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OfflineShroombieNoom
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Registered: 10/19/19
Posts: 63
Loc: Cascadia
Last seen: 3 years, 10 months
Reviving dried cyans
    #26457796 - 01/28/20 11:29 PM (4 years, 22 hours ago)

So, I’ve seen a few posts from people documenting how they revived some dried cubes on agar and got them growing again. In those posts people did the normal cloning thing and split the stem to get a clean tissue sample.

I foraged some psilocybe cyanescens this past fall and, through newbie error, did not make proper spore prints to save for future growing. I am trying to get some outdoor patches started here, and while I’m waiting on a new spore syringe for cyans to arrive, I got to wondering if I could revive some of my dried stash from this fall.

So, a few days ago I put some small clippings of stems on some agar. Because cyan stems are very thin and brittle after drying, I couldn’t get clean samples from the insides of the stems. I just did my best to handle them carefully as I removed them from the jar where they’ve been hanging out the last few months.

One of the plates got obvious contamination within two days. But now three days since putting them on agar, two of them actually look kind of promising, though one more so than the other.

Here’s a pic of the two plates:



The right one obviously looks healthier than the left, but I’ll give the left one another few days to prove itself.

Not sure how far this will go, since there’s still a good chance for contamination to pop up, but I wanted to share this here for posterity’s sake, in case anyone else does a search to see if this is possible.

I’ll try to post updates as this progresses, too.


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Invisiblegizmo1
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Registered: 06/15/11
Posts: 3,831
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Re: Reviving dried cyans [Re: ShroombieNoom]
    #26457821 - 01/29/20 12:13 AM (4 years, 21 hours ago)

So I'm not too familiar with what cyan mycelium looks like but the right one is definitely fked and the left one looks bacterial as well maybe try a hot pour with the first one. I'd toss the 2nd one.


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OfflineRaccoon
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Registered: 04/08/19
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Re: Reviving dried cyans [Re: ShroombieNoom]
    #26457830 - 01/29/20 12:25 AM (4 years, 21 hours ago)

The right plate looks pretty bacterial to me. The left might have some cyan myc and probably something else. I'd transfer the left plate to see what happens.


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OfflineShroombieNoom
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Registered: 10/19/19
Posts: 63
Loc: Cascadia
Last seen: 3 years, 10 months
Re: Reviving dried cyans [Re: Raccoon]
    #26458134 - 01/29/20 08:09 AM (4 years, 14 hours ago)

Oh wow. Thanks! I’m new to agar. Obviously I didn’t recognize that the right one is bacterial... looking at it closely in person it looks like myc. But I definitely defer to y’all’s expertise.

I’ve read some posts in the wood lovers thread that cold-loving, wood-loving plates can be cleaned up in the fridge since the myc will keep growing but most bacteria will stall out. I’ll try that and report back if things work out.

Worst case my spores will arrive tomorrow and I can get going that way. The last spore syringe i ordered was totally unviable. The fine shroomery sponsor I ordered from is replacing it for me. :thumbup:


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