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OfflineRusty0
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Spore print, LC or Syringes?
    #26454641 - 01/27/20 07:03 AM (4 years, 21 days ago)

Alright lads, doing research in preperation for my first grow and for the longest time thought that it would be best if I started with a spore syringe or two and went straight into inoculation after prepping grain but after some more reading seems like people don't recommend this.

Initially I decided against starting on spore prints because of the extra steps involved in getting a good culture going on plates but I guess it's a useful skill to have that I'll have to learn after my first flush anyway.

I've not heard anything good about starting with a liquid culture. Why would you start with a LC if it's so bad? Or is it that it's just not very beginner friendly?


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Offlinetrubblesome
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Re: Spore print, LC or Syringes? [Re: Rusty0]
    #26454700 - 01/27/20 07:43 AM (4 years, 21 days ago)

Not beginner friendly. It's also something you have to make yourself if you live somewhere where live cubensis mycelium is illegal to possess or sell. You'll have to start with agar to make an LC anyway. Streaking a print to agar is not difficult and well, here, I'll just link you to this post I made the other day about how much time and worry you'll save by just starting out with the goal of creating a clean culture from spores so you can put it to grain and spawn to bulk without fretting every step of the way like I did my first time:

https://www.shroomery.org/forums/showflat.php/Number/26453311#26453311


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OfflineOdo
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Re: Spore print, LC or Syringes? [Re: trubblesome]
    #26454717 - 01/27/20 08:03 AM (4 years, 21 days ago)

LC isn't recommended because you can't always tell by looking at it that it is contaminated. I learned the hard way. I did eventually get LC to work but afraid to use it going forward. I made my LC from a dried cubensis that I found growing. I just used sterile tweezers to grab a tiny piece of gill and put it in the liquid.


Edited by Odo (01/27/20 08:08 AM)


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Offlinecarnalrekt
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Re: Spore print, LC or Syringes? [Re: Rusty0]
    #26454744 - 01/27/20 08:22 AM (4 years, 21 days ago)

If you can buy an LC legally its the easiest and  almost foolproof way to go. You can easily make more LC from it too without too much effort.Frequently gourmet cultures are purchased this way.

If your starting from print , you could probably make a dozen or more syringes from it, so its far more economical imho and its a good learning experience also.From there on the worlds your oyster mushroom..you could go pf-tek or agar or grains..Dont try making an LC from scratch until you have a clean culture on agar and you have your sterile technique down

Look up violets grain petri tek. Its a great easy way to obtain a clean culture
good luck!


Edited by carnalrekt (01/27/20 08:28 AM)


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OfflineMushkingMulah360
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Re: Spore print, LC or Syringes? [Re: carnalrekt]
    #26454783 - 01/27/20 08:56 AM (4 years, 21 days ago)

There’s also plenty of people I see going Ms to grain without a hitch. I think the biggest factor is getting good spores from a trusted person/vendor and the using the proper sterilization/techniques when inoculating. Myself being one of them. I will say now that I’ve started And have a bunch of jars and bags colonizing I got some plates going a few days ago and will be starting A2G from now on and working on some Lc in a few days too. So Ms to grain can def work fine just a little more risk. But I’m still a Noob myself, god luck brotha!


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Offlinetrubblesome
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Re: Spore print, LC or Syringes? [Re: MushkingMulah360]
    #26454805 - 01/27/20 09:12 AM (4 years, 21 days ago)

i keep seeing this strain of thought that people say it can't work - that's not the objection - it's just that 9 times out of 10 when someone posts a green tub or moldy jar asking "what went wrong? I did everything sterile!" it eventually comes out that they put a spore syringe direct to grain. I hate the feeling of finding mold in your jar or tub, and it's not much more effort/time to just start clean and prevent yourself from wasting weeks or months.

and I should add here - I was one of those people when I started out. spores direct to LC. got trich, was lucky to get a flush at all. don't be like me.


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OfflineMushkingMulah360
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Re: Spore print, LC or Syringes? [Re: trubblesome]
    #26454819 - 01/27/20 09:20 AM (4 years, 21 days ago)

Yea I hear ya, I keep seeing people saying that you can’t do Ms to grain. You can in fact successfully do it, I just made sure I was as clean and kept everything as sterile as I possibly could and do did my research and knew the proper techniques and they should be just fine. Like you said it’s just a higher risk for contams and things to go wrong potentially!


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Offlinetrubblesome
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Re: Spore print, LC or Syringes? [Re: MushkingMulah360]
    #26454836 - 01/27/20 09:30 AM (4 years, 21 days ago)

I don't see anyone saying that. I only see people saying you shouldn't, for all the reasons already stated.


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OfflineMushkingMulah360
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Re: Spore print, LC or Syringes? [Re: trubblesome]
    #26454863 - 01/27/20 09:51 AM (4 years, 21 days ago)

I guess it’s more just new people thinking they can’t do it.


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OfflineA.k.aM
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Re: Spore print, LC or Syringes? [Re: trubblesome]
    #26454865 - 01/27/20 09:51 AM (4 years, 21 days ago)

If you’re planning on doing this long term then go ahead and get a SAB and agar.

If you’re just trying to knock out some shrooms once in awhile syringe to wbs is fine.

I’ve done many syringe to grain grows with four different syringes and didn’t lose a jar. They weren’t all perfect but they all got the job done.

The pics in my signature are all syringe to wbs.


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LAGM2020


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OfflineMushkingMulah360
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Re: Spore print, LC or Syringes? [Re: A.k.a]
    #26454867 - 01/27/20 09:52 AM (4 years, 21 days ago)

^^^^ Fakts


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InvisibleJohnny.rotten
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Re: Spore print, LC or Syringes? [Re: A.k.a]
    #26455511 - 01/27/20 04:10 PM (4 years, 21 days ago)

Quote:

A.k.a said:
If you’re planning on doing this long term then go ahead and get a SAB and agar.

If you’re just trying to knock out some shrooms once in awhile syringe to wbs is fine.

I’ve done many syringe to grain grows with four different syringes and didn’t lose a jar. They weren’t all perfect but they all got the job done.

The pics in my signature are all syringe to wbs.




I Agree, why not do all 3. Put spores to grains, spores to agar, and spores to  LC. The agar only requires a drop, same with the LC. It's a learning process and why not try all of the methods.


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Offlinetrubblesome
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Re: Spore print, LC or Syringes? [Re: Johnny.rotten]
    #26455543 - 01/27/20 04:39 PM (4 years, 21 days ago)

100% - go for it to get fruits/see what the process is like, but have a back up in place. mushking I saw you put some stuff to agar too, hell yeah buddy.

I'm pretty sure mush cult is not a cult its just that everyone wants everyone else to succeed at this.


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InvisibleJohnny.rotten
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Re: Spore print, LC or Syringes? [Re: trubblesome]
    #26455570 - 01/27/20 04:59 PM (4 years, 21 days ago)

Yep, I put spore solution to grains for my first grow, but i also put some spores to no pour plates. Even though the spores to grains jars gave me fruits, it gave me the experience to see just how much better my grows got with agar.


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InvisibleGoombas69
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Registered: 12/15/19
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Re: Spore print, LC or Syringes? [Re: trubblesome]
    #26455584 - 01/27/20 05:06 PM (4 years, 21 days ago)

Actually you can go from MS syringe directly to liquid culture without the agar. I do both techniques and primarily use the agar to LC, but I keep the direct spore to LC for backup. Either way, I like to get mycelium from spore before I colonize to speed up colonization and avoid contamination. Always good to test your LC on agar when it's done since contamination isn't always discernible from looking at the LC.


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InvisibleJohnny.rotten
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Re: Spore print, LC or Syringes? [Re: Goombas69]
    #26455606 - 01/27/20 05:16 PM (4 years, 21 days ago)

Quote:

Goombas69 said:
Actually you can go from MS syringe directly to liquid culture without the agar. I do both techniques and primarily use the agar to LC, but I keep the direct spore to LC for backup. Either way, I like to get mycelium from spore before I colonize to speed up colonization and avoid contamination. Always good to test your LC on agar when it's done since contamination isn't always discernible from looking at the LC.



As i said, you can do all 3 to satisfy your curiosity. 1 spore syringe will go a long way. I got 2 syringes years ago, and they're both still 3/4 full, and still viable. Only a drop from the spore syringe is needed.


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Offlinespiritlands
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Re: Spore print, LC or Syringes? [Re: Johnny.rotten]
    #26455685 - 01/27/20 05:55 PM (4 years, 21 days ago)




Prints, lc and syringes are all skills you should have even if you don't use them all the time. I still use syringes from time to time but mostly work off print. Lc is awesome once you have good sterile technique.

First be able to make pitris that are clean consistently. This includes being able to isolate away from contams and make clean transfers. Then be able to generate clean spawn consistently. Then try making lc with CLEAN agar. After that you put them together and you have super powers.
Spores>agar>lc/li>grain.

I wouldn't start out with lc for your main project but I would at least learn it on the side. I was afraid of it for a long time bc ppl said it was too hard for a newb. Then I got better (stopped doing Ms>lc) and now it's easy. A magnetic stirrer makes a world of difference also.







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Germinating old spores progress page
how to calibrate your Martha



:spiralflip::spiralflip:




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OfflineA.k.aM
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Re: Spore print, LC or Syringes? [Re: spiritlands]
    #26455705 - 01/27/20 06:09 PM (4 years, 21 days ago)

With the mag stirrer does it grow faster or thicker or what?

I just made my first one and would swirl it or shake it a couple times a day, and it looks like it grew different from your picture.

Mine has more little pieces of myc that look kind of like bits of string, yours looks much more spread out.

I also started with an agar wedge the size of a bb.


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LAGM2020


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Offlinespiritlands
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Re: Spore print, LC or Syringes? [Re: A.k.a]
    #26455737 - 01/27/20 06:27 PM (4 years, 21 days ago)



↑that lc is pan cyan which is part of why it looks that way. The other reason is there are tons of colonies there but they're all settled on top of each other. Cube lc syringe↓


Idk that it makes it grow faster but I'm able to mix it really well and I assume reoxygenate the water while the vortex spins. I like it bc I don't have to shake and risk getting my gas exchange hole wet. Sometimes I leave it running all day other times I just use it to break up the myc.

These are my go-to lc jars when I start a new strain bc I'm able to hook leurlock syringes to the top and make multiple lc syringes without having to open the LC. Once I got confident making lc I use jars, bottles, whatever I can find.




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Germinating old spores progress page
how to calibrate your Martha



:spiralflip::spiralflip:




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OfflineA.k.aM
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Re: Spore print, LC or Syringes? [Re: spiritlands]
    #26455768 - 01/27/20 06:44 PM (4 years, 21 days ago)

Those are cool how it goes to the bottom, that’s perfect. I’ve been trying pouring it in the jars this time but when I made lc a while back I just had a silicone ship and had to tilt the jar to be able to suck it up into the needle

Worked but wasn’t very convenient.

Are those syringe filters?


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LAGM2020


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