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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26455855 - 01/27/20 07:31 PM (4 years, 21 days ago)

Yeah that looks thicker. I like those quarter pint jars you are using. I do prefer the larger though. Also dont be afraid to let that plate grow 80-90% before you LI it.


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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26455887 - 01/27/20 07:47 PM (4 years, 21 days ago)

Roger all that. I scheduled it a few days ago, but my MS from spores (4th transfer) is slow growing. Maybe thats just how it is, or maybe my soft. sweet agar does't give it much reason to hurry and colonize the plate.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Invisiblemurderlabz
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26456005 - 01/27/20 08:43 PM (4 years, 21 days ago)

:popcorn::heart:


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Re: EAT LIQUID INOCULATES™ [Re: murderlabz]
    #26462064 - 01/31/20 12:28 PM (4 years, 17 days ago)



More of my plates after a few more days.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Edited by tedoro (02/01/20 11:18 AM)


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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26462789 - 01/31/20 08:20 PM (4 years, 17 days ago)

any of you guys know what those weird blank "sectors" are?  Im thinking its a contam but not sure what.  it seems tricky to transfer away from sometimes.  always seems to pop up on ms from time to time


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Re: EAT LIQUID INOCULATES™ [Re: TedsDead]
    #26462794 - 01/31/20 08:27 PM (4 years, 17 days ago)

I’m wondering the same thing. I’ve only seen it recently when I started using spores.

This is a sagari plate I made, myc is running the whole thing down to the bottom. Should be perfect but I’m surprised it wasn’t mentioned at all in the sagari/li threads.



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Re: EAT LIQUID INOCULATES™ [Re: A.k.a]
    #26462796 - 01/31/20 08:30 PM (4 years, 17 days ago)

yeah, that what im saying I always see it when starting new spores.  you can get away from it easily enough but why wont the myc touch those spots?


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weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: TedsDead]
    #26463205 - 02/01/20 08:14 AM (4 years, 16 days ago)

I actually thought tedoro transferred slices out in those areas.


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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26463279 - 02/01/20 09:18 AM (4 years, 16 days ago)

Eat you never seen that before??

It’s for sure something with the spores I did a bunch of clones prior to LAGM and never encountered that.

Then my germ and T1s for pesa and aa both look like that.


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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: A.k.a]
    #26463309 - 02/01/20 09:39 AM (4 years, 16 days ago)

Ive seen it maybe two times randomly. Nothing consistent like that.


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InvisibleTedsDead
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26463337 - 02/01/20 09:57 AM (4 years, 16 days ago)

I see it quite often on ms plates.  something from unclean prints I imagine but I really dont know.  I thought it was just charactreristic of a certain variety for a while but once I started seeing it on other ms plates it occurred to me that all of the plates of said variety had also been ms.  I mustve started like 15 different varieties this month and saw it on alot of the plates across different varieties


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weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
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Re: EAT LIQUID INOCULATES™ [Re: TedsDead]
    #26463467 - 02/01/20 11:17 AM (4 years, 16 days ago)

Well I'm not going to use them (I used their brothers on a few bags, thats risky enough) I made transfers from them today.

Those bags from said funny looking red plates..

Day 5



I could barely see any leap-off at day 3... but then it explodes.

Left bag is at 47% percent water content. Right one is at 49% and was boiled just to the point the wheat berries were bursting and when I drained them, it was a little starchy and continued to dribble for a long time. Seems like a little extra water and a little starch on the grains speeds things. But I don't think the Karo I added sped leap-off. I'm going to stick with MEA for now.

I'm really wondering if letting the syringes sit at room temp for three days to recover might speed the recovery. That blender is brutal. (but so is shaking the bags for 2 whole minutes)


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26463524 - 02/01/20 11:53 AM (4 years, 16 days ago)

i mean, you might as well consider those 3 days part of your recovery time as well... right?


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: TedsDead]
    #26463603 - 02/01/20 12:33 PM (4 years, 16 days ago)

Tedoro do you think the thicker LI solution helped?

Did you pour more LI into the one on the right side?


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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26463734 - 02/01/20 01:50 PM (4 years, 16 days ago)

I'm trying to have a workflow that is fast. I don't mind doing the syringes 3 days earlier.... also, I want grains to be uncolonized for as little time after the PC'ing as possible.

I squirted the same amount in both bags. (1/2 agar plate in each)(I did eight bags total, between 47%-49%) and its the longer cooking, more water that sped the growth...... but Stamets recommends 47% for rye with my size bags.... so I kinda feel like at some point I'll be getting too wet for a large bag. My gut thought is wetter goes faster at a cost of being more bacteria prone. To a point, then too wet slows it all down.

Definitely the thicker poured plates helped. I didn't even let the plate grow out much, and every single little piece of agar grew mycelia. I don't think the Karo did much. 1 part agar 1 part malt 2 parts Karo.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Edited by tedoro (02/01/20 03:02 PM)


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Offlinetedoro
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26463745 - 02/01/20 01:58 PM (4 years, 16 days ago)

Hey eats. Thanks so much for this thread. I'm really excited that my silly refusal to get a flowhood has a working solution. I'm growing out really great bags and I just realized that now that I have pre-sealed PC'ing down.... I can do 8 smaller, 2 quart bags in my presto... to grow out shoeboxes that are knocked from plates using LI, to test out strains. Its pretty sweet. I'm even soon going to start reusing bags.... considering I used to PC for 4.5 hours, I figure two, 2.5 hour runs ought to be okay.

Anyway, I'm rambling... thanks for this and all the help.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26463922 - 02/01/20 03:55 PM (4 years, 16 days ago)

I actually used to reuse bags ehen i used to do them. Just reseal with the sealer. Good luck. Its all snout finding that perfect recipe for the variables you are using. I also think jars colonize a little faster because when you shake them, you almost hit all sides of the grain with the LI. I always find the thicker solutions do the best. I just use karo in my pasty plates and noticed they leap off much faster than other normal agar plates ive used.


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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26463971 - 02/01/20 04:21 PM (4 years, 16 days ago)

Quote:

eatyualive said:
I actually used to reuse bags ehen i used to do them. Just reseal with the sealer. Good luck. Its all snout finding that perfect recipe for the variables you are using. I also think jars colonize a little faster because when you shake them, you almost hit all sides of the grain with the LI. I always find the thicker solutions do the best. I just use karo in my pasty plates and noticed they leap off much faster than other normal agar plates ive used.




Maybe I'll try a bit more karo. Its still just nothing for the first 3 full days. In the past I've read people barely blending and having faster leapoff, but man, having all of that agar blended to make a thick broth makes it really really good on day 5.

I mean I'm happy with these 7-8 day colonization of a huge grain bag.... just wanna make sure I can't improve it even more.

When I get a chance I'll make a thick LC and compare. I'm just so lovin these LI for all the LI reasons.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26464018 - 02/01/20 04:53 PM (4 years, 16 days ago)

LI 4 LIFE! :rockon:

I do love slurries but i dont need 200 quarts in 4 days. LI can be tailor made for any size and volume. Tis why i like it so much. I used to simply blend up and suck into syringes. Then inject one syringe per 2-4 pf jars. A few days later slurry those into quarts. But i seemed to always have the same problem. Too much spawn too fast. Its a good problem to have.


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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26464307 - 02/01/20 08:16 PM (4 years, 16 days ago)



Okay, I don't know what I'm doing... but I made two 100cc LI syringes and I'm going to see what happens if I let them rest for a few days. Maybe the agar will jellify... maybe the broth will grow mycelia LC styles. Just an experiment.

Pasty made a ezLC tek similar... he says 7-10 days.

"You will soon notice that the nutes from the agar puck leach into the water (as will the food coloring if you use it) but any sediment or solid matter remain trapped in the agar puck. Give it some time to colonize, usually will take a week to 10 days. Swirling it aggressively will help expand the colony faster. You might dislodge the agar but that is not an issue."


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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