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Offlinezaatar
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Milky colonization on agar
    #26450387 - 01/24/20 05:21 PM (4 years, 23 days ago)

Hello friends,

I searched the forums and saw several threads pointing out that agar dishes with milky growth (especially when using syringe to agar) are often contamination. But! Most of these threads were from years ago and the pictures were down.

I'd like to know whether the following plate looks contaminated:

Note: this has been incubating for four days at about 78 degrees F


Agar recipe used:
10g Extra Light malt Extract
10g agar
1g nutritional yeast

Inoculation method:
Syringe to agar in a SAB

Likely issues:
- I did not see many black flakes at all in the syringe so I ended up using about 0.25 cc per petri dish, hoping to get a good number of spores on each plate. I may have overdone it.
- The agar was poured warm and caused a good amount of condensation. When I let the agar cool, it clumped together too much. So I heated it up again but likely poured when it was still too hot.
- I may have inoculated the dishes when the agar was still warm. It had gelled, but there could have been some heat still coming off.

Going forward:
- I would like to try pastywhyte's no pour agar TEK and inoculate from spore prints.
- I would like to know what happened to these dishes and where the mistake was

Thank you for any input!


Edited by zaatar (01/24/20 05:27 PM)


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Re: Milky colonization on agar [Re: zaatar]
    #26450401 - 01/24/20 05:34 PM (4 years, 23 days ago)

That's bacteria. Most likely from the syringe.


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Offlinezaatar
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Re: Milky colonization on agar [Re: mushboy]
    #26450405 - 01/24/20 05:37 PM (4 years, 23 days ago)

Quote:

mushboy said:
That's bacteria. Most likely from the syringe.





Glorious! Thank you

Any use in growing this out to see if mycelium develops and to then take a transfer?

Or just toss it and try again from a spore print?


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Re: Milky colonization on agar [Re: zaatar]
    #26450407 - 01/24/20 05:37 PM (4 years, 23 days ago)

+1 for Bacteria

I would toss that dish...it's done


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Re: Milky colonization on agar [Re: zaatar]
    #26450443 - 01/24/20 06:02 PM (4 years, 23 days ago)

Quote:

zaatar said:
Quote:

mushboy said:
That's bacteria. Most likely from the syringe.





Glorious! Thank you

Any use in growing this out to see if mycelium develops and to then take a transfer?

Or just toss it and try again from a spore print?





You could,  but I'd just toss and try again.

Bacteria doesnt expand the way mold does. If the spores do germinate myc can outgrow the bacterial colonies but the bacteria can also get mashed in the myc and cause a shit show.



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Re: Milky colonization on agar [Re: mushboy]
    #26450497 - 01/24/20 06:22 PM (4 years, 23 days ago)

The dish will be tossed. Thanks for the feedback! I hope this will be helpful to other newbies out there trying to go syringe to agar


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Re: Milky colonization on agar [Re: zaatar]
    #26450500 - 01/24/20 06:24 PM (4 years, 23 days ago)

Only use a drop or two of solution :cheers:


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