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OfflineBackbone
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I have a bag of inoculated, store, shitake dowels can i make more dowels with the mycelium on these?
    #26450593 - 01/24/20 07:09 PM (4 years, 5 days ago)

can i?


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Offlineiwh678
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Re: I have a bag of inoculated, store, shitake dowels can i make more dowels with the mycelium on these? [Re: Backbone]
    #26450672 - 01/24/20 07:44 PM (4 years, 5 days ago)

You can try. I've had bad luck trying to get a clean culture from dowels, but then again I only tried from on batch.

If you have 100 then you have plenty to inoculate a few logs. Save a few to try to culture onto agar and use the rest on logs. I would say that's your best bet. Once the logs fruit you can cone them and move that culture to more dowels.


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OfflineBackbone
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Re: I have a bag of inoculated, store, shitake dowels can i make more dowels with the mycelium on these? [Re: iwh678]
    #26450738 - 01/24/20 08:18 PM (4 years, 5 days ago)

ah see im still confused about cloning and agar from what i understand mycelium from a few grain jars eventually stops (?) or "degenerates"...not sure if thats what was implied in what i read on here in a thread. Is the point to grow the mushrooms and then use the spores...? hah what is cloning...sorry you stumbled into a major noob i only have experience with pf tek and spore prints


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Offlineiwh678
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Re: I have a bag of inoculated, store, shitake dowels can i make more dowels with the mycelium on these? [Re: Backbone]
    #26451170 - 01/25/20 04:25 AM (4 years, 4 days ago)



Cloning is taking a piece of tissue from from a mushroom, petri dish, ect and moving it to culture (usually on a petri dish). You will get an exact genetic copy of it. A clone.

You can transfer enough that you cause senescence. Basically the culture gets so used to the media it "forgets" how to grow on anything else. There are a few ways around this.
1)From your original culture, your "Generation 1", you create what is called a master slant. It is a long term storage culture that you pull from when needed.
2)Grow from spores

Making hardwood logs is honestly the easiest way to grow mushrooms in my opinion. It just takes patience. You will drill holes in a log (make sure its hardwood)in a diamond patter like this.

These are probably a little close together. I do mine ~4-5 inches apart. Then you hammer in your dowels and dab a little bit of wax to cover it.


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OfflineBackbone
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Re: I have a bag of inoculated, store, shitake dowels can i make more dowels with the mycelium on these? [Re: iwh678]
    #26451607 - 01/25/20 10:23 AM (4 years, 4 days ago)

i got it now thanks that diagram wouldve came in handy dont know why i didnt look for something like that instead of read a books worth of material


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Offlinetrubblesome
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Re: I have a bag of inoculated, store, shitake dowels can i make more dowels with the mycelium on these? [Re: Backbone]
    #26451615 - 01/25/20 10:30 AM (4 years, 4 days ago)

heres a walk through on prepping and inoculating wood dowels:

https://blog.freshcapmushrooms.com/learn/mushroom-plugs-log-cultivation/

instead of “grain spawn, wedges of mycelium on agar, or even liquid culture syringes.” like it says there, youll inoculate the new dowel plugs with a few of the dowel plugs you already have.


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Offlineiwh678
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Re: I have a bag of inoculated, store, shitake dowels can i make more dowels with the mycelium on these? [Re: Backbone]
    #26451628 - 01/25/20 10:43 AM (4 years, 4 days ago)

Quote:

Backbone said:
i got it now thanks that diagram wouldve came in handy dont know why i didnt look for something like that instead of read a books worth of material




That diagram is from a book. Its called "Growing Gourmet and Medicinal Mushrooms" by Paul Stamets. I would recommend buying it


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