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psycho_nauticus
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SAB misting?
#26455740 - 01/27/20 06:27 PM (4 years, 20 days ago) |
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Do you have to mist all the walls with water of your SAB inside before inoculating? It seems like you could accidentally ruin your jars and everything if it happens to get on it. I was planning on cleaning the inside and outside with 70% isopropyl alcohol. Do you guys mist the walls with water after that? Is that a necessity?
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Northman
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I mist everything with 5% bleach water. Mist the walls and the air really well. The point is for the water mist to drop bringing down anything airborne with it.
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Prietenul
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I usually mist before putting stuff in, then wipe the surface on which I'm working with Iso, wipe my jars and stuff and put them in.
Sometimes stuff is already inside the SAB (e.g. Agar bottle cooling or fresh poured petris), so I put an Iso soaked paper towel over them and then mist the SAB.
I just think it's not so good to mist your airbornes onto your jars and dishes... but someone else might have a different opinion.
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Northman
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I should have gone into further detail, apologies. I will typically already have my stuff in there, however I ALWAYS wipe everything down with iso again after misting and again before using whatever it is I may be using.
For example when doing agar transfers, my petris and scalpel and new petris will all already be in there. I mist and let the air settle. I then will wipe down the colonized petri and the new petri with iso soaked paper towels. I then wipe my scalpel and flame sterilize. I will then proceed to do my transfer. When finished I wipe everything again before moving to the next transfer.
It might be overkill but I have prefer to be cautious and not waste.
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LogicaL Chaos
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I mist with water first than with iso propol. There is a draw back, water drippin from the ceiling of the SAB. That's an issue. Not sure what to do with that....
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Prietenul
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Quote:
Northman said: I should have gone into further detail, apologies. I will typically already have my stuff in there, however I ALWAYS wipe everything down with iso again after misting and again before using whatever it is I may be using.
For example when doing agar transfers, my petris and scalpel and new petris will all already be in there. I mist and let the air settle. I then will wipe down the colonized petri and the new petri with iso soaked paper towels. I then wipe my scalpel and flame sterilize. I will then proceed to do my transfer. When finished I wipe everything again before moving to the next transfer.
It might be overkill but I have prefer to be cautious and not waste.
As I'm relatively new to this, I might be wrong (and corrected fast for sure ) but if you have contaminants in the air and "wash" them onto your dishes, it could be that you don't catch them all by wiping again after the mist.
Also, e.g, you have your petris upside down (agar on top) lying in your SAB. If you mist then, the contams will fall into the slits between the lids and the bottom of your dishes. You can't wipe with Iso there...
So for me the risk of contaminating stuff would be to high. I'd always either cover my stuff with paper towels or put it in after misting. Just my 2ct
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Prietenul
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Quote:
LogicaL Chaos said: I mist with water first than with iso propol. There is a draw back, water drippin from the ceiling of the SAB. That's an issue. Not sure what to do with that....
Why not just wipe it away with an Iso soaked paper towel?
Edit: Misting with Iso makes a bomb...
Edited by Prietenul (01/27/20 07:24 PM)
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LogicaL Chaos
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Hmmm....why didn't I think of that
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Northman
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I've never had water drop from the ceiling of the sab before. I mist enough to thoroughly saturate the air but not enough for water to collect at the top.
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Prietenul
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Quote:
Northman said: I've never had water drop from the ceiling of the sab before. I mist enough to thoroughly saturate the air but not enough for water to collect at the top.
Hm, if I make two-three pumps, I have droplets on the ceiling
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Northman
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I keep Pettis right side up in the sab and they are wrapped with parafilm/syran until I am ready to use them. I simply wipe them down, remove the film, wipe again and get to work.
This is just what works for me. Ideally I'd rather be working with a flow hood and not stress the sab. But in my many years of working with agar in a sab when I haven't had access to a flow hood, I've rarely had any contams.
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A.k.a
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Re: SAB misting? [Re: Northman] 1
#26455853 - 01/27/20 07:30 PM (4 years, 20 days ago) |
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I never noticed water on the ceiling until one time I did a transfer and the second I put the lid back on a big drop landed square on the middle.
I spray air cleaner in first just cuz I have it then wipe ceiling and walls with Lysol wipes or iso towel.
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Sir Pentinite
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Re: SAB misting? [Re: A.k.a]
#26455863 - 01/27/20 07:36 PM (4 years, 20 days ago) |
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I don't bother spraying anything.
-------------------- "I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'" - Terrence McKenna
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A.k.a
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You been doing it a while?
I started out wiping and spraying everything but as time goes on I’m doing less and less.
Then you get to the point where you’re running dry SAB and not wearing gloves lol.
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LAGM2020     
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Prietenul
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Quote:
Northman said: I keep Pettis right side up in the sab and they are wrapped with parafilm/syran until I am ready to use them. I simply wipe them down, remove the film, wipe again and get to work.
This is just what works for me. Ideally I'd rather be working with a flow hood and not stress the sab. But in my many years of working with agar in a sab when I haven't had access to a flow hood, I've rarely had any contams.
As I have occasional condensation on the lids, I always keep them with the agar on top. I guess this is how I got used to it. Write on the back, keep them upside down, put them in after misting.
Of course, if it works for you, it works. That's the most important thing.
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Prietenul
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Re: SAB misting? [Re: A.k.a]
#26455877 - 01/27/20 07:42 PM (4 years, 20 days ago) |
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Quote:
A.k.a said: You been doing it a while?
I started out wiping and spraying everything but as time goes on I’m doing less and less.
Then you get to the point where you’re running dry SAB and not wearing gloves lol.
Yeah, until you hit contamination. Then you take care more again, until it lessens and lessens again. Live is mostly a (sine-)wave.
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Sockadin



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I will Lysol the air in the SAB, but let be honest those things only work half the time. You put your hands in. You pull them out. I mean a still Air box is a lot of work.
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Prietenul
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Quote:
Sockadin said: I will Lysol the air in the SAB, but let be honest those things only work half the time. You put your hands in. You pull them out. I mean a still Air box is a lot of work.
Isn't that dangerous, especially if you keep your burner close to the SAB?
For working with spores you'd still need a SAB, right?
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psycho_nauticus
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I was thinking of misting before I put everything in, I just didn't know if it would be a problem opening the SAB back up after misting with water to put my stuff in? I have some jars and a syringe, no agar.
I'm going to wipe down with iso, but then mist with water. I feel like misting inside with everything already inside could be a problem because there is a higher risk of something getting on my items correct? With it being my first time doing bulk and a SAB I don't want to make the mistake of something misting or dropping on my stuff.
So opening the SAB back up after misting is okay then?
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vc77
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Re: SAB misting? [Re: Sockadin] 2
#26455899 - 01/27/20 07:55 PM (4 years, 20 days ago) |
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Why mist when you don’t have to? Trust in gravity, your SAB works for a reason. I don’t wipe down my SAB, I don’t mist anything, I clean my SAB maybe once a month, maybe not even. It’s as easy as wiping down whatever you load into your dry-ass SAB and wiping your gloves down here and there as you work. Focus on refining your technique, hand efficiency and finesse.
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