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Offline920
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Registered: 01/04/20
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Shake the syringe ? I certainly did !
    #26453501 - 01/26/20 12:47 PM (4 years, 21 days ago)

Was preparing to inoculate some brf jars with a spore syringe today. I attached the needle and started shaking. Moments later I noticed spore water on the floor. When I looked at the syringe, maybe 1/2 cc was missing but also there was 3-4 cc's of air in the syringe and the plunger was fully raised. I know I didn't do that unless I shook it into that position.

I decided to put it aside and finish up a previously used syringe that had about 3 cc's remaining. Unfortunately I was only able to inoculate 3 of the 6 jars I set out to do.

Is that first syringe ruined -- i.e. probably contaminated?


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OfflineA.k.aM
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Registered: 10/27/19
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Re: Shake the syringe ? I certainly did ! [Re: 920]
    #26453509 - 01/26/20 12:57 PM (4 years, 21 days ago)

Did you push the air out??

Nothing you can do now, maybe you’ll get lucky and all the air was clean from swinging the syringe around making currents.

Agar or brf to find out now.


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LAGM2020


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InvisibleGoombas69
Amateur Mycologist

Registered: 12/15/19
Posts: 15
Re: Shake the syringe ? I certainly did ! [Re: 920]
    #26453514 - 01/26/20 01:00 PM (4 years, 21 days ago)

Safest thing to do is put some on an agar petri dish in a sterile environment and grow it out. It's actually more advantageous to work that way anyway because you inoculate with mycelium instead of spores, which shortens your colonization time by about a week and helps avoid contamination.


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OfflineSockadin
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Re: Shake the syringe ? I certainly did ! [Re: Goombas69]
    #26453525 - 01/26/20 01:10 PM (4 years, 21 days ago)

I doubt it is more contaminated than it was before you shook it. All fruiting happens in open air, so from fruiting to printing to spore syringe making (even from all of the sponsors) your syringe is 99.99% chance it has a contaminate in it.

  If you are going high yield bulk, work with it on agar in an SAB. IF your doing cakes in BRF jars. Just use it. You will probably never find out how contaminated it is unless you put it to agar.


Edited by Sockadin (01/26/20 01:10 PM)


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