Home | Community | Message Board

Original Seeds Store
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag

Jump to first unread post Pages: 1 | 2 | 3 | 4 | Next >  [ show all ]
Offlinepsycho_nauticus
Stranger
 User Gallery

Registered: 01/26/20
Posts: 779
Loc: Incognito on Cloud 9
Last seen: 7 months, 24 days
SAB misting?
    #26455740 - 01/27/20 06:27 PM (4 years, 20 days ago)

Do you have to mist all the walls with water of your SAB inside before inoculating? It seems like you could accidentally ruin your jars and everything if it happens to get on it. I was planning on cleaning the inside and outside with 70% isopropyl alcohol. Do you guys mist the walls with water after that? Is that a necessity?


--------------------


Extras: Filter Print Post Top
OfflineNorthman
Stranger
Registered: 01/27/20
Posts: 61
Last seen: 3 years, 1 month
Re: SAB misting? [Re: psycho_nauticus]
    #26455764 - 01/27/20 06:41 PM (4 years, 20 days ago)

I mist everything with 5% bleach water. Mist the walls and the air really well. The point is for the water mist to drop bringing down anything airborne with it.


Extras: Filter Print Post Top
OfflinePrietenul
Friend

Registered: 11/22/19
Posts: 379
Loc: Germination
Last seen: 3 years, 5 months
Re: SAB misting? [Re: Northman]
    #26455797 - 01/27/20 07:04 PM (4 years, 20 days ago)

I usually mist before putting stuff in, then wipe the surface on which I'm working with Iso, wipe my jars and stuff and put them in.

Sometimes stuff is already inside the SAB (e.g. Agar bottle cooling or fresh poured petris), so I put an Iso soaked paper towel over them and then mist the SAB.

I just think it's not so good to mist your airbornes onto your jars and dishes... but someone else might have a different opinion.


Extras: Filter Print Post Top
OfflineNorthman
Stranger
Registered: 01/27/20
Posts: 61
Last seen: 3 years, 1 month
Re: SAB misting? [Re: Prietenul]
    #26455818 - 01/27/20 07:15 PM (4 years, 20 days ago)

I should have gone into further detail, apologies. I will typically already have my stuff in there, however I ALWAYS wipe everything down with iso again after misting and again before using whatever it is I may be using.

For example when doing agar transfers, my petris and scalpel and new petris will all already be in there. I mist and let the air settle. I then will wipe down the colonized petri and the new petri with iso soaked paper towels. I then wipe my scalpel and flame sterilize. I will then proceed to do my transfer. When finished I wipe everything again before moving to the next transfer.

It might be overkill but I have prefer to be cautious and not waste.


Extras: Filter Print Post Top
OfflineLogicaL ChaosM
Ascension Energy & Alien UFOs
Male User Gallery


Folding@home Statistics
Registered: 05/12/07
Posts: 69,599
Loc: The Inexpressible... Flag
Last seen: 2 hours, 18 minutes
Trusted Cultivator
Re: SAB misting? [Re: psycho_nauticus]
    #26455822 - 01/27/20 07:17 PM (4 years, 20 days ago)

I mist with water first than with iso propol. There is a draw back, water drippin from the ceiling of the SAB. That's an issue. Not sure what to do with that....


Extras: Filter Print Post Top
OfflinePrietenul
Friend

Registered: 11/22/19
Posts: 379
Loc: Germination
Last seen: 3 years, 5 months
Re: SAB misting? [Re: Northman]
    #26455835 - 01/27/20 07:22 PM (4 years, 20 days ago)

Quote:

Northman said:
I should have gone into further detail, apologies. I will typically already have my stuff in there, however I ALWAYS wipe everything down with iso again after misting and again before using whatever it is I may be using.

For example when doing agar transfers, my petris and scalpel and new petris will all already be in there. I mist and let the air settle. I then will wipe down the colonized petri and the new petri with iso soaked paper towels. I then wipe my scalpel and flame sterilize. I will then proceed to do my transfer. When finished I wipe everything again before moving to the next transfer.

It might be overkill but I have prefer to be cautious and not waste.




As I'm relatively new to this, I might be wrong (and corrected fast for sure :wink: ) but if you have contaminants in the air and "wash" them onto your dishes, it could be that you don't catch them all by wiping again after the mist.

Also, e.g, you have your petris upside down (agar on top) lying in your SAB. If you mist then, the contams will fall into the slits between the lids and the bottom of your dishes. You can't wipe with Iso there...

So for me the risk of contaminating stuff would be to high. I'd always either cover my stuff with paper towels or put it in after misting. Just my 2ct


Extras: Filter Print Post Top
OfflinePrietenul
Friend

Registered: 11/22/19
Posts: 379
Loc: Germination
Last seen: 3 years, 5 months
Re: SAB misting? [Re: LogicaL Chaos] * 1
    #26455837 - 01/27/20 07:22 PM (4 years, 20 days ago)

Quote:

LogicaL Chaos said:
I mist with water first than with iso propol. There is a draw back, water drippin from the ceiling of the SAB. That's an issue. Not sure what to do with that....




Why not just wipe it away with an Iso soaked paper towel?

Edit: Misting with Iso makes a bomb... :lol:


Edited by Prietenul (01/27/20 07:24 PM)


Extras: Filter Print Post Top
OfflineLogicaL ChaosM
Ascension Energy & Alien UFOs
Male User Gallery


Folding@home Statistics
Registered: 05/12/07
Posts: 69,599
Loc: The Inexpressible... Flag
Last seen: 2 hours, 18 minutes
Trusted Cultivator
Re: SAB misting? [Re: Prietenul]
    #26455839 - 01/27/20 07:23 PM (4 years, 20 days ago)

Hmmm....why didn't I think of that :strokebeard:


Extras: Filter Print Post Top
OfflineNorthman
Stranger
Registered: 01/27/20
Posts: 61
Last seen: 3 years, 1 month
Re: SAB misting? [Re: LogicaL Chaos]
    #26455841 - 01/27/20 07:24 PM (4 years, 20 days ago)

I've never had water drop from the ceiling of the sab before. I mist enough to thoroughly saturate the air but not enough for water to collect at the top.


Extras: Filter Print Post Top
OfflinePrietenul
Friend

Registered: 11/22/19
Posts: 379
Loc: Germination
Last seen: 3 years, 5 months
Re: SAB misting? [Re: Northman]
    #26455846 - 01/27/20 07:25 PM (4 years, 20 days ago)

Quote:

Northman said:
I've never had water drop from the ceiling of the sab before. I mist enough to thoroughly saturate the air but not enough for water to collect at the top.




Hm, if I make two-three pumps, I have droplets on the ceiling


Extras: Filter Print Post Top
OfflineNorthman
Stranger
Registered: 01/27/20
Posts: 61
Last seen: 3 years, 1 month
Re: SAB misting? [Re: Northman]
    #26455849 - 01/27/20 07:27 PM (4 years, 20 days ago)

I keep Pettis right side up in the sab and they are wrapped with parafilm/syran until I am ready to use them. I simply wipe them down, remove the film, wipe again and get to work.

This is just what works for me. Ideally I'd rather be working with a flow hood and not stress the sab. But in my many years of working with agar in a sab when I haven't had access to a flow hood, I've rarely had any contams.


Extras: Filter Print Post Top
OnlineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 2 minutes, 32 seconds
Trusted Cultivator
Re: SAB misting? [Re: Northman] * 1
    #26455853 - 01/27/20 07:30 PM (4 years, 20 days ago)

I never noticed water on the ceiling until one time I did a transfer and the second I put the lid back on a big drop landed square on the middle.

I spray air cleaner in first just cuz I have it then wipe ceiling and walls with Lysol wipes or iso towel.


--------------------
LAGM2020


Extras: Filter Print Post Top
InvisibleSir Pentinite
Stranger all the time.

Registered: 05/15/19
Posts: 525
Loc: ation Location Location
Re: SAB misting? [Re: A.k.a]
    #26455863 - 01/27/20 07:36 PM (4 years, 20 days ago)

I don't bother spraying anything.


--------------------
"I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'"
- Terrence McKenna


Extras: Filter Print Post Top
OnlineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 2 minutes, 32 seconds
Trusted Cultivator
Re: SAB misting? [Re: Sir Pentinite]
    #26455874 - 01/27/20 07:40 PM (4 years, 20 days ago)

You been doing it a while?

I started out wiping and spraying everything but as time goes on I’m doing less and less.

Then you get to the point where you’re running dry SAB and not wearing gloves lol.


--------------------
LAGM2020


Extras: Filter Print Post Top
OfflinePrietenul
Friend

Registered: 11/22/19
Posts: 379
Loc: Germination
Last seen: 3 years, 5 months
Re: SAB misting? [Re: Northman]
    #26455875 - 01/27/20 07:40 PM (4 years, 20 days ago)

Quote:

Northman said:
I keep Pettis right side up in the sab and they are wrapped with parafilm/syran until I am ready to use them. I simply wipe them down, remove the film, wipe again and get to work.

This is just what works for me. Ideally I'd rather be working with a flow hood and not stress the sab. But in my many years of working with agar in a sab when I haven't had access to a flow hood, I've rarely had any contams.




As I have occasional condensation on the lids, I always keep them with the agar on top. I guess this is how I got used to it. Write on the back, keep them upside down, put them in after misting.

Of course, if it works for you, it works. That's the most important thing. :wink:


Extras: Filter Print Post Top
OfflinePrietenul
Friend

Registered: 11/22/19
Posts: 379
Loc: Germination
Last seen: 3 years, 5 months
Re: SAB misting? [Re: A.k.a]
    #26455877 - 01/27/20 07:42 PM (4 years, 20 days ago)

Quote:

A.k.a said:
You been doing it a while?

I started out wiping and spraying everything but as time goes on I’m doing less and less.

Then you get to the point where you’re running dry SAB and not wearing gloves lol.




Yeah, until you hit contamination. Then you take care more again, until it lessens and lessens again. :lol: Live is mostly a (sine-)wave.


Extras: Filter Print Post Top
OfflineSockadin
Male User Gallery


Registered: 01/03/10
Posts: 7,244
Last seen: 3 months, 8 days
Trusted Cultivator
Re: SAB misting? [Re: Prietenul]
    #26455890 - 01/27/20 07:48 PM (4 years, 20 days ago)

I will Lysol the air in the SAB, but let be honest those things only work half the time. You put your hands in. You pull them out. I mean a still Air box is a lot of work.


Extras: Filter Print Post Top
OfflinePrietenul
Friend

Registered: 11/22/19
Posts: 379
Loc: Germination
Last seen: 3 years, 5 months
Re: SAB misting? [Re: Sockadin]
    #26455894 - 01/27/20 07:52 PM (4 years, 20 days ago)

Quote:

Sockadin said:
I will Lysol the air in the SAB, but let be honest those things only work half the time. You put your hands in. You pull them out. I mean a still Air box is a lot of work.




Isn't that dangerous, especially if you keep your burner close to the SAB?

For working with spores you'd still need a SAB, right?


Extras: Filter Print Post Top
Offlinepsycho_nauticus
Stranger
 User Gallery

Registered: 01/26/20
Posts: 779
Loc: Incognito on Cloud 9
Last seen: 7 months, 24 days
Re: SAB misting? [Re: Prietenul]
    #26455896 - 01/27/20 07:53 PM (4 years, 20 days ago)

I was thinking of misting before I put everything in, I just didn't know if it would be a problem opening the SAB back up after misting with water to put my stuff in? I have some jars and a syringe, no agar.

I'm going to wipe down with iso, but then mist with water. I feel like misting inside with everything already inside could be a problem because there is a higher risk of something getting on my items correct? With it being my first time doing bulk and a SAB I don't want to make the mistake of something misting or dropping on my stuff.

So opening the SAB back up after misting is okay then?


--------------------


Extras: Filter Print Post Top
Offlinevc77
incarnate
 User Gallery

Registered: 06/27/04
Posts: 1,302
Loc: PNW US
Last seen: 3 years, 10 months
Re: SAB misting? [Re: Sockadin] * 2
    #26455899 - 01/27/20 07:55 PM (4 years, 20 days ago)

Why mist when you don’t have to? Trust in gravity, your SAB works for a reason. I don’t wipe down my SAB, I don’t mist anything, I clean my SAB maybe once a month, maybe not even. It’s as easy as wiping down whatever you load into your dry-ass SAB and wiping your gloves down here and there as you work. Focus on refining your technique, hand efficiency and finesse.


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 | 4 | Next >  [ show all ]

Shop: Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* Indented Caps?
( 1 2 all )
MasFina 4,661 21 06/29/06 08:52 PM
by liamtheloser
* vicks cool mist humidifier MycelusWallace 1,955 12 02/03/03 07:59 AM
by MycelusWallace
* The BIG mist (automated)
( 1 2 all )
agar 2,242 24 01/30/06 12:42 PM
by tiny_rabid_birds
* grain to grain transfer - how do YOU do it ohmatic 4,024 12 05/16/06 08:48 AM
by mycijor
* cakes being indented while in incubation?? mexicosgreatest 702 6 05/25/08 06:00 PM
by RogerRabbit
* help! i'm misting with peroxide! shirley knott 6,680 9 04/09/03 05:42 PM
by Pantherina
* fruiting chamber @ ~93% rh - transfer casings to pmp for pinning? ohmatic 1,548 9 11/24/04 10:17 AM
by ohmatic
* Transfered cakes into new terrarium, got some questions. Smartweed 1,385 5 10/08/05 07:46 AM
by ShroomArtist84

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
758 topic views. 35 members, 164 guests and 33 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.008 seconds on 14 queries.