|
psycho_nauticus
Stranger



Registered: 01/26/20
Posts: 779
Loc: Incognito on Cloud 9
Last seen: 7 months, 5 days
|
Re: Agar for a noob like me? [Re: c10h12n2o]
#26513403 - 03/02/20 06:36 PM (3 years, 10 months ago) |
|
|
Yeah I meant that even though that wasn’t my original piece of transfer wedge, taking a transfer from there would still count as T2 even though it’s not coming off my actual transfer wedge.
--------------------
|
c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
|
|
No, that doesnt make sense.
If you are transferring from the plate you germinated spores on, the new plate will be t1. No matter how many transfers you take from it, anything from that plate will be t1.
T2 plates will come from myc you transfer from t1 plates. T1 plates come from myc transferred from the spore germination/clone plate
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
|
psycho_nauticus
Stranger



Registered: 01/26/20
Posts: 779
Loc: Incognito on Cloud 9
Last seen: 7 months, 5 days
|
Re: Agar for a noob like me? [Re: c10h12n2o]
#26513435 - 03/02/20 06:55 PM (3 years, 10 months ago) |
|
|
The two closer to the edge are T1. Everything on that plate is T1. So I could take the edge of those right and then I would have a T2? Usually isn’t the growth and everything better once you get to transfer 3?
--------------------
|
c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
|
|
Yeah transfers from your t1 plate will be t1.
I would wait a few days and give growth chance to differentiate and form sectors
It's not inherently better after 3 transfers. However if you transfer from the best sectors , it usually is. What you have is too soon to form sectors distinct enough to distinguish/transfer, so you need to wait a bit
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
|
PNat
Stranger

Registered: 01/15/20
Posts: 50
Loc: Texas
Last seen: 1 year, 7 months
|
|
Quote:
C12ShroomMan said: I'm just starting too so figured i'd try both. These seem to be the best 'non -pour' containers, good vision,size, no taping needed, reusable https://www.shroomery.org/forums/showflat.php/Number/26374264
While i wait for those i bought a 20pk sterile petri dishes, Pouring them in a SAB wasn't too bad.
Where can I order those best non-pour containers? I cannot find them anywhere.
|
psycho_nauticus
Stranger



Registered: 01/26/20
Posts: 779
Loc: Incognito on Cloud 9
Last seen: 7 months, 5 days
|
Re: Agar for a noob like me? [Re: c10h12n2o]
#26514157 - 03/03/20 08:19 AM (3 years, 10 months ago) |
|
|
So it’s weird because my original transfer piece isn’t really spreading out any mycelium, while the other two colonies where I originally dropped it is growing out mycelium rapidly. Now I know you said that is because it is a smaller volume and and will be quicker, but you don’t think that’s good to take a transfer from. I don’t understand why my original piece isn’t really spreading out mycelium. My wedge in fact just seems to be getting a thicker growth of mycelium all over, like how a drop of spore solution does, but there is nothing actually spreading and colonizing from it. That’s been one week since I did that transfer. Yet the other two colonies are have more more growth around.
--------------------
|
PNat
Stranger

Registered: 01/15/20
Posts: 50
Loc: Texas
Last seen: 1 year, 7 months
|
|
After read all the pages on this thread, I think you got a couple of weeks of agar work before me. I am going through the same thing. I am working with Cambodian and Hawaiians. I am having trouble with Cams being fluffy and Hawaiian are doing very well with lots of rhizo. Just be patient but if you see bacteria, thats when you should panic and transfer. Other than that just let it get to like a dime or quarter size and then decide. I'll show you mine.
|
psycho_nauticus
Stranger



Registered: 01/26/20
Posts: 779
Loc: Incognito on Cloud 9
Last seen: 7 months, 5 days
|
Re: Agar for a noob like me? [Re: PNat]
#26514271 - 03/03/20 09:31 AM (3 years, 10 months ago) |
|
|
Dude that looks way better than mine, and you started after me??? My first transfers I did aren’t even really growing anything or spreading, you’re already on T3!!! I feel like I’m doing something wrong or not doing something correctly...
--------------------
|
PNat
Stranger

Registered: 01/15/20
Posts: 50
Loc: Texas
Last seen: 1 year, 7 months
|
|
Lots or trials and errors for the last 3 weeks, like what you are going through. Started with some pre poured petri dish. That didn't do very well. So I decided to follow a tek and I found c10's tek to be my liking. Invest in a media bottle and some petri dish and poured my own. Streaking with a loop didn't work for me at all. So I stuck with just dropping a single drop in the middle of the plate. Seems like all my plates take about 3 days to show something. But like after the 5th day, thats when I start thinking to make a move. Condensation was a PROBLEM, but now I just don't worry about it. I am still a noob but to me, I think you should invest in petri dish.
|
c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
|
Re: Agar for a noob like me? [Re: PNat]
#26514703 - 03/03/20 02:19 PM (3 years, 10 months ago) |
|
|
Quote:
psycho_nauticus said: Dude that looks way better than mine, and you started after me??? My first transfers I did aren’t even really growing anything or spreading, you’re already on T3!!! I feel like I’m doing something wrong or not doing something correctly...
He probably used a correct agar formula lol
Quote:
PNat said: Lots or trials and errors for the last 3 weeks, like what you are going through. Started with some pre poured petri dish. That didn't do very well. So I decided to follow a tek and I found c10's tek to be my liking. Invest in a media bottle and some petri dish and poured my own. Streaking with a loop didn't work for me at all. So I stuck with just dropping a single drop in the middle of the plate. Seems like all my plates take about 3 days to show something. But like after the 5th day, thats when I start thinking to make a move. Condensation was a PROBLEM, but now I just don't worry about it. I am still a noob but to me, I think you should invest in petri dish.
Glad you found it helpful!
Maybe your loop was too hot when you scraped the spores?
Looks like you have something good on that Hawaii plate! Transfer it asap before that bacterial splotch spreads into the myc
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
|
psycho_nauticus
Stranger



Registered: 01/26/20
Posts: 779
Loc: Incognito on Cloud 9
Last seen: 7 months, 5 days
|
Re: Agar for a noob like me? [Re: c10h12n2o]
#26514948 - 03/03/20 04:35 PM (3 years, 10 months ago) |
|
|
I know you’re still bashing me about my agar, but it was recommended to me by an experienced cultivator who knows what he is doing. I trust him even though everyone is telling me I fucked up!!!
--------------------
|
c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
|
|
Quote:
psycho_nauticus said: I know you’re still bashing me about my agar, but it was recommended to me by an experienced cultivator who knows what he is doing. I trust him even though everyone is telling me I fucked up!!!
Lol no one is bashing you, no one cares enough to be emotionally invested in what you do or dont do. You asked why other peoples look better, we answered.. Calm down, dial back the defensiveness, and read more carefully
anyone responding to your thread is taking the time to try to help you and others in your position. Whether or not you can be helped is entirely up to you
AGAIN you got the recipe backwards, and your plates look exactly like any experienced grower would expect them to look using that much LME (slow, thick growth)
Like the guy told you in the other thread, you got whatever someone recommended to you backwards
Quote:
footpath said: 7g LME / 10g agar / 500mL H2O is the standard recipe. So yeah, yours is backwards.
Following instructions correctly makes a big difference in this hobby
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
Edited by c10h12n2o (03/03/20 06:01 PM)
|
PNat
Stranger

Registered: 01/15/20
Posts: 50
Loc: Texas
Last seen: 1 year, 7 months
|
Re: Agar for a noob like me? [Re: c10h12n2o]
#26515238 - 03/03/20 06:47 PM (3 years, 10 months ago) |
|
|
Quote:
c10h12n2o said:
Quote:
psycho_nauticus said: Dude that looks way better than mine, and you started after me??? My first transfers I did aren’t even really growing anything or spreading, you’re already on T3!!! I feel like I’m doing something wrong or not doing something correctly...
Quote:
He probably used a correct agar formula lol
The formula that I use was 10 agar 7.5 LME and 500 H2O.
Quote:
PNat said: Lots or trials and errors for the last 3 weeks, like what you are going through. Started with some pre poured petri dish. That didn't do very well. So I decided to follow a tek and I found c10's tek to be my liking. Invest in a media bottle and some petri dish and poured my own. Streaking with a loop didn't work for me at all. So I stuck with just dropping a single drop in the middle of the plate. Seems like all my plates take about 3 days to show something. But like after the 5th day, thats when I start thinking to make a move. Condensation was a PROBLEM, but now I just don't worry about it. I am still a noob but to me, I think you should invest in petri dish.
Glad you found it helpful!
Maybe your loop was too hot when you scraped the spores?
Looks like you have something good on that Hawaii plate! Transfer it asap before that bacterial splotch spreads into the myc
I couldn't scrape the spores because I only had SS. So I tried to push a drop onto my loop which was very difficult. The plate later only had yellow dots to where I had streak them.
I did do a 4th transfer on the Hawaii already. And THANKS again c10, I tried to give you a rating but my post is low.
|
c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
|
Re: Agar for a noob like me? [Re: PNat]
#26515254 - 03/03/20 06:56 PM (3 years, 10 months ago) |
|
|
Quote:
PNat said:
The formula that I use was 10 agar 7.5 LME and 500 H2O.
Exactly lol.. that's perfect, exactly what I use and most other people use. OP got the numbers backwards and used way too much LME
Using too much or too little doesnt mean it won't work, it's just not ideal (depending on what you are trying to do)
Quote:
PNat said: I couldn't scrape the spores because I only had SS. So I tried to push a drop onto my loop which was very difficult. The plate later only had yellow dots to where I had streak them.
I did do a 4th transfer on the Hawaii already. And THANKS again c10, I tried to give you a rating but my post is low.
Yeah it can be tricky with SS because spores love to clump together
I appreciate it 
Glad you found it helpful. Imma go ahead and rate you so you aren't shroom less anymore ;]
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
|
PNat
Stranger

Registered: 01/15/20
Posts: 50
Loc: Texas
Last seen: 1 year, 7 months
|
Re: Agar for a noob like me? [Re: c10h12n2o]
#26515359 - 03/03/20 07:49 PM (3 years, 10 months ago) |
|
|
THANK YOU for your kindness and kind words, it really lift a noob like me confidence and spirit up!
|
PNat
Stranger

Registered: 01/15/20
Posts: 50
Loc: Texas
Last seen: 1 year, 7 months
|
Re: Agar for a noob like me? [Re: PNat]
#26515501 - 03/03/20 09:10 PM (3 years, 10 months ago) |
|
|
OP, I find this thread very useful for us beginners. I hope you can accomplish what you want. You can hit me up anytime if you want to discuss noob stuff, lol. We all learning.
|
gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
|
Re: Agar for a noob like me? [Re: PNat]
#26515694 - 03/04/20 12:38 AM (3 years, 10 months ago) |
|
|
I'll be honest im a little behind on this thread so what agar recipe are you usimg OP? 10grams lme 10 grams malt extract to 500ml water? Thats not bad at all used to be pretty standard. Now 10 is considered high for malt extract as well as agar. I like to use 8 and 8. 7 malt and 10 agar is cool but since when is that the standard? Not knocking it just the first im ever hearing of this being the standard.
|
A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 1 hour, 35 minutes
|
Re: Agar for a noob like me? [Re: gizmo1]
#26516259 - 03/04/20 10:31 AM (3 years, 10 months ago) |
|
|
The high nute lower agar mix is partially because some of the plates will be used for spores.
The great thing about agar is anything will work. I’ve used some pretty extreme recipes just to try them and they were all good.
The only total failures I’ve had were spores that wouldn’t germinate on tougher plates.
--------------------
LAGM2020     
|
InfiniteDreams


Registered: 10/25/19
Posts: 1,224
|
Re: Agar for a noob like me? [Re: c10h12n2o]
#26516305 - 03/04/20 11:16 AM (3 years, 10 months ago) |
|
|
Quote:
c10h12n2o said: Following instructions correctly makes a big difference in this hobby
I'd argue this is true for most things in life.
|
gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
|
Re: Agar for a noob like me? [Re: A.k.a]
#26516338 - 03/04/20 11:34 AM (3 years, 10 months ago) |
|
|
Ive never had a problem with spores straight from s print germinatimg on stiff agar. It may take a little longer but its always successful. Only reason I dialed my recipe back from 10 grams agar is for resource conservation. I noticed there honestly wasnt much difference between usimg 8 9 and 10g agar to 500ml. I figured if i can get away with less why not. Ive played with agar alot too and the only time I noticed a difference is when going with super high nutes. It woild cause the mycellium to crawl super slow. Noticed some slow growth with super low nutes too. Not as slow but I think if you go too far in either direction its bad. Other than that there is a broad spectrum of what works. Of course I should mention a new grower should follow a recipe. Especially on their first couple trys but the way OP is going about this is not bad at all learning through trial and error atleast in my experience is the best way to learn. I learn way better by fucking something up myself than by hearing about others fuck ups. Maybe it's just me but I won't make the same big mistake twice lmao. Live and learn. Plus we are here to guide in the right direction.
|
|