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Offlinethrowawaiting
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Best way for mycelium expansion from agar when doing pf tek
    #26461085 - 01/30/20 09:33 PM (3 years, 11 months ago)

Hey everyone. I know pf tek isn't the best or most recommended way of doing things and I wish I had just started with mono tub, because working with grains seems to be the way to go. However, I'm not sure if that is my best option since I'm doing pf tek and not going to switch since I've already made the initial investment to get everything going.

Here's my situation. I have made a clone of a nice looking mushroom I grew, transferred to another agar dish and I'm actually pretty happy with how the mycelium is looking there. Now I'm trying to find the best way I can expand this mycelium. I see a lot of recommendation for just throwing agar wedge into a grain jar, but this is usually in the context of bulk spawning. In my case, I will need to inoculate pf tek jars so I would need to make a liquid syringe.

I know I could do Liquid Culture, but I keep seeing people not recommend it too much due to not being able to see contams.

I was basically just wondering what you guys would do? Would you do a grain jar, then maybe do a slushy and put that in a syringe? Or would you just do a liquid culture? Or maybe there's a better option that I just don't know about.


Any help would be greatly appreciated!


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Offlineoldest hippie
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Re: Best way for mycelium expansion from agar when doing pf tek [Re: throwawaiting]
    #26461117 - 01/30/20 09:57 PM (3 years, 11 months ago)

Quote:

throwawaiting said:
Hey everyone. I know pf tek isn't the best or most recommended way of doing things and I wish I had just started with mono tub, because working with grains seems to be the way to go. However, I'm not sure if that is my best option since I'm doing pf tek and not going to switch since I've already made the initial investment to get everything going.

Here's my situation. I have made a clone of a nice looking mushroom I grew, transferred to another agar dish and I'm actually pretty happy with how the mycelium is looking there. Now I'm trying to find the best way I can expand this mycelium. I see a lot of recommendation for just throwing agar wedge into a grain jar, but this is usually in the context of bulk spawning. In my case, I will need to inoculate pf tek jars so I would need to make a liquid syringe.

I know I could do Liquid Culture, but I keep seeing people not recommend it too much due to not being able to see contams.

I was basically just wondering what you guys would do? Would you do a grain jar, then maybe do a slushy and put that in a syringe? Or would you just do a liquid culture? Or maybe there's a better option that I just don't know about.


Any help would be greatly appreciated!




This is my opinion only, but if this is your first grow experience, and you have everything that you need, do the PF Tek.
It is the easiest way to have a successful grow. I have never been disappointed with the results. After the first flush,
you can make your own spore prints, and clone your best specimens on agar. Good Luck


--------------------
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InvisibleSir Pentinite
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Re: Best way for mycelium expansion from agar when doing pf tek [Re: oldest hippie]
    #26461205 - 01/30/20 11:05 PM (3 years, 11 months ago)

And with spore prints you can make your own syringes and keep doing PF Tek. LC is probably not a good idea at this early stage.

If you wanted to step up to grain though, you're almost there.


--------------------
"I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'"
- Terrence McKenna


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Offlinethrowawaiting
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Re: Best way for mycelium expansion from agar when doing pf tek [Re: Sir Pentinite]
    #26461597 - 01/31/20 06:30 AM (3 years, 11 months ago)

I'm not sure if I was clear enough with my post. Let me try to reiterate. I currently have mycelium from a clone in agar dish. I want to expand this mycelium with the purpose of inoculating brf cakes. I'm wondering what the best way to accomplish that for my situation would be. From what I researched my options are either LC or grains


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OfflineCamera93
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Re: Best way for mycelium expansion from agar when doing pf tek [Re: throwawaiting] * 1
    #26461606 - 01/31/20 06:37 AM (3 years, 11 months ago)

Blenderless Liquid Inoculate

check this out
you could then use the LI to noc up your cakes


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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OfflineLogicaL ChaosM
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Re: Best way for mycelium expansion from agar when doing pf tek [Re: Camera93]
    #26461617 - 01/31/20 06:45 AM (3 years, 11 months ago)

Thats what I would do too. And it should be able to be sucked up into a spore syringe right? If not, u might need a bigger gauge syringe. And dont forget to make the holes on your jars bigger as well if u need that bigger gauge syringe.

Another alternative which would take a bit of work is to convert the lids on your PF Jars into gas-exchange ones using polyfill or Tyvek discs on top. U would bring your PF jars into your SAB, pour out the dry vermiculite, cut a slice off your colonized agar into the jar with the exposed PF material then seal up with a polyfill/Tyvek lid. Dont use a synthetic disc cause it will dry out the cake.


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Offlinethrowawaiting
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Re: Best way for mycelium expansion from agar when doing pf tek [Re: Camera93]
    #26461665 - 01/31/20 07:15 AM (3 years, 11 months ago)

Quote:

Camera93 said:
Blenderless Liquid Inoculate

check this out
you could then use the LI to noc up your cakes





Okay I'm a little confused. What is LI in this case? From what I saw, this is simply another way of inoculating grain jars with an agar dish. Which I would then have to find a way to get the mycelium in a syringe.

Or perhaps you mean only do step 1 of the guide and then use that to inoculate brf cakes. The problem I see with that is that the mycelium is not really growing, so I wonder how many brf cake jars I would be able to inoculate with just one agar dish. The nice thing about LCs is that you could expand that mycelium a lot more, right?


Edited by throwawaiting (01/31/20 07:18 AM)


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OfflineLogicaL ChaosM
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Re: Best way for mycelium expansion from agar when doing pf tek [Re: throwawaiting]
    #26461676 - 01/31/20 07:25 AM (3 years, 11 months ago)

One thing u can do is expand your one agar plate onto more agar plates to increase the amount of mycelium. Then do Munches blenderless LI Tek. A Liquid Inoculate is basically like a Liquid Culture, just not as dense. And this makes it easier to work with when using syringes.

And u can use it on PF Tek, just might need a slightly bigger gauge syringe.


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OfflineCamera93
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Re: Best way for mycelium expansion from agar when doing pf tek [Re: throwawaiting] * 1
    #26461679 - 01/31/20 07:28 AM (3 years, 11 months ago)

post some pics of your agar plate, if it needs more time..give it more time

The tek in a nutshell:
drop a colonized agar puck into sterilized water
shake the shit out of it
pour over your receiving jars, be it grain or brf cakes

LI = liquid Inoculate

pretty much just liquefied agar 

you could aspirate to a syringe, but that is only adding more vectors for contamination to find a way in

I would just pour into the jars though

yes LC is the only way to exponentially expand a culture


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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Offlinemycobuble
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Re: Best way for mycelium expansion from agar when doing pf tek [Re: Camera93]
    #26461693 - 01/31/20 07:44 AM (3 years, 11 months ago)

I asked a similar question recently
https://www.shroomery.org/forums/showflat.php/Number/26442809

Main suggestion was to create a syringe by sucking up mycelium off of agar with water.

Blenderless Liquid Inoculate looks great! Should we use less water in the PF tek to account for LI?


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OfflineNeowynd8
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Re: Best way for mycelium expansion from agar when doing pf tek [Re: Camera93]
    #26461700 - 01/31/20 07:52 AM (3 years, 11 months ago)

One Ms syringe is enough to do it all simultaneously. Makes a few few cakes and agar plates. You could even knock a grain jar and LC up with spores just for the hell of it.


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Offlinethrowawaiting
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Re: Best way for mycelium expansion from agar when doing pf tek [Re: LogicaL Chaos]
    #26461724 - 01/31/20 08:13 AM (3 years, 11 months ago)

Quote:

LogicaL Chaos said:
One thing u can do is expand your one agar plate onto more agar plates to increase the amount of mycelium. Then do Munches blenderless LI Tek. A Liquid Inoculate is basically like a Liquid Culture, just not as dense. And this makes it easier to work with when using syringes.

And u can use it on PF Tek, just might need a slightly bigger gauge syringe.




That is a good idea actually! Simply use more agar plates to expand the mycelium I have, and then just go straight from Agar -> BRF Cakes by using the LI Tek. I think I'm gonna go with that solution. Thanks a lot!!


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