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OfflineBackbone
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How many times can you clone before senescence becomes an issue?
    #26451915 - 01/25/20 01:42 PM (4 years, 4 days ago)

and what do you do once it does do you use a regular ms from your own culture and make transfers on agar until you get a good strain? or what sorry ignorance. Or when you clone is each section of a petri dish a different strain therefore you reserve you petri dish for future grows?


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OfflinecronicrFacebook
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Re: How many times can you clone before senescence becomes an issue? [Re: Backbone]
    #26452012 - 01/25/20 02:32 PM (4 years, 4 days ago)

once u make a clone store the culture, don't keep cloning clones.
Some think sectoring of clones is separate strains but it isn't proven


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OfflineBackbone
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Re: How many times can you clone before senescence becomes an issue? [Re: cronicr]
    #26452030 - 01/25/20 02:43 PM (4 years, 4 days ago)

ill figure it out until someone corrects what im doing hopefully i just wasnt sure how many transfers you could make from agar and how many times you could do that before you used all of your mushroom i guess its not really important for my case jw


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Re: How many times can you clone before senescence becomes an issue? [Re: Backbone]
    #26452047 - 01/25/20 02:52 PM (4 years, 4 days ago)

transfers won't cause any issues, not much cell division happens on plates. as opposed to expanding on grain and g2g then bulk


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Re: How many times can you clone before senescence becomes an issue? [Re: cronicr]
    #26452053 - 01/25/20 02:56 PM (4 years, 4 days ago)

ah k something id probably learn but thanks for the info


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