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Prietenul
Friend

Registered: 11/22/19
Posts: 379
Loc: Germination
Last seen: 3 years, 5 months
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Quote:
psycho_nauticus said: Should I take off all the foil on my jars before going in the SAB? Or I need to take them off and put them back on all inside the SAB?
I put them in SAB right after PCing with foil on top. When they're cool I spray and then take the foil off. Foil is just to prevent water from dripping onto them. It's not needed after PC anymore.
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vc77
incarnate


Registered: 06/27/04
Posts: 1,302
Loc: PNW US
Last seen: 3 years, 10 months
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Growing mushrooms is (and should be) easy
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Prietenul
Friend

Registered: 11/22/19
Posts: 379
Loc: Germination
Last seen: 3 years, 5 months
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Re: SAB misting? [Re: vc77]
#26455963 - 01/27/20 08:21 PM (4 years, 20 days ago) |
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Looking at all those threads in this forum, I'd say there are people who'd disagree. 
But hey, as said, what works for you, works for you.
Edited by Prietenul (01/27/20 08:22 PM)
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,755
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A couple years ago I noticed I was getting bacteria when misting. Been dry ever since. Also don't wipe my jars and don't clean my SAB (it will never really be clean anyway).
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 3 months, 8 days
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Quote:
Prietenul said:
Quote:
Sockadin said: I will Lysol the air in the SAB, but let be honest those things only work half the time. You put your hands in. You pull them out. I mean a still Air box is a lot of work.
Isn't that dangerous, especially if you keep your burner close to the SAB?
For working with spores you'd still need a SAB, right?
So let me be more clear. I will Lysol the sides and topinside of the SAB then I walk away for a while. Drink a beer smoke a cigarette and after 15 minuets I will go back clean all my tools and get to work. I have never had an issue with Lysol, but if it is flameable please don't do what I just said. For the last 6 years I have used my flow hood.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,395
Loc: where?
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Re: SAB misting? [Re: Sockadin] 1
#26456864 - 01/28/20 01:29 PM (4 years, 20 days ago) |
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If a sab is a lot of work then you are doing it wrong.
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psycho_nauticus
Stranger


Registered: 01/26/20
Posts: 779
Loc: Incognito on Cloud 9
Last seen: 7 months, 24 days
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Re: SAB misting? [Re: mushboy]
#26456951 - 01/28/20 02:21 PM (4 years, 20 days ago) |
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I think I'm just going to go with the basic wipe down of everything with iso and that's it. Everything else being said seems so overwhelming that it almost makes we just want to do it open air.
So far the hardest thing about my SAB is making the holes and filing them to make them smooth which I still need to do tonight. I'll probably do that while I PC.
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vc77
incarnate


Registered: 06/27/04
Posts: 1,302
Loc: PNW US
Last seen: 3 years, 10 months
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There ya go. Just focus on your technique and the SAB will do what it’s supposed to.
If you have 50$ and some time to spare I would recommend picking up a small Dremel tool from your local hardware store. Something like this https://www.homedepot.com/p/Dremel-200-Series-1-15-Amp-Dual-Speed-Corded-Rotary-Tool-Kit-with-15-Accessories-and-1-Attachment-200-1-15/202349458

I use it to make lids and all sorts of other projects for other shit I do.
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Drboomer
The lord magnificent



Registered: 09/22/19
Posts: 957
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Fuck it man next time I'm wiping the inside down, laying down a damp towel since I dont have racks yet and going in dry. I keep having bacteria issues. It's getting to the point that its pissing me off. I know my technique is on point. It may be the misting. I always lay an iso soaked towel on a pasty and work on top of that. My technique hasn't changed but my contamination rates have.
Anyone have links to good sab and agar videos? I've watched bods, munchs and mushboys.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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I use 70 iso and leave it dripping on the sides. I work my sab wet. Anything in the bottom isnt necessary. Its all about technique.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,395
Loc: where?
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Re: SAB misting? [Re: Drboomer] 1
#26457012 - 01/28/20 03:00 PM (4 years, 20 days ago) |
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Quote:
Drboomer said: I keep having bacteria issues..
When and where? Sometimes excessive bacteria can be from improper sterilization(not venting)
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Prietenul
Friend

Registered: 11/22/19
Posts: 379
Loc: Germination
Last seen: 3 years, 5 months
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Quote:
psycho_nauticus said: So far the hardest thing about my SAB is making the holes and filing them to make them smooth which I still need to do tonight. I'll probably do that while I PC.
I just took an old pot, put it on the stove upside down until it was hot, and then pressed it against the front of my SAB. Gave me some nice clean concentric armholes.
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,401
Loc: Over by your Mama's house
Last seen: 23 hours, 35 minutes
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Metal coffee cans work amazingly as well.
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 1 day, 8 hours
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I was having bacteria problems on plates and somebody told me to make sure to get the whole blade red hot not just the top half. Didn’t think that would make a lot of difference but turns out it did help a lot.
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LAGM2020     
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Drboomer
The lord magnificent



Registered: 09/22/19
Posts: 957
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Re: SAB misting? [Re: mushboy]
#26457146 - 01/28/20 04:03 PM (4 years, 20 days ago) |
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I do vent for sure set a 10 min timer. It usually shows up after the plate starts growing. A few satellite contams and often my plates start growing out hit a section and go aerial. I'm assuming it's an unseen spot of bacteria. The growth all around it stays uniform. Just that area goes aerial. I keep transferring away but it keeps happening. It's not all plates just maybe 20% I've even gone so far as to hold the scalpel by the very end. I think I'm going to try cutting nutes by 25% (munches pda recipie)and do my next few transfers dry. Maybe I'm pulling in an unseen water droplet in with my scalpel. It's not dripping wet but I get condensation in the box.
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Goombas69
Amateur Mycologist

Registered: 12/15/19
Posts: 15
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Quote:
LogicaL Chaos said: Misting with Iso makes a bomb... 
I wiped with 97% iso and then sprayed with Lysol and waited 30 min to sterilize my knife in the SAB. I blew myself up like a dumb-ass and had 1st and 2nd degree burns and no hair on my arms. It could have gone much worse.
Be careful out there...
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Prietenul
Friend

Registered: 11/22/19
Posts: 379
Loc: Germination
Last seen: 3 years, 5 months
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Yeah, most of the TEKs I read, tell you to never mist your SAB with anything flammable, be it Lysol or Iso or whatever.
That's why I don't do it. Pure and simple 
Lately I had great success with this "Biopsy Transfer":
https://www.shroomery.org/forums/showflat.php/Number/25133936
I don't think I'll ever do it differently, and now I have 200 scalpel blades, that I can use for cutting caps off of shrooms
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Just dont flame anything inside and you are fine. I use a bernzotorch and an exacto knife. I dont sit scalpals in 70 iso thats asking for a fire.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,395
Loc: where?
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How? 70% iso wont combust with room temp/cooled blades.
The risk is putting red hot blades(post flaming) into a confined space with iso vapor. But you know that.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: SAB misting? [Re: mushboy]
#26457579 - 01/28/20 08:12 PM (4 years, 19 days ago) |
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Let it settle before doing any work.
Never caught anything on fire other than a microwave with some coir. 
I leave the sides, top and bottom dripping with alc. you can kinda see it in this gif if you look closely. I spray it initially first. Close my sab holes with my cent covers and then open as i wipe down plates with an alc costed paper towel. By the time im doing work its been a few minutes since the alc has been sprayed. Flame outside and away from your sab. Ive brought s blazing hot blade into my sab thousands of times.
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