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OfflineDurgin
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Registered: 02/23/19
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Do I spawn the last pint or not?
    #26459123 - 01/29/20 08:33 PM (3 years, 11 months ago)

(Originally posted to the LAGM 2020 thread, but posting here to get more feedback.)

So of the four B+ soft agar plates I dropped to wheat pints ten days ago, 3 are done and 1 is almost there:



My plan is to spawn them all to a single shoebox, and I'm debating whether to wait a couple days for the last jar to finish. It's like 95% colonized, but there's still a bunch on the top uncolonized:



I don't think it's contaminated, just slow, but sharing in case others see something I don't. These were inoculated with full 60mm soft agar T1 plates, which looked clean but not well organized before inoculation:



In hindsight, I should've labeled the plates and jars to be able to match them up, but didn't think about that at the time. My goal was to have keep a mix of genetics in the grow so I could get clones, and as such I wasn't concerned about any individual plates yet.

And that's also why I'm not just throwing out the last jar because it's a little slower. If there's reason to think the last jar is not clean and could screw up the rest, I'll toss it, but if it's just slow I'd like to give it a chance. I don't want to select those genes out just yet, especially since my guess is that speed differences are mostly due to differences in soft agar dispersion. (Two of the jars had plates melt like butter, and those colonized in under a week, but the other two were chunkier and those haven't been as fast.)

So what do you all think? Should I spawn the three finished pints now, or wait a couple days for the last to catch up?


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Offlinekushman88
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Registered: 10/20/13
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Re: Do I spawn the last pint or not? [Re: Durgin]
    #26459128 - 01/29/20 08:36 PM (3 years, 11 months ago)

Spawn now and transfer good agar growth but I’m still  new šŸ¤·šŸ¾ā€ā™‚ļø


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OfflineGrowtech
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Registered: 11/09/19
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Re: Do I spawn the last pint or not? [Re: Durgin]
    #26459130 - 01/29/20 08:37 PM (3 years, 11 months ago)

Let it catch up.  Ideally let it fully colonize, then wait another 4-5 days to be safe.  Spawning something that is partially colonized (even a little) is just asking for contamination to take hold.


--------------------
Mycolo.gyGenetics & yield tracking system


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Offlinekushman88
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Re: Do I spawn the last pint or not? [Re: Growtech]
    #26459176 - 01/29/20 09:06 PM (3 years, 11 months ago)

Sweet thanks man I was gonna wait at least a week before transferring


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OfflineSockadin
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Registered: 01/03/10
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Re: Do I spawn the last pint or not? [Re: kushman88]
    #26459206 - 01/29/20 09:23 PM (3 years, 11 months ago)

Looks pretty well done to me, but it also looks a little off.... I would spawn it in a separate container.


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