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OfflineDoctorDoggo
Experienced

Registered: 01/08/20
Posts: 6
Last seen: 3 years, 6 months
Piss or contam? Agar
    #26451445 - 01/25/20 08:44 AM (4 years, 22 days ago)

Hello humans,


TLDR: Is this piss or bacteria? D6 of growth




Further information:

I am a golden retriever. I am working on this project that started from dirty spores. What I currently have growing in my jars are on their 2nd (most) or 3rd (three jars) transfer from the original jar that grew from the print. I'm working with MEA. Temp 65-68 F. Growing in a dark closet.

Background: I inoculated and then had to go on a trip; two big colonies popped up on my original agar plate and a few days later a few more colonies grew. The first two big colonies seemed white and healthy up until about a week after growth (according to my agar baby sitter). The smaller colonies that grew after the two original colonies stayed white so I took many transfers from those and placed them in their own jars.

I picked three of those jars and made transfers from the leading edge of the mycelium (if possible) early. I can't remember if those colonies had turned yellow yet, I wasn't taking good notes then.

Bottom line: 6 days ago I took all my jars and made transfers. Some are on their second transfer (from original jar), some are on third. All of the newest jars (except MAYBE one) are now showing this yellow shit on them after I woke up this morning. They were all white up until last night, albeit a bit fuzzy.

I posted in general cultivation a few weeks ago with some shitty pictures I had from my original jar and the general consensus was it was bacteria.

Is this piss or bacteria that I need continue to clean up? I just don't get why this is following me around if I am taking from "clean" colonies that pop up several inches away from the central "contaminated" colony that is growing from the agar slice.

I'm going to make more agar jars soon and I have two I can use immediately. I'm new at growing and I'm kind of eager to get some fruits because I plan on medicating my depression with them. Dog's get depressed too.


Edited by DoctorDoggo (01/25/20 08:45 AM)


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Offlinesusurrador
Psychedelic Cowboy


Registered: 03/31/19
Posts: 1,446
Loc: SW US Flag
Last seen: 21 hours, 45 minutes
Re: Piss or contam? Agar [Re: DoctorDoggo]
    #26461235 - 01/30/20 11:22 PM (4 years, 17 days ago)

That dish definitely isn't right.

Would start more spores first. That way while you're trying to salvage what you have started... a backup plan is simmering in the event that things don't work out.

Then make a couple transfers from what appears to be what it should look like according to this post. Take a look at the agar pics in this post to compare. The details are very subtle. The discussion has a lot of relevant info as well.

https://www.shroomery.org/forums/showflat.php/Number/17231150#17231150

Also, I find it useful to keep a close eye on the dishes as they germinate and grow. If you walk away and don't see the daily changes... it will be more difficult to learn to distinguish what you want, from what you don't.

Lastly, mycelium benefits from light at all stages of growth. Don't steady keep your dishes in a dark closet or box. Ambient indoor light seems ok.


--------------------
"If it doesn't work, you can always hit him with it."



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