|
psycho_nauticus
Stranger



Registered: 01/26/20
Posts: 779
Loc: Incognito on Cloud 9
Last seen: 7 months, 5 days
|
Re: Agar for a noob like me? [Re: c10h12n2o]
#26503428 - 02/25/20 03:35 PM (3 years, 10 months ago) |
|
|
Nothing of mine looks like that. I don’t want to transfer anything that still looks bacterial. I don’t even know where I would transfer from in mine.
So you’re basically saying to streak and then only transfer from the last quadrant? My agar now anyways is past the point of transferring isn’t it? Nothing is uniform, it’s all over the place and powdery.
--------------------
|
yojay50
always learning


Registered: 04/21/17
Posts: 342
Last seen: 2 months, 1 day
|
|

Just transfer from anywhere
|
psycho_nauticus
Stranger



Registered: 01/26/20
Posts: 779
Loc: Incognito on Cloud 9
Last seen: 7 months, 5 days
|
|
I wanted to do a transfer at least today. Where would you transfer from? I put up new pics today. At this stage does it matter? Is whatever I transfer going to be inherently better and cleaner than what I have already?
--------------------
|
yojay50
always learning


Registered: 04/21/17
Posts: 342
Last seen: 2 months, 1 day
|
|
From what I've seen once you transfer you are isolating some genes.
It will look better I feel
|
gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
|
|
Take some transfers from several spots. Then do a hot pour. I don't see any bacteria there but with the way the mycellium is spreading it very well may be present. This is where these no pours shine. To do a hot pour just crack the lid and heat up the agar in a microwave. Then transfer to your SAB. Wait for the air to settle and pour the hot agar onto the original plate.
|
c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
|
Re: Agar for a noob like me? [Re: gizmo1]
#26504091 - 02/25/20 09:16 PM (3 years, 10 months ago) |
|
|
Powdery usually means its mold. Again, typically you transfer from the leading edge of the myc
I would streak then transfer colonies asap, like I explained
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
|
gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
|
Re: Agar for a noob like me? [Re: c10h12n2o]
#26504137 - 02/25/20 09:55 PM (3 years, 10 months ago) |
|
|
Quote:
c10h12n2o said: Powdery usually means its mold. Again, typically you transfer from the leading edge of the myc
I would streak then transfer colonies asap, like I explained
Bud. I know you're really not up to date on this thread but he is completely out of spore solution.
That's why he's trying so hard to make these plates work.
Offer is still up for some free prints if you need tho op. You will have a harvest soon if we have anything to do with it. I got your back. At least 10 pages worth of got your back lmao.
I really want you to succeed if not I wouldn't have been here this long trying to help. You remind me alot of myself when I started about 10 years ago. Especially when you panic lol. I had no chill, that only came with age and experience.
|
c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
|
Re: Agar for a noob like me? [Re: yojay50]
#26504195 - 02/25/20 11:04 PM (3 years, 10 months ago) |
|
|
Quote:
gizmo1 said: Bud. I know you're really not up to date on this thread but he is completely out of spore solution.
That's why he's trying so hard to make these plates work.
Yeah I didnt catch that part, i was just responding to his question on the last page asking if he should start with streaking
Looks like he used a ton of spore solution per plate , way too much
Quote:
psycho_nauticus said: I wanted to do a transfer at least today. Where would you transfer from? I put up new pics today. At this stage does it matter? Is whatever I transfer going to be inherently better and cleaner than what I have already?
You should definitely take gizmo up on his offer
Not INHERENTLY better, but it will make things easier to see and if contams are present it will (maybe) give you a chance to grab clean myc on the 2nd transfer
Transfer the leading edge of the myc, the point farthest from the origin
Quote:
yojay50 said: From what I've seen once you transfer you are isolating some genes.
It will look better I feel
Not Genes. Genes are genetic code of a particular individual (strain). You transfer part of a colony , which then grows out in 360 degrees, often forming various sectors which are made of different groups of strains.
In multispore, these sectors will still be made of multiple strains (each with a distinct combination of genes) growing through/over each other. Transfereing and selecting sectors allows you to select for characteristics like rhizo growth, fast growth, and clean growth, and it reduces the # of intermingled types of mycelium , which makes growth more organized. This is why cultures "look better" after a few transfers, like you said
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
|
psycho_nauticus
Stranger



Registered: 01/26/20
Posts: 779
Loc: Incognito on Cloud 9
Last seen: 7 months, 5 days
|
Re: Agar for a noob like me? [Re: c10h12n2o]
#26504473 - 02/26/20 07:56 AM (3 years, 10 months ago) |
|
|
I’m going to do my first transfers tonight. Is it true that you shouldn’t take transfers after too long because additional things grow along with it? Mine has been a week and half, so do you think I’m still good to take a transfer tonight?
--------------------
|
psycho_nauticus
Stranger



Registered: 01/26/20
Posts: 779
Loc: Incognito on Cloud 9
Last seen: 7 months, 5 days
|
Re: Agar for a noob like me? [Re: gizmo1]
#26504480 - 02/26/20 08:07 AM (3 years, 10 months ago) |
|
|
It’s been about a week and half, and I’m going to take transfers tonight. Is a week and half too long to take transfers? I’ve read if you wait too long other stuff grows with the mycelium also. Do you think I’m good to still take transfers a week and half in?
--------------------
|
c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
|
|
You can still transfer it. I was talking about in a streak plate, it doesnt matter so much the way you did it because everything is growing over/through each other anyway, because it started from a giant pile of spores
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
|
gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
|
|
Quote:
psycho_nauticus said: It’s been about a week and half, and I’m going to take transfers tonight. Is a week and half too long to take transfers? I’ve read if you wait too long other stuff grows with the mycelium also. Do you think I’m good to still take transfers a week and half in?
It is good practice to transfer sooner but its not a game stopper if you transfer a little late. You usually want to transfer when you have growth about the size of a US quarter for transfer plates and just whenever you see clean growth on a germ plate. If you grab a transfer a little earlier or later no big deal, but you do want to avoid letting the growth reach to the edges. Supposedly this can cause the mycelium to lose some of its vigor, but also the edges are more likely to have contamination thats fallen in than the center and if the plate is overgrown you cant grab the leading edge. Atleast thats how it works in my mind lol. If one of my plates ever does reach the edges I just avoid the edges when transferring, ideally you will want to catch it before it ever gets this far though. I only let my plates grow all the way out if I know it came from a clean culture and im planning on using the entire plate as inoculate. Hope this helps some.
|
psycho_nauticus
Stranger



Registered: 01/26/20
Posts: 779
Loc: Incognito on Cloud 9
Last seen: 7 months, 5 days
|
Re: Agar for a noob like me? [Re: gizmo1]
#26505069 - 02/26/20 03:44 PM (3 years, 10 months ago) |
|
|
Well I did my first transfers. I’m not going to lie, it was hard and awkward in the SAB. I feel like I fucked up and probably contaminated. It was hard cutting a small piece of agar from the edge, and then it wouldn’t come off my knife when transferring it, etc. It did not go smoothly... It was like one big cluster fuck
--------------------
|
A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 1 hour, 52 minutes
|
|
Hahaha yeah man don’t worry that’s everybodys first SAB session. Even if you contaminated the plates you’ll be good, that’s what plates are for.
--------------------
LAGM2020     
|
psycho_nauticus
Stranger



Registered: 01/26/20
Posts: 779
Loc: Incognito on Cloud 9
Last seen: 7 months, 5 days
|
Re: Agar for a noob like me? [Re: A.k.a]
#26505134 - 02/26/20 04:22 PM (3 years, 10 months ago) |
|
|
I'll have to make more. I only had four plates left, and took two transfers from each. Using a #11 blade is hard, cutting the agar, and then trying to get it to come off the sharp blade to transfer it. Lots of bumbling and fumbling around. What will they look like if they got contaminated? What should I look out for?
|
psycho_nauticus
Stranger



Registered: 01/26/20
Posts: 779
Loc: Incognito on Cloud 9
Last seen: 7 months, 5 days
|
Re: Agar for a noob like me? [Re: gizmo1]
#26509314 - 02/29/20 07:16 AM (3 years, 10 months ago) |
|
|
How does everyone get such fast growth on their agar and are able to transfer in just a couple days? I'm still waiting on my first transfer to show growth, and that was from a couple days ago already. After I let it grow out some then I have to do at least a second and third transfer, it seems it will take weeks to a month. How are people doing transfers in just a couple days???
--------------------
|
Sunny Skies
Cluster Head


Registered: 05/03/17
Posts: 421
Loc: my house
|
|
I made up some 2 weeks ago and made a transfer..growth seems to have stalled,I used powdered agar and dry malt..first time using powders..thinking about just using the agar powder with potato flakes and Karo and see how that works.
|
A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 1 hour, 52 minutes
|
Re: Agar for a noob like me? [Re: Sunny Skies]
#26509548 - 02/29/20 10:19 AM (3 years, 10 months ago) |
|
|
It usually takes like 3 days minimum to get enough to transfer. I have had some quick cultures that seemed to have zero recovery time.
But yeah it gets time consuming that’s why I was saying just transfer ASAP to get to t3 then let it grow out some.
Most of my plates take 2-3 weeks to fill up.
--------------------
LAGM2020     
|
psycho_nauticus
Stranger



Registered: 01/26/20
Posts: 779
Loc: Incognito on Cloud 9
Last seen: 7 months, 5 days
|
Re: Agar for a noob like me? [Re: c10h12n2o]
#26510030 - 02/29/20 04:56 PM (3 years, 10 months ago) |
|
|
Is your technique the T streak? I have sterile cutips. I'm just going to stick it and rub it on the print dry, and then streak my agar.
--------------------
|
psycho_nauticus
Stranger



Registered: 01/26/20
Posts: 779
Loc: Incognito on Cloud 9
Last seen: 7 months, 5 days
|
Re: Agar for a noob like me? [Re: A.k.a]
#26510048 - 02/29/20 05:02 PM (3 years, 10 months ago) |
|
|
So on my B+ transfers, whenever I dropped the little piece, it wasn't in the center, so I had moved it towards the center with my blade while doing the transfer. There are some white spots in that place where it originally feel and moved it. Is that probably just mycelium and spore from when I dropped it there. Or could be something different that I need to be concerned about?
--------------------
|
|