass. The growth doesn't have any hyphae mycelium tends to have, it's just started out fuzzy, but then again it's only day 4. I'm thinking all different crap like hopefully my millet isn't too dry, I should of shaken it before inoculating, maybe I should of used rye instead, I wish I would of learned agar first, all the classic signs of a noob lol. I need to post some pics. Hopefully it will really take off when I do the shake. I'm hoping this works out because the millet was a bitch to prep and so messy anyways that I know I'm not going to be using millet again. Hopefully it will really take off when I do the shake and redistribute any moisture and the mycelium gets really aggressive.



Im always happy to help when I can. Im not the best cultivator but ive been around for a long time so I know a little bit. I still learn something new myself all the time.  I know it can seem overwhelming at first but its not as hard as it sound. You will be doing agar like a pro in no time and you're not too proud to ask when you need help so thats always a good thing lol.


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