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GenericBum
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I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML
#26460286 - 01/30/20 01:42 PM (3 years, 11 months ago) |
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Is it going to be ok? Corner looks green to me.
Edited by GenericBum (01/30/20 01:44 PM)
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bootedboy
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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: GenericBum]
#26460293 - 01/30/20 01:46 PM (3 years, 11 months ago) |
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yeah definitely doesn't look healthy
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GenericBum
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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: bootedboy]
#26460298 - 01/30/20 01:48 PM (3 years, 11 months ago) |
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What do I do and why did this happen?
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bootedboy
SpaceCowboy



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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: GenericBum]
#26460314 - 01/30/20 01:54 PM (3 years, 11 months ago) |
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Well I would need more information to diagnose the issue. I would spray that corner with alcohol or hydrogen peroxide and cut a large section of that corner out with a nice clean knife. Also if you can fruit it in a garage or outdoors somehow so as to not contaminate your grow space that would be much better.
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GenericBum
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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: bootedboy]
#26460354 - 01/30/20 02:13 PM (3 years, 11 months ago) |
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Took forever to colonize because I didn't shake up the syringe when innoculating. 3 3 pound grain bags colonized in around 66 degrees. Probably why it took so long. The substrate is just straight coco choir.
I think it dryed out because I cracked the lid during colonization based of the philosophy of bodhi's unmodified tub tech. https://www.shroomery.org/forums/showflat.php/Number/22337800/fpart/1/vc/1
I didn't mist it for along time after it was fully colonized because I assumed full colonization would mean that the whole tub from top to bottom would be covered in white mycelium and I assumed u aren't supposed to mist until it's pinning. I Also have been trying to heat it with a space heater because I thought it would help speed things up from the freezing temperatures It's been trying to survive in.
Edited by GenericBum (01/30/20 02:16 PM)
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WeavieWonder
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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: bootedboy] 2
#26460390 - 01/30/20 02:32 PM (3 years, 11 months ago) |
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Quote:
bootedboy said: Well I would need more information to diagnose the issue. I would spray that corner with alcohol or hydrogen peroxide and cut a large section of that corner out with a nice clean knife. Also if you can fruit it in a garage or outdoors somehow so as to not contaminate your grow space that would be much better.
Don't spray with iso or peroxide. And certainly don't cut anything off the sub. The area you find suspicious looks like bruising from lack of moisture. Give the entire sub a mist with water. If the sub is contaminated, it is going to stay that way no matter what you do. The best thing you the cultivator can do is just leave it the fuck alone and let it do its thing while maintaining proper conditions.
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naturalistic123



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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: GenericBum]
#26460399 - 01/30/20 02:36 PM (3 years, 11 months ago) |
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Id mist it
-------------------- Ὡς οὖν εἴπεν αὐτοῖς ὅτι Ἐγώ εἰμι, ἀπῆλθον εἰς τὰ ὀπίσω, καὶ ἔπεσον χαμαί.
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GenericBum
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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: naturalistic123]
#26460448 - 01/30/20 03:03 PM (3 years, 11 months ago) |
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Should I mist the sub directly? I thought it's only recommended to mist the walls of the tub? Is it ok to spray the sub directly in emergencies like this?
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bodhisatta 
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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: bootedboy] 1
#26460455 - 01/30/20 03:06 PM (3 years, 11 months ago) |
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Even not shook as long as spores actually make it in its fine. More spores doesn't mean faster. 4 small drops in four corners is faster than a whole syringe in one spot.
Premade bags of grain are fail
Inoculating grain directly with syringes is a guarantee to toss out at the least one in ten jars/bags on average.
With that said there's nothing wrong (yet) It looks dry. No green mold
Quote:
bootedboy said: Well I would need more information to diagnose the issue. I would spray that corner with alcohol or hydrogen peroxide and cut a large section of that corner out with a nice clean knife. Also if you can fruit it in a garage or outdoors somehow so as to not contaminate your grow space that would be much better.
Don't listen to this terrible advice.
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jbgtaa
extraterrestrial



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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: bodhisatta]
#26460467 - 01/30/20 03:12 PM (3 years, 11 months ago) |
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Don’t do anything until the contaminate really shows itself. I do see a spot that looks like sporulating mold but I could be wrong.
Best way to avoid this in the future, and to avoid waiting over 2 months for fruits? Start culturing on agar and practice your sterile technique. Using syringes for inoculation usually ends in less than desirable results.
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AyePlus
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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: bodhisatta]
#26460469 - 01/30/20 03:13 PM (3 years, 11 months ago) |
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Bruising from dry conditions. Mist lightly. Dont do anything drastic, for ms to pre sterilized grains I’m impressed but thats definitely a terrible methodology, even if it does work occasionally.
66will slow things down but a direct spCe heater will dry things out, point it away from tub in same room and try to keep at 70f
Also that sub looks shallow and uneven, in dry conditions a deeper substrate (~3”) will maintain humidity better, and an even surface will lead to even evaporation which in turn leads to even pinset.
How long since you mixed with bulk sub(spawned)
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A.k.a
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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: AyePlus]
#26460523 - 01/30/20 04:05 PM (3 years, 11 months ago) |
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A casing or a good top layer at spawn will help to stop it from drying out too. That was my main problem initially and doing a top layer/casing made a huge difference while I was learning how to dial my stuff in.
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GenericBum
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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: A.k.a]
#26462737 - 01/31/20 07:41 PM (3 years, 11 months ago) |
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So it seems like the general consensus is that it's just dry? I don't know how long it's been fully colonized because I haven't been keeping track of time but it feels like it's been at least 2 weeks. How long does it usually take for tubs to pin after full colonization?
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AyePlus
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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: GenericBum]
#26462745 - 01/31/20 07:45 PM (3 years, 11 months ago) |
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Yeah noone asked you that. When did you mix it with bulk sub. Should be pinning about 12-21 days from that day if its a regular cube, all is well and temps are 70-80f
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Sockadin



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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: AyePlus]
#26462760 - 01/31/20 07:56 PM (3 years, 11 months ago) |
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Definitely not mold. The sub is bruising. I never worried about misting walls, I'm not pinning plastic. Spray a healthy bit over the tub 1-2 ft at that imaginary cat laughing at you that is hovering over the sub. Spray straight and let the mist fall. Wait a day or two and fan that bitch out. You can use a casing layer, but I'm old school. I say spray and put a layer of plastic wrap lightly on top of the sub. Remove when you see nots.
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A.k.a
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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: Sockadin]
#26462798 - 01/31/20 08:32 PM (3 years, 11 months ago) |
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Man I hope it blows up with pins for you after waiting so long. It looks pretty healthy overall and definitely well colonized, I’m betting it goes quick once it gets mist and fresh air.
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GenericBum
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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: AyePlus]
#26462921 - 01/31/20 11:17 PM (3 years, 11 months ago) |
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Quote:
AyePlus said: Yeah noone asked you that. When did you mix it with bulk sub. Should be pinning about 12-21 days from that day if its a regular cube, all is well and temps are 70-80f
Idk how long it's been since I mixed it with the sub I haven't been keeping track of time. I only know how long it's been since I inoculated it because I have an email from Etsy saying my grain spawn arrived 74 days ago. My guess is that I mixed the grain in with the substrate about 5 weeks ago.
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AyePlus
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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: GenericBum]
#26462940 - 01/31/20 11:40 PM (3 years, 11 months ago) |
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Ambient temps? That is a very long time..
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GenericBum
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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: AyePlus]
#26462972 - 02/01/20 12:38 AM (3 years, 11 months ago) |
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Quote:
AyePlus said: Ambient temps? That is a very long time..
Probably around 65-70 degrees but for the past couple of weeks I have been running a space heater on it. I turn the space heater off at night because it gets to hot and drys it out when I'm not awake to constantly mist it.
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AyePlus
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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: GenericBum]
#26462976 - 02/01/20 12:49 AM (3 years, 11 months ago) |
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You want to keep the room warmer than that but no direct heat, point the space heater away from the tub just keep the room warm. 65 will basically triple or quadruple time to fruiting. You shouldn't need to mist every day or multiple times a day, a monotub is meant to be set and forgot until harvest typically. Unmodded tubs require a bit more fiddling but I wouldnt flip the lid or open at all until fruiting started. give it one good mist and put the lid all the way on if its drying out. Keep your temps above 68 and ideally somewhere around 72.
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GenericBum
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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: AyePlus]
#26467634 - 02/03/20 09:50 PM (3 years, 11 months ago) |
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Update Got my first pin!

Was worried that it would never happen. Hoping more start popping up. Why is it that monotubs always seem to form pins on the edges and corners of tubs first?
Anyways this is what the rest of the tub looks like right now.

Does it look healthy and should I be worried about it's yellow/brown color? Ive been accidentally overmisting it cause I got a new spray bottle and got a little overzealous. Got a flashlight app on my phone to look at it closer and discovered that I've probably been overmisting it the past couple days. I saw that it was sitting it pools of water when I lit it up. Does mycelium recover from flooding?
Edited by GenericBum (02/03/20 10:47 PM)
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Mr.Wizard
Harbinger of Hallucination



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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: GenericBum]
#26467734 - 02/03/20 11:26 PM (3 years, 11 months ago) |
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I unfortunately am not at this stage yet, but I wanted to link the below in case you haven't seen it, and it helps.
https://www.shroomery.org/forums/showflat.php/Number/23999053
EDIT: I didn't notice I was seeing the first post of the second page. Looks like you have folks that are way better than me helping ya already
-------------------- Tricks to the search bar: https://www.shroomery.org/forums/showflat.php/Number/24270830 Where to Start: https://www.shroomery.org/forums/showflat.php/Number/24420178/fpart/52#27623666 My easy to see modified no-pour: https://www.shroomery.org/forums/showflat.php/Number/26467702 I am so happy and grateful that we get to live in joyful abundance, while things get better, and better.
Edited by Mr.Wizard (02/03/20 11:29 PM)
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WeavieWonder
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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: Mr.Wizard]
#26467797 - 02/04/20 12:54 AM (3 years, 11 months ago) |
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It looks like you have almost mothered that tub to death. The best thing you can do at this point is just stop fucking with it.
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A.k.a
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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: WeavieWonder]
#26467934 - 02/04/20 06:09 AM (3 years, 11 months ago) |
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I’m not sure but that looks like a shitload of metabolites yeah??
I’ve seen people suck them up and save them, not sure I’ve ever seen them used but man now would be the time.
Op I can’t tell if the yellow is dry or pooled up liquid, if it’s wet it might help to soak it up with a paper towel. I’m not positive though it might need it there if it’s metabolites.
Congrats on that pin, hopefully more come soon. Have you looked at the bottom? Might be some hiding.
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jbgtaa
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Re: I've been waiting 73 days since inoculation and now I think my monotub might be contaminated. FML [Re: A.k.a]
#26469407 - 02/04/20 10:38 PM (3 years, 11 months ago) |
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You’re not going to get the flush you want big dog. Metabolites of that magnitude only happen with bacterial contamination present.
-------------------- If the thunder don't get ya, the lightning will. In another time's forgotten space, your eyes looked through your mother's face. Trade List Forever giving away prints. PM at anytime for a free print.
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