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ShroomShroom1
Stranger
Registered: 09/25/19
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Why does selecting a faster myc on agar work better?
#26452912 - 01/26/20 03:51 AM (4 years, 3 days ago) |
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I know this may appear as a stupid question, but here I go.
I'm starting on agar, and read a few stuffs. People seem to always inoculate agar, and then select the fastest myc, cut it, and inoculate a new petri dish with it.
I understand that it makes for a faster growing myc, and so a faster colonization. That's what I found while searching.
But I felt like people were also saying that it makes for better shrooms, and I don't see why. Why would a selected myc makes a better harvest than a syringe, spores, or just a random petri dish?
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magicschoolbus



Registered: 02/19/18
Posts: 160
Loc: Shroomery
Last seen: 8 months, 15 days
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Re: Why does selecting a faster myc on agar work better? [Re: ShroomShroom1]
#26452964 - 01/26/20 05:52 AM (4 years, 3 days ago) |
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Well taking a small bit of the best performing mycelium will isolate the resulting transfer culture to contain genetics you selected from the previous plate.. this narrows down the genetic pool found in the culture which is good, because that means there's less competition amongst strains in the culture, meaning faster colonization times
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
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Re: Why does selecting a faster myc on agar work better? [Re: magicschoolbus] 1
#26452975 - 01/26/20 06:07 AM (4 years, 3 days ago) |
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Basically what he said.
if starting with spores there are millions of genetic possibilities, because every 2 spores that "fuck" create a set of unique genetics.
if you use a multispore syringe or sporeprint to agar, there will be choices. Choosing the fastest growing section of mycellium is always recommended.
If you keep selecting different genetics eventually you will have petri dishes that have perfect outward circular growth, where it all grows evenly in a 3d sphere.
Eventually when you get to this point you will have a single set of genetics.
There is no way to tell how theses genetics will perform without growing them out.
WHen you find a set that gives good yield + potency I call that a winner, and should be spreserved for future use, either on slants or just agar dishes.
I am drunk right now, but I know my shit. And when people say isolating the faster growing is better, they are for the most part leading you in the right direction.
Keep taking transfers to new dishes, find single sets of genetics and grow them out. Test, Test, Test.....
Sometimes the faster ones are not as potent.....it all depends.
P.S. For those of you who are wondering, yea, I think I'm back.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (01/26/20 06:08 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Why does selecting a faster myc on agar work better? [Re: PussyFart]
#26453031 - 01/26/20 07:01 AM (4 years, 3 days ago) |
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Sockadin



Registered: 01/03/10
Posts: 7,244
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Re: Why does selecting a faster myc on agar work better? [Re: PussyFart]
#26453084 - 01/26/20 07:47 AM (4 years, 3 days ago) |
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Quote:
PussyFart said:
I am drunk right now, but I know my shit. Test, Test, Test.... P.S. For those of you who are wondering, yea, I think I'm back.
This is all I read!!!! Welcome back Pussyfart!
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ShroomShroom1
Stranger
Registered: 09/25/19
Posts: 43
Last seen: 2 years, 11 months
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Re: Why does selecting a faster myc on agar work better? [Re: PussyFart]
#26453168 - 01/26/20 08:58 AM (4 years, 3 days ago) |
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Quote:
PussyFart said: Basically what he said.
if starting with spores there are millions of genetic possibilities, because every 2 spores that "fuck" create a set of unique genetics.
if you use a multispore syringe or sporeprint to agar, there will be choices. Choosing the fastest growing section of mycellium is always recommended.
If you keep selecting different genetics eventually you will have petri dishes that have perfect outward circular growth, where it all grows evenly in a 3d sphere.
Eventually when you get to this point you will have a single set of genetics.
There is no way to tell how theses genetics will perform without growing them out.
WHen you find a set that gives good yield + potency I call that a winner, and should be spreserved for future use, either on slants or just agar dishes.
I am drunk right now, but I know my shit. And when people say isolating the faster growing is better, they are for the most part leading you in the right direction.
Keep taking transfers to new dishes, find single sets of genetics and grow them out. Test, Test, Test.....
Sometimes the faster ones are not as potent.....it all depends.
P.S. For those of you who are wondering, yea, I think I'm back.
Thanks for that reply.
So I guess the only thing that matters are the isolation of 1 strain, hoping it's a good one so it can be grow again and again. The fact that it was the fastest grower is just a technical consequence, although it doesn't do any harm, right?
And transfers are done until it grows in a circle shape, meaning we isolated only 1 strain, right?
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Hans Wermhat
Human


Registered: 06/10/19
Posts: 167
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Re: Why does selecting a faster myc on agar work better? [Re: ShroomShroom1]
#26453339 - 01/26/20 11:12 AM (4 years, 3 days ago) |
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Right, the fastest growing sectors won't necessarily be the highest yielding or the most potent, but speed is really the only thing we can gauge on agar alone. Yield and potency have to be tested by actually growing out the isolate. Because a single germination plate can have thousands of strains, it's impractical/impossible to test them all for yield&potency, so we narrow it down by selecting only the fastest sectors and hope that at least one of these speedy isolates will be winners in the other areas (yield&potency) as well.
-------------------- Thank you for taking the time to read this message. Have a wonderful day.
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Newbie
User of semicolons.



Registered: 07/18/04
Posts: 24,710
Loc: SoCal
Last seen: 1 day, 9 hours
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Re: Why does selecting a faster myc on agar work better? [Re: PussyFart]
#26453375 - 01/26/20 11:34 AM (4 years, 3 days ago) |
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Quote:
PussyFart said: I am drunk right now, but I know my shit.
If I had a nickel...
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Why does selecting a faster myc on agar work better? [Re: PussyFart]
#26453416 - 01/26/20 12:00 PM (4 years, 3 days ago) |
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Quote:
PussyFart said: Basically what he said.
if starting with spores there are millions of genetic possibilities, because every 2 spores that "fuck" create a set of unique genetics.
if you use a multispore syringe or sporeprint to agar, there will be choices. Choosing the fastest growing section of mycellium is always recommended.
If you keep selecting different genetics eventually you will have petri dishes that have perfect outward circular growth, where it all grows evenly in a 3d sphere.
Eventually when you get to this point you will have a single set of genetics.
There is no way to tell how theses genetics will perform without growing them out.
WHen you find a set that gives good yield + potency I call that a winner, and should be spreserved for future use, either on slants or just agar dishes.
I am drunk right now, but I know my shit. And when people say isolating the faster growing is better, they are for the most part leading you in the right direction.
Keep taking transfers to new dishes, find single sets of genetics and grow them out. Test, Test, Test.....
Sometimes the faster ones are not as potent.....it all depends.
P.S. For those of you who are wondering, yea, I think I'm back.
Wow how the hell ya been man? Good to see you.
-------------------- Trade List 🖕🖕🖕 6 hole Mini Monos
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