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OfflineFilthyRogue
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Registered: 01/29/20
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Are my spores just bad?
    #26459146 - 01/29/20 08:45 PM (4 years, 2 hours ago)

So I ordered some spores and started some PF style jars. I used BRF and vermiculite as the substrate, pressure cooked them at 15 psi, used a glove box and a shit ton of lysol, wore a mask and gloves, and upon inoculation, placed microphone tape over the holes of the jars. It's been about a month, and I have very minimal signs of colonization, if that's what I'm even looking at. This is my second grow, and I followed the exact same method before, with which I had all 12 jars colonize completely in less than a month. The only variation I had made to the process was that I placed them in a tub that was being heated by a second tub underneath with water and an aquarium heater, which stayed consistently between 75 and 83 degrees. My only working theories are:

1. When I felt the jars, they were particularly warm in the tub, too hot maybe?

2. Upon flipping the jars, a mild amount of substrate seemed to crumble visibly in the jar, showing a mild amount of shrinkage, which leads me to believe that maybe all of the moisture has evaporated

3. The spores were poor.

It is important to note that I took the time to thoroughly shake the syringe to distribute the spores, and at a month in there is ZERO sign of contamination. Any and all ideas as to what could be the issue would be helpful, thanks



Edited by FilthyRogue (01/29/20 08:56 PM)


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OfflineSockadin
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Re: Are my spores just bad? [Re: FilthyRogue]
    #26459159 - 01/29/20 08:54 PM (4 years, 2 hours ago)

How long ago did you inoculate? ALso please do not use a tub in tub. Just put them on a shelf or in a closet and let them colonize. I have gotten a dud syringe before but it is very rare.


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OfflinecronicrFacebook
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Re: Are my spores just bad? [Re: FilthyRogue] * 1
    #26459160 - 01/29/20 08:55 PM (4 years, 2 hours ago)

wrong jars, ditch the incubator, lose the foil and try again.
edit the vendors name from your post


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OfflineFilthyRogue
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Re: Are my spores just bad? [Re: cronicr]
    #26459168 - 01/29/20 08:59 PM (4 years, 2 hours ago)

Just to clarify, what you see on the jars is the tape, not foil. And just so I understand, what's wrong with the jars? My last grow did fine with the tall 1/2 pint jars.

I inoculated on the 28th of December

Also, thanks for catching that, vendor is removed


Edited by FilthyRogue (01/29/20 09:01 PM)


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OfflineSockadin
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Re: Are my spores just bad? [Re: cronicr]
    #26459203 - 01/29/20 09:20 PM (4 years, 1 hour ago)

Quote:

cronicr said:
wrong jars, ditch the incubator, lose the foil Tape and try again.
edit the vendors name from your post




What cron is saying is that the dry vermiculite on the top of the jar acts as your filter and you do not need anything blocking the holes on top.

  Also any excess heat is bad. IF you can comfortably sleep in the room, than the room is the correct temperature to grow and fruit in.


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OfflineFilthyRogue
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Re: Are my spores just bad? [Re: Sockadin]
    #26459204 - 01/29/20 09:23 PM (4 years, 1 hour ago)

I see now, this was a big help, thanks guys


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OfflinecronicrFacebook
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Re: Are my spores just bad? [Re: FilthyRogue] * 1
    #26459301 - 01/29/20 10:32 PM (4 years, 33 minutes ago)

after a decade saying lose the foil is embedded in my auto correct :lol:


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OfflineFilthyRogue
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Re: Are my spores just bad? [Re: cronicr]
    #26459308 - 01/29/20 10:40 PM (4 years, 26 minutes ago)

That's hilarious

I did a control group with the substrate just to be sure of what contamination should look like, and this is what I got lol



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Invisiblegizmo1
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Re: Are my spores just bad? [Re: FilthyRogue]
    #26459327 - 01/29/20 11:02 PM (4 years, 3 minutes ago)

Be careful with that I'm pretty sure that pink mold is pretty damn dangerous. I'd toss that entire flask or pc before dumping.


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Edited by gizmo1 (01/29/20 11:03 PM)


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OfflineLogicaL ChaosM
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Re: Are my spores just bad? [Re: FilthyRogue]
    #26459354 - 01/29/20 11:32 PM (3 years, 11 months ago)

Looks like a science experiment gone wrong. :eek:

OP, u definitely want differenr jars. I find the wide-mouth 8oz "shorties" to be the best.

Also, your PF mixture was a bit too loose. Try gently compacting it down next time.


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OfflineYumyumyumyum
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Re: Are my spores just bad? [Re: gizmo1]
    #26459364 - 01/29/20 11:44 PM (3 years, 11 months ago)

I thought the inoculation was suppose to be in the dark? I did cakes in the dark they came out ok. I should have let them sit an extra week but that was a rookie mistake on my part. The tub is fine I did my jars the same kind of setup but darker and the jars did not have direct contact with the plastic surface. I elevated them just a touch. The temp looks right. Any sign is a good sign. Its the dead of winter in most parts. Good luck.


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Invisiblegizmo1
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Re: Are my spores just bad? [Re: Yumyumyumyum]
    #26459388 - 01/29/20 11:56 PM (3 years, 11 months ago)

Quote:

Yumyumyumyum said:
I thought the inoculation was suppose to be in the dark?



Might be kinda hard to inoculate in the dark. Lol.
Outdated info we now know that a 12/12 light cycle is beneficial to mycelium in all stages. Including COLONIZATION.  :thumbup:


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Offlinejbgtaa
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Re: Are my spores just bad? [Re: gizmo1]
    #26459390 - 01/29/20 11:59 PM (3 years, 11 months ago)

Just keep it all by a window. And start using agar to culture healthy mycelium, and then put that to BRF, or grain preferably.


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