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Offlinethekickingbird
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Registered: 11/22/19
Posts: 26
Last seen: 2 years, 11 months
What did I do wrong??
    #26460106 - 01/30/20 11:51 AM (3 years, 11 months ago)

Hi all!!

Recently I get 2 spore prints (GT and Tampanensis), I live in Argentina and since it is too difficult to get those SP here I took special measures to maximize my success change. I worked with agar, following strict sterile procedures I made 10 soft PDYA plates:

  • 5 GT plates germinated perfect (showing white tomentose formations in 5 days)
  • 5 Tampanensis plates show no signals of mycelium neither contamination (19 days since inoculation).


I used a home made stainless steel inoculation tool, which I previously sterilized on my PC and later red hot flame sterilized between plates. I cooldown the tip by pitching the agar and then scratching inside the spore print, then streaking inside the petri plate.

In a second try I used the same steps, with the same plates (I think it is not the best option but no contams so far), but this time instead I used the scalpel to scratch the reminds of the SP until it get clean after inoculate the 5 plates again.

What did I do wrong?
The tampanensis SP was very light but one could see the spores on it.

Thnaks a lot in advance!


Edited by thekickingbird (01/30/20 11:53 AM)


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InvisibleDiego


Registered: 12/30/19
Posts: 346
Re: What did I do wrong?? [Re: thekickingbird]
    #26460116 - 01/30/20 12:00 PM (3 years, 11 months ago)

Maybe the print is old. Try hydrating it or use more spores


--------------------
Some do it for the income
But we do it for the outcome
Some of us are active
While others just let their mouths run - Chali 2na & Jurassic 5


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OfflineTheApprentice
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Registered: 09/25/11
Posts: 4,727
Last seen: 2 years, 1 month
Re: What did I do wrong?? [Re: Diego]
    #26460131 - 01/30/20 12:08 PM (3 years, 11 months ago)

Quote:

Diego said:
Maybe the print is old. Try hydrating it or use more spores





here is a thread I came across to help you germinate old spores.  I'm currently having the same problems with my whole spore print library :stoned:


STRO's FORCED HYDRATION FOR OLD SPORES


--------------------


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Offlinethekickingbird
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Registered: 11/22/19
Posts: 26
Last seen: 2 years, 11 months
Re: What did I do wrong?? [Re: TheApprentice]
    #26460405 - 01/30/20 02:43 PM (3 years, 11 months ago)

Thanks a lot for your advice!!!

Quote:

Diego said:
Maybe the print is old. Try hydrating it or use more spores



Quote:

TheApprentice said:
Quote:

Diego said:
Maybe the print is old. Try hydrating it or use more spores





here is a thread I came across to help you germinate old spores.  I'm currently having the same problems with my whole spore print library :stoned:


STRO's FORCED HYDRATION FOR OLD SPORES




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