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Offlinemr.nicholas
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Registered: 10/24/18
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any of this rhizomorphic?
    #26449616 - 01/24/20 09:08 AM (4 years, 5 days ago)

Still relatively new at this and wondering if any of this looks rhizomorphic/healthy and worth continuing? My last grow stalled/never fruited because I think I had tomentose mycelium that I grew from some old dry mushrooms, but these are all from spores. Anything here look worth pursuing? Just looking for a little advice.

Thank you!









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OfflineKmacmo
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Registered: 08/14/19
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Re: any of this rhizomorphic? [Re: mr.nicholas]
    #26449629 - 01/24/20 09:15 AM (4 years, 5 days ago)

Nothing wrong with tomentose mycelium but they all look contaminated I wouldn't open any of those in your place of work


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OfflineThe Mycologist
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Re: any of this rhizomorphic? [Re: mr.nicholas]
    #26449630 - 01/24/20 09:15 AM (4 years, 5 days ago)

Take transfers from the first one on its right side.


You arent going for rhizo. Just clean.


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OfflineSynKyd
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Re: any of this rhizomorphic? [Re: The Mycologist]
    #26449823 - 01/24/20 11:46 AM (4 years, 5 days ago)

Quote:

The Mycologist said:
Take transfers from the first one on its right side.


You arent going for rhizo. Just clean.




:whathesaid:

I would take several from right side and a couple from across the plate while I’m in there.


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OfflineA.k.aM
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Re: any of this rhizomorphic? [Re: SynKyd]
    #26449864 - 01/24/20 12:05 PM (4 years, 5 days ago)

Are you misting them or is that on a lid?

You can a lot of times make the same mycelium go rhizo or tomentose by adjusting your agar, all the same stuff.

Most look workable except the third one, that ones shot.


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Offlinemr.nicholas
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Re: any of this rhizomorphic? [Re: A.k.a]
    #26450636 - 01/24/20 07:28 PM (4 years, 5 days ago)

I was under the impression that rhizomorphic was much more likely to fruit than tomentose. I'll follow through, but also considering starting some more spores to see if I can get the rhizo.

Thanks for the tips!


Quote:

A.k.a said:
Are you misting them or is that on a lid?





I'm not misting them, the condensation forms after I move my petri dishes from storage into the incubator. It's pretty humid where I live... is it from something else?


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OfflineA.k.aM
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Re: any of this rhizomorphic? [Re: mr.nicholas]
    #26451126 - 01/25/20 03:08 AM (4 years, 4 days ago)

Lol no that makes sense, just wondering.

If you’re pouring your own plates make some with half the normal nutes and pour it a little thinner and take a transfer from the plate you have now. I’d bet it goes more rhizo.

I’ve only done a handful of germ plates but rhizo seems to be rare on them.


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OfflineKmacmo
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Re: any of this rhizomorphic? [Re: A.k.a]
    #26451208 - 01/25/20 05:22 AM (4 years, 4 days ago)

Yeah rhizo is very likely to fruit.
Like a.k.a said thinner plates less nutes should do it :smile:
Had a few germ plates with rhizo but it is kinda rare, usually get some on transfer 1 or 2 now.

When I swipe spores on I can always see atleast some spores on the plate where it was swiped, tried one plate with the most tinyest dab of spores and just pressed it into the center of the plate you couldn't see any spores with your eye... Let it grow out quite abit and it had multiple rhizo sectors more than I've ever had before on a single germ plate.

I think it's about getting the smallest amount of spores on the plate  so they don't all grow over each other causing the fluffy/tomentose growth, what do you guys think?

I don't use incubators just prefer room temp so they don't sweat and condense so much, it's slower but I'm not in a hurry.


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OfflineA.k.aM
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Re: any of this rhizomorphic? [Re: Kmacmo]
    #26451447 - 01/25/20 08:45 AM (4 years, 4 days ago)

Yeah I agree with the germ plate thing, def seems like there’s too many spores going off for one to establish and go rhizo.


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Offlinemr.nicholas
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Re: any of this rhizomorphic? [Re: A.k.a]
    #26451512 - 01/25/20 09:25 AM (4 years, 4 days ago)

Thanks guys. Less spores, less nutrients, and thinner pours on the next run.

Excuse my ignorance but germ plate = petri dish right?

I know there are many ways to do this... any other recommendations for getting good rhizomorphic growth early on?


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OfflineA.k.aM
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Re: any of this rhizomorphic? [Re: mr.nicholas]
    #26451734 - 01/25/20 11:51 AM (4 years, 4 days ago)

Yep germination plate/Petri.

The less nutes the more likely you’ll see rhizo. It really doesn’t matter much, I just had a plate half rhizo half fluffy and took a transfer from each and the rhizo is actually much slower which I never expected.

It does look cooler though.


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