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InfiniteDreams


Registered: 10/25/19
Posts: 1,224
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Re: Agar for a noob like me? [Re: gizmo1]
#26516382 - 03/04/20 12:09 PM (3 years, 10 months ago) |
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My experience to, dry spores to agar, visible germ in 3-5 days. These were not ancient prints or anything though.
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c10h12n2o
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I've had at least 10 prints NEVER germinate. Tried all of them on various ph, soft agar, hard agar, forced hydration, various ph, charcoal... NOTHING seems to work
OP used 7g agar and 10g LME in 500ml. Ofc that will work, but you can expect denser , slower growth with that much LME, exactly like his pics
30g LME, 20g agar, 1L water is technically standard MEA, but for shroom purposes most people use half strength (15g LME) or weaker, because it makes it faster and more spread out (easier to work with). People oftwn use higher amounts for spore germination though
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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psycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
#26516679 - 03/04/20 03:03 PM (3 years, 10 months ago) |
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Yeah Gizmo, there's so much to learn every single step of the way, and every single day. I'm learning a lot more than I ever thought I would have going through trial and error myself. I appreciate all your input and help also. I made the rest of my no pour my 7 grams agar/ 10 LME/ 500 mL for swiping and streaking since I got a print, and for transfers also. I wanted something that would work for everything. I didn't think it was that big of a deal, oh well It was trusted advice from someone I befriended in this community just like I trust the advice you give me!
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psycho_nauticus
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Re: Agar for a noob like me? [Re: PNat]
#26516704 - 03/04/20 03:21 PM (3 years, 10 months ago) |
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Yeah I want this thread to keep on giving like it has been helping me. This is for all agar noobs!!!!
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PNat
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I got a lot of knowledge out of it as well, there were lots of questions that you ask and I had the same questions, too. So definitely, I totally agree homie!
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psycho_nauticus
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Re: Agar for a noob like me? [Re: c10h12n2o]
#26517224 - 03/04/20 07:26 PM (3 years, 10 months ago) |
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Does anyone have a clue what these little spots are? I wanted to take a some transfers from this growth and saw this. They look like three tiny tiny droplets, but this glass is condensation free with none at the top either, so nothing could of fell... They’re placed almost exactly in a triangle around the growth. It’s at the 1,4, and 9 o’clock positions. Answers or ideas?
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gizmo1



Registered: 06/15/11
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Quote:
psycho_nauticus said: Does anyone have a clue what these little spots are? I wanted to take a some transfers from this growth and saw this. They look like three tiny tiny droplets, but this glass is condensation free with none at the top either, so nothing could of fell... They’re placed almost exactly in a triangle around the growth. It’s at the 1,4, and 9 o’clock positions. Answers or ideas?

Not sure maybe some metabolites? Either way the mycellium is growing funny around them like its trying to avoid it so I'd do the same. Avoid those spots and transfer. Is that a grain on agar? Or is it poofy in the middle.
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A.k.a
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Re: Agar for a noob like me? [Re: gizmo1]
#26517495 - 03/04/20 09:34 PM (3 years, 10 months ago) |
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Looks like water to me but who knows. I’ve never seen a contam form a droplet like that before, bacteria is always slimy and a lot of times a perfect circle.
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LAGM2020     
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gizmo1



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Re: Agar for a noob like me? [Re: A.k.a]
#26517527 - 03/04/20 09:46 PM (3 years, 10 months ago) |
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It looks like water to me too but the mycelium is avoiding it so something weird may be going on. Also bacteria is hardly ever i a perfect circle from what I've seen. Sometimes it can be but alot of times the stuff that is hard to spot kinda goes everywhere. I mean I guess it also depends on how the bacteria was introduced because most of the time bacteria sits there and doesn't spread much. I got a plate with some crazy looking bacteria though hold up I'll grab a pic.
 This almost looks like mycellium but its bacteria. From a plate i poured out of a sleeve that sat around for about 5 years with a hole in it even found a dead fruit flynin the sleeve but poured them to see what happened. Didn't see the bacteria until after I threw a wedge on it lol.
Edited by gizmo1 (03/04/20 09:58 PM)
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A.k.a
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Re: Agar for a noob like me? [Re: gizmo1]
#26518738 - 03/05/20 01:45 PM (3 years, 10 months ago) |
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Yeah the myc avoiding it is odd.
When I get noticeable bacteria it’s usually either a big wet mess or colored circles like on this plate

Wet mess
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LAGM2020     
Edited by A.k.a (03/05/20 01:47 PM)
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gizmo1



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Re: Agar for a noob like me? [Re: A.k.a]
#26519055 - 03/05/20 04:29 PM (3 years, 10 months ago) |
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Quote:
A.k.a said: Yeah the myc avoiding it is odd.
When I get noticeable bacteria it’s usually either a big wet mess or colored circles like on this plate

Wet mess

Lmao yea the stuff I've seen is usually the wet mess stuff. Slimey yellow nastiness.
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yojay50
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Re: Agar for a noob like me? [Re: gizmo1]
#26520470 - 03/06/20 11:36 AM (3 years, 10 months ago) |
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my my recent set of plates was done by eye. my girlfriend made potatoes so i used the water from that and water from my wheat prep. i mixed the two and made used potato water and grain water no dextrose (sugar) and they are growing well. growing at 64f average temp. its tragically slow but it goes. got an aquarium heater so i found my solution to temps. the temp really blows.
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c10h12n2o
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Re: Agar for a noob like me? [Re: yojay50]
#26520539 - 03/06/20 12:21 PM (3 years, 10 months ago) |
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Why not just use a space heater to keep the room in the 70s?
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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psycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
#26537268 - 03/15/20 07:23 PM (3 years, 10 months ago) |
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Does it really matter if your agar transfers lay up or down when you got them on the agar? It will eventually colonize the agar same right? There's no harm in it is there? Or it always has to be facedown?
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gizmo1



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Mine land face up almost 100% of the time. I'd say it's not worth the extra risk of contamination trying to get the mycellium side down. Just leave it however it first touches down on the receiving plate.
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A.k.a
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Doesn’t matter. As long as it gets there you’re good. I usually try and get it myc side down but it really doesn’t seem to make much difference.
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LAGM2020     
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psycho_nauticus
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Re: Agar for a noob like me? [Re: A.k.a]
#26541167 - 03/17/20 04:48 PM (3 years, 10 months ago) |
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Whenever you take transfers from your colonizing streak plate, are they counted as T1? Or is that still T0? I swabbed the AA+, took a couple transfers from that streak plate, and then did another set of transfers from the ones I already took. I'm learning and trying to find out what transfer I'm on? What counts and does not count as a transfer? Am I on T1 or T2? I'm not sure if I'm supposed to count what I took from the streak plate when I swabbed it as T1 or not.
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A.k.a
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The spore plate is t0, so anything taken from that plate is T1. Once the wedge taken from the spore plate grows out anything taken from that plate will be t2.
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LAGM2020     
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psycho_nauticus
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Re: Agar for a noob like me? [Re: A.k.a]
#26541412 - 03/17/20 07:28 PM (3 years, 10 months ago) |
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Got it. Yeah I'm going to use these T2s once they grow out a little more. Whenever inoculating is it okay to include the transfer wedge in the middle? Or does it just need to be the mycelial growth around it without the wedge?
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gizmo1



Registered: 06/15/11
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Quote:
psycho_nauticus said: Got it. Yeah I'm going to use these T2s once they grow out a little more. Whenever inoculating is it okay to include the transfer wedge in the middle? Or does it just need to be the mycelial growth around it without the wedge?
If you are sure it's clean it doesn't matter I just started using the entire plates cut like a pie but I also do like 7 transfers first until I get very uniform growth. I'd avoid it for now. Idk of that's just something I do or if it's common practice but just avoid it for now until you are sure you've got good clean cultures.
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