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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Microwaving agar [Re: Sockadin]
#26457027 - 01/28/20 03:08 PM (4 years, 1 day ago) |
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Why? Lol.
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FrankRhizo
Interdimensional Travel Agent

Registered: 11/28/17
Posts: 295
Loc: Subterranean
Last seen: 6 months, 3 days
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Re: Microwaving agar [Re: gizmo1]
#26457099 - 01/28/20 03:41 PM (4 years, 1 day ago) |
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Quote:
gizmo1 said: I dont know. When you say the bottle was filled to the neck what exactly do you mean can you post a pic sounds like overfilling. I wouldn't be scared of the microwave I've done it a bunch of times and am yet to see any contams because of it. I always the the cap loosened. I also freeze my agar and go straight from microwave to the freezer most of the time. I prefer to wait but you know how life is sometimes I'm just too busy. Have some faith in the process either that or just prep and pressure cook your agar only when you are ready to use it. Any reason why you chose 250ml bottles? I have them also but my 500ml ones dont fit in the microwave and I use less agar now that I switched to 60mm petris.
I also have 60mm petris, 10 a sleeve. The 250 does 2 sleeves perfectly. I'm just trying to use my time, money, and energy efficiently. Instead of 4 PC sessions I could do 1. I don't have boil over problems cause I stick the 250s in a wide mouth pint jar.
Edited by FrankRhizo (01/28/20 03:43 PM)
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FrankRhizo
Interdimensional Travel Agent

Registered: 11/28/17
Posts: 295
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Last seen: 6 months, 3 days
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Edited by FrankRhizo (01/28/20 03:46 PM)
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Yea that's fine it's not overfilled at all. Like I said just have faith in the process crack the lid and microwave it. I'm sure it'll be fine I do it all the time then walk with the hot bottle across a dirty house with dirty carpet and mold. Straight into my lab where I let it cool in a SAB.
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Re: Microwaving agar [Re: gizmo1]
#26457293 - 01/28/20 05:35 PM (4 years, 1 day ago) |
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Cause you know me, we been growing together for years and I don't know what the fuck im doing after 10 years on this site.
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Microwaving agar [Re: Sockadin]
#26457312 - 01/28/20 05:46 PM (4 years, 1 day ago) |
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Quote:
Sockadin said: Cause you know me, we been growing together for years and I don't know what the fuck im doing after 10 years on this site.
Nothing wrong with 100mm petris though I like them I put 3 transfer on a single plate.
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345536345
Pan parent!



Registered: 08/13/17
Posts: 268
Last seen: 2 years, 3 months
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Re: Microwaving agar [Re: Sockadin]
#26457395 - 01/28/20 06:34 PM (4 years, 1 day ago) |
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Quote:
Sockadin said: I just got my case 500 of 100mm Pettis and now I'm like 
I actually prefer the 100mm dishes. You can drop three tissue samples if you wish on a plate and have room to see decent growth. If you drop one wedge on you can let it run for a solid week before having to transfer. I appreciate the extra flexibility and time if I need it. I do 400ml batches of agar which equates to 10 100mm dishes poured relatively thick (my preference) with a few ml to spare. Agar is cheap.
For your water bath question above you absolutely can. Just need to get it above say 190 or so until the agar fully melts and then drop it down to pouring temp. I have a sous vide stick that I love for that reason exactly. I don't need to time it just right. Just stick it in the water bath and it's always ready at the perfect temp when I want it. Labs employ the same approach as I understand it.
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Thats dope. I do not have a Sous Vide stick, but I also love to cook. Might be worth the investment. I just watched an episode of Munchies with matty matheson and I thought I remember he used one for making some crazy shit.
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Water baths work fine but take for fucking ever to melt the agar and I'm impatient so I go with the microwave.
Quote:
Sockadin said: Thats dope. I do not have a Sous Vide stick, but I also love to cook. Might be worth the investment. I just watched an episode of Munchies with matty matheson and I thought I remember he used one for making some crazy shit.
I want one for cooking a perfect steak.
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345536345
Pan parent!



Registered: 08/13/17
Posts: 268
Last seen: 2 years, 3 months
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Re: Microwaving agar [Re: gizmo1]
#26457454 - 01/28/20 07:08 PM (4 years, 1 day ago) |
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LOL! Yeah I usually microwave the agar to melt and then put into the water bath to bring down to, and hold temp for as long as I need. This way I go about my day and then when I'm ready I'm good to go.
It's also perfect for pasteurizing casing without having to babysit the stove.
In the meantime it's killer for getting meats perfectly cooked. I'm getting hungry now just thinking about it!
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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I dont own a microwave, you know..... Cancer and shit.
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