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murderlabz
RIP Stoneman


Registered: 05/18/19
Posts: 551
Loc: The Multiverse
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Re: Let's grow some mold [Re: gizmo1]
#26453423 - 01/26/20 12:04 PM (4 years, 22 days ago) |
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Quote:
gizmo1 said: I been thinking about buying some gaskets for my plastic lids.
Like these?
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gizmo1



Registered: 06/15/11
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Loc: FREEDOM
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Yup
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murderlabz
RIP Stoneman


Registered: 05/18/19
Posts: 551
Loc: The Multiverse
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Re: Let's grow some mold [Re: gizmo1]
#26453451 - 01/26/20 12:13 PM (4 years, 22 days ago) |
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mushboy
modboy



Registered: 04/24/05
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Quote:
murderlabz said:
Quote:
gizmo1 said: Yup
12 for 10 USD isn't bad.
https://www.amazon.com/Unique-Reusable-Silicone-Plastic-Gaskets/dp/B06XPH8QN3/ref=sr_1_1?keywords=Silicone%2BJar%2BRim%2BSeals&qid=1580065586&sr=8-1&th=1
They can be had a little cheaper I think there is a 48 pack of them for like 15 on there. I'm just a chronic procrastinator or else I'd have some already.
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PrimalSoup
hyperspatial illuminations



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Re: Let's grow some mold [Re: gizmo1] 1
#26456103 - 01/27/20 10:43 PM (4 years, 20 days ago) |
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By god a mold thread? How did I miss this?
Gonna have to put my hazmat suit on to decant this fucker, which is already heading out the door:

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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
Edited by PrimalSoup (01/28/20 10:41 AM)
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cronicr



Registered: 08/07/11
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Loc: Van Isle
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Yeah I want to say it was a bad set of cultures but at the same time they are doing great on straw , oh well tomorrow is another day
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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newtomyc
enthusiast


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Re: Let's grow some mold [Re: cronicr]
#26456415 - 01/28/20 08:00 AM (4 years, 20 days ago) |
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Got to join this thread.
I have had nothing but Trench in the last 15 tubs I have spawned. Very frustrating.
-------------------- JJ Draw unto others as they have been drawn to you.... WSP
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cronicr



Registered: 08/07/11
Posts: 61,436
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Re: Let's grow some mold [Re: newtomyc] 1
#26456484 - 01/28/20 09:04 AM (4 years, 20 days ago) |
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it's just a part of the game man, can go 100 tubs without seeing it then lose 50 in a row.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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PrimalSoup
hyperspatial illuminations



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Re: Let's grow some mold [Re: cronicr] 1
#26456616 - 01/28/20 10:47 AM (4 years, 20 days ago) |
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Here's my suggestion, cleaned up a bit from total obscurity:
That it pops up right after knotting says to me it's an opportunistic parasite and it knows just when to attack the myc... Like the sneaky MF it is. You might try just prepping a tub without adding any spawn to see what happens over time.
That'd be the control.
I suspect though that the germination of the parasite is triggered by environmental conditions which could be something the myc secretes, in which case it just lies dormant until spawn comes along. IOW total doom once it gets a toehold. I've seen that stuff before.
Straw was never too good to me but I probably was prepping it wrong. I couldn't get the stalks short enough with the lawnmower and various contams came out from in there somewhere. OTOH I could get it for free off of road spills. 
But if it's really shredded and pasteurized right it works for edible producers.
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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R.I.P.Zappa
Myco Melyco


Registered: 03/30/18
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Been enjoying my time with just cultures and a few test runs. 3rd and final flush of "not so" rusty whytes from last falls contaminated oat spawn that I just topped off and let roll. Metobolic turd blob hung out the whole ride but seeing spots of mr. green showing up so shes tossed.

Back to WBS with PESH plate pin clone. Nice to see some canopies again instead of

And some plates with 10g agar 13g DME (to slow it down a bit) 1% GW and a 1/2 a gram of wine tannin to 600ml. Upping the tannin content to see if it will hinder cube myc growth and seems to have killed the smallest 4th transefers on the two PESH plates. Far left is RW just colonizing like a boss compared to the PESH.  Gonna swab some bacteria I saved on a blank tomorrow and see how it performs against the tannin. Looking promising so far since cube myc will colonize wood which is full of tannins but gotta see how good it is as a natural anti bacterial agent outside of wine making.
  
Abstract Tannin toxicity for fungi, bacteria and yeasts is reviewed and compared to toxicity of related lower molecular weight phenols. The dependence of toxicity on tannin structure is examined. The different mechanisms proposed so far to explain tannin antimicrobial activity include inhibition of extracellular microbial enzymes, deprivation of the substrates required for microbial growth or direct action on microbial metabolism through inhibition of oxidative phosphorylation. A further mechanism involving iron deprivation is proposed. Many microorganisms can overcome plant defences based on tannins. They may detoxify tannins through synthesis of tannin- complexing polymers, oxidation, tannin biodegradation or synthesis of siderophores.
-------------------- -The heaviest thing one will ever carry is a thought- -"Like a Blind man In an orgy you gotta feel things out.".- -When we agree about our hallucinations, we call it “reality".- -If you defy authority because your told to, that's no better than blindly trusting authority.- psychonautwiki.org How it should & shouldn't look - NEW CULTIVATORS GUIDE BOD's Easy AF OAT prep tek. Principles of mushroom growing for beginners
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Psychgro
Mr. Stranger


Registered: 12/21/19
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Quote:
ShamanBoof said: Wtf! I thought mold was easy to kill by heat Remember this jar I shared a picture of
 I PCed for 1 hour to reuse the jar then decided to crumble the grains and PC for about 2 hours. I did not touch the jar after it's been sterilized for the second time, to my surprise one week later green is back!

Could it be your lid? Aren’t those the lids with minimal or no threads?
-------------------- Looking to learn all about it
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newtomyc
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Re: Let's grow some mold [Re: Psychgro]
#26471517 - 02/06/20 06:01 AM (4 years, 11 days ago) |
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I have some nice lime green spot on my last tub.

-------------------- JJ Draw unto others as they have been drawn to you.... WSP
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RhYzo
D1g1t@l Dw3ll3r



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Re: Let's grow some mold [Re: newtomyc]
#26471737 - 02/06/20 09:26 AM (4 years, 11 days ago) |
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This thread makes me itch!!! Gah!
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hummingbird

Registered: 06/29/14
Posts: 2,134
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Re: Let's grow some mold [Re: RhYzo]
#26504257 - 02/26/20 12:59 AM (3 years, 11 months ago) |
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Alright, I grew some mold...

Forgot about this one off in the corner of the basement for awhile. Came back to a full canopy of green, and thought about this thread...
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footpath
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Registered: 07/16/19
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Now, that's a green thumb.
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Inthepit
Aum Mani Padme Hum



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Re: Let's grow some mold [Re: footpath]
#26504422 - 02/26/20 06:39 AM (3 years, 11 months ago) |
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I can't find the reference but.
Quote:
Coir - I like to put mine in the oven in turkey trays at 250-300f for a couple hours.
Going over ~170 F? Would kill everything right? And, from what I read somewhere, that would leave an open door to contam. The point was not to kill off everything, just some. Sorry to be so vague, I'm lost without the reference.
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footpath
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Re: Let's grow some mold [Re: Inthepit] 1
#26504434 - 02/26/20 07:06 AM (3 years, 11 months ago) |
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I don't think it matters with coir. Some people sterilize it, some people pasteurize it (usually improperly), some people hydrate with cold water and roll with it right away. I have heard that cubes like cooked coir better, but I dunno how strongly that has been backed up with evidence. I'd look to cron for the answer on that.
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Inthepit
Aum Mani Padme Hum



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Re: Let's grow some mold [Re: footpath]
#26504539 - 02/26/20 09:02 AM (3 years, 11 months ago) |
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I'm still getting contam in a few virgin plates.  SAB, rack, damp towel, inside wiped with Lysol wipe, start at bottom of 10 plate stack. Gloves, arms, media bottle wiped with alcohol. I don't even tilt the media bottle back all the way between pours. Just started pouring less and swirling to cover the bottom c10 style. These contams are always starting at the edge. I didn't wipe the threads after removing the cap. Poured at 113~112F, Rx: 4g agar, 7g potato, 50% Oat broth, food color, for 500mL
Edited by Inthepit (02/26/20 11:27 AM)
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Let's grow some mold [Re: Inthepit]
#26504788 - 02/26/20 12:23 PM (3 years, 11 months ago) |
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I've been losing about 30% of my jars that have the white plastic lids, and when the jars don't fail the tubs spawned with them do. Trich.
No leak ball lids haven't triched so it must be getting in under the lid.
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