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Offlinecryptowizard
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Registered: 12/05/19
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Agar Plate Management Strategy?
    #26456671 - 01/28/20 11:21 AM (4 years, 19 days ago)

I'm curious how you all are using/storing/disposing of your agar plates, and have a few questions.

Assuming you have Germination plate, Transfer 1, Transfer 2. . . , Clone, master slants, etc.

What do you hang onto? Do you toss your germ plates after successful T1 plates? Are you only making backup slants after a successful harvest or along the way? If you go T1 to T2, are you doing anything with T1 besides storing or tossing it? What's your strategy and methodology for managing plates?

I assume if your taking 3 wedges from 1 germ plate you label/track them separately all the way through. If you went to T5 before fruiting, had a phenomenal yield, you're making a backup slant of T5 correct?

You grow out a clone on a plate, make a backup slant, and then continue to fruiting them, correct?


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Offlinesonoramo
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Re: Agar Plate Management Strategy? [Re: cryptowizard]
    #26456752 - 01/28/20 12:26 PM (4 years, 19 days ago)

I hang onto the germination plate until I'm confident that I have two uncontaminated transfer plates from the strains I want to keep. If a transfer plate is clean, I pretty much hang onto it until it isn't any good for transfer anymore. It might turn blue, or dry out or run out of agar. When I cut agar wedges, I try not to cut all the way through the agar, but rather scrape the mycelium off the first millimeter or so of surface. That seems to force the mycelium to make the leap onto its new medium more quickly (grain or agar). And, the mycelium left behind re-colonizes the freshly exposed agar.

I don't like slants! I tried buying tubes with threaded tops, and it was just too hard to manage the agar as it sets, too hard to inoculate the agar without touching the neck of the tube, too hard to see what I'm doing inside, too hard to see what's happening inside the tube, and too hard to retrieve a sample from inside without the scalpel or inoculation loop touching someplace on the tube I didn't want to contaminate. Small PP5 reusable plates are cheap and secure if wrapped on the side with parafilm or saran wrap.  Their tops and bottoms fit together much more securely than glass plates. These are easy to store in the refrigerator, easy to inoculate, easy to get samples out when needed.

I've sampled a wedge from BRF back to agar for storage, and this worked for me.

As for disposal, larger plates sometimes fruit. Even the mycelium is active and tastes pretty much like mushrooms. Assuming, that is, you didn't put anything disgusting in the agar medium.

Best way ever to back up your culture is to give it away!


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OfflinePrietenul
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Re: Agar Plate Management Strategy? [Re: sonoramo]
    #26456893 - 01/28/20 01:50 PM (4 years, 19 days ago)

Quote:

sonoramo said:

I don't like slants! I tried buying tubes with threaded tops, and it was just too hard to manage the agar as it sets, too hard to inoculate the agar without touching the neck of the tube, too hard to see what I'm doing inside, too hard to see what's happening inside the tube, and too hard to retrieve a sample from inside without the scalpel or inoculation loop touching someplace on the tube I didn't want to contaminate.




I have started to use PCable culture slants with distilled water. No need for agar, no need for wood or anything. I PC the culture tubes with distilled water in them, then scrape a tiny amount of myc from the agar plate I want to store long term, and drop it into the tube with distilled water. Finally screw the top onto the tube and seal with whatever you have.

There are some threads around that suggest you can keep the culture viable for many, many years in room temp.

https://www.shroomery.org/forums/showflat.php/Number/22554386

https://www.shroomery.org/forums/showflat.php/Number/22664891


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OfflineA.k.aM
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Re: Agar Plate Management Strategy? [Re: Prietenul]
    #26457105 - 01/28/20 03:43 PM (4 years, 19 days ago)

I’m relatively new to agar still but I’ve done prob 150 plates.

The main thing I just started doing is putting two T1s onto each plate and transferring as soon as it’s grown out like 1/4 inch.

At first I was putting each wedge on its own plate and letting it grow out but it takes too long and too many plates. It took me a little bit to realize T1s are pretty useless. Sometimes I’ll do two t2s on a plate as well and then go down to one transfer of the best looking growth when I’m ready to grow it out for grains.

I keep my germ/clone tissue plates just Incase something happens I can go back. Plus with germ plates you can always go back and get a different section with new genetics.


--------------------
LAGM2020


Edited by A.k.a (01/28/20 03:45 PM)


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Offlinecryptowizard
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Re: Agar Plate Management Strategy? [Re: Prietenul] * 1
    #26457135 - 01/28/20 03:58 PM (4 years, 19 days ago)

It's stunning to see how far the knowledge on this board has evolved since I was here over 10 years ago. Incubators, 86 degrees, fanning and misting. Now we have everything at room temperature and TEK that is almost maintenance free.

Thanks for posting, this is such a better method. Now I have 60 something test tubes that I have no use for. . .


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OfflineA.k.aM
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Re: Agar Plate Management Strategy? [Re: cryptowizard]
    #26457198 - 01/28/20 04:32 PM (4 years, 19 days ago)

I think tube slants are still the best long term storage option.

Lc is awesome but one mistake and the whole thing is dirty. If I had cultures I wanted to hold onto indefinitely I’d use the slants with the popsicle stick.


--------------------
LAGM2020


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OfflinePrietenul
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Re: Agar Plate Management Strategy? [Re: cryptowizard]
    #26457213 - 01/28/20 04:42 PM (4 years, 19 days ago)

Quote:

cryptowizard said:
Thanks for posting, this is such a better method. Now I have 60 something test tubes that I have no use for. . .




Well you could buy some caps and use those as culture tubes. You won't have the nice screw cap but if you seal it right, there shouldn't be an issue I think.


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OfflinePrietenul
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Re: Agar Plate Management Strategy? [Re: A.k.a]
    #26457222 - 01/28/20 04:44 PM (4 years, 19 days ago)

Quote:

A.k.a said:
I think tube slants are still the best long term storage option.

Lc is awesome but one mistake and the whole thing is dirty. If I had cultures I wanted to hold onto indefinitely I’d use the slants with the popsicle stick.




I did not mean LC. I was talking about slants that have screw caps that you can PC with distilled water in them. You scrape some myc from your plate into them, seal them and keep them wherever you want. As the distilled water has no nutes in it, contams (and also myc) have nothing to feed on. It will put the myc in a dormant state until you go and drop some of that onto agar.


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