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PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder

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OfflineDanito
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Which of these 3 plates looks cleanest for blender LI TEK?
    #26453730 - 01/26/20 03:09 PM (4 years, 3 days ago)

I NOCCED 10 bottles with an agar plate blended in sterile water the other day, and nothing happened! No contamination, but no growth of any kind was seen.

I think the mycelial growth on the plate was too weak, or maybe I incubated the bottles too cold?!?!

Anyway, I want to try again, so please help me decide which plate to blend up. Thanks for looking at them.

One


Two


Three


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OfflineA.k.aM
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Re: Which of these 3 plates looks cleanest for blender LI TEK? [Re: Danito]
    #26453772 - 01/26/20 03:33 PM (4 years, 3 days ago)

I think you need to give it more time.

When I swiped my lc to test it it did nothing until day 3-4 then came to life.


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Invisibleeatyualive
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Re: Which of these 3 plates looks cleanest for blender LI TEK? [Re: A.k.a]
    #26453818 - 01/26/20 04:10 PM (4 years, 3 days ago)

None. Id make transfers so you are not blending plates that have primorida on them.


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OfflineDanito
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Re: Which of these 3 plates looks cleanest for blender LI TEK? [Re: eatyualive]
    #26454046 - 01/26/20 06:52 PM (4 years, 3 days ago)

Thanks 'Eat'. And also many thanks all the TEKs you have shared. You have helped me a LOT.

First I learned how to do agar on Shroomery. Now I have decent plates, I have to confess that I have absolutely no clue what to do with them.... until I do some more research or ask a few more questions on Shroomery.

This place is heaven.


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Change your self, don't try to change the world


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Invisibleeatyualive
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Re: Which of these 3 plates looks cleanest for blender LI TEK? [Re: Danito]
    #26454067 - 01/26/20 07:10 PM (4 years, 3 days ago)

Do some transfers!


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OfflineDanito
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Re: Which of these 3 plates looks cleanest for blender LI TEK? [Re: A.k.a]
    #26454113 - 01/26/20 07:44 PM (4 years, 3 days ago)

You are right. Just when I was giving up hope (and had actually re-NOCCED several of them with a MS BRF slurry - dirty!) I started to see some fluffy white growth in some bottles this evening, and some of that way down the bottom. Looks like I was too impatient. Hahaha!

I did put them on perlite in an incubator though, with a heat controller and seedling mat.


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Change your self, don't try to change the world


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OfflineDanito
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Re: Which of these 3 plates looks cleanest for blender LI TEK? [Re: eatyualive]
    #26454122 - 01/26/20 07:52 PM (4 years, 3 days ago)

I did a bunch of transfers the other day. First time using an agar recipe containing LME. I think the malt failed to sterilise properly because there was bacteria inside the puck, and covering entire plates of the whole batch of 30 plates. LHBS sold me out-of-date malt extract - I am guessing that is the cause.

Will do some more transfers tomorrow.

Thanks again!


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Change your self, don't try to change the world


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OfflineSockadin
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Re: Which of these 3 plates looks cleanest for blender LI TEK? [Re: Danito]
    #26454127 - 01/26/20 07:54 PM (4 years, 3 days ago)

What I think eat is saying is that mycelium have a life cycle. Once they start to pin they become exhausted. If you are going to transfer do it before the pinning cycle.


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OfflineDanito
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Re: Which of these 3 plates looks cleanest for blender LI TEK? [Re: Sockadin]
    #26454147 - 01/26/20 08:05 PM (4 years, 3 days ago)

That is interesting and good to know. Thanks Sockadin.

I have a bunch of new agar plates ready - my SAB is finally comfortable to use - I am no longer scared of contamination, as I have seen lots :wink:

I will make the next few rounds as swiftly as possible.

I think I have overwhelmed myself with having too many clone cultures on the go. I just need to choose a few strong ones to work on and refrigerate or bin the rest I guess. I got greedy - newbie error I guess!


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PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder


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