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ComebackKid
Multispore Enthusiast



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CLONE WARS 4
#26447756 - 01/23/20 07:35 AM (4 years, 5 months ago) |
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CLONE WARSLet's grow some clones!-Post a pic of a fruit you are about to clone -Then edit your post with the results! -New clones preferred but if you have some real beauties dont be shy -Any and all things clones welcome in this thread Let's have some fun I'll try and link as many user's posts as I can in the OP: Clones: - CBK's Lucy side pin- YogiBear's Upside Downer PE6- A.k.a.'s First Timer- Zifozonke's 5 big boys with PE- P9hu7's cluster clone- MLPismyOPSEC's APE fruit + pin & B+ cluster clone!!!!!- Cronicr's Cronenberg Clone- Trubblesome's nip clone- vc77's nip clone- Spiritlands' late flush redboy - Staytrippy420's Dusty ass RWHall Of Fame Great pinners:Highest yields:Uniform growers:Mutants:
Edited by ComebackKid (01/27/20 02:06 PM)
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
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I found this cool lookin side pin while harvesting some ESS lastnight. Hoping to get some mutants or leucistic lookin fruits from it.
Took two tissue samples to agar. Praying I get some clean growth!
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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YogiBear



Registered: 08/24/19
Posts: 845
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I'm working on this UPSIDE DOWNER PE6 right now 😆
Doesn't look promising though as my first attempt at cloning.
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A.k.a
Stranger



Registered: 10/27/19
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Pretty good timing for me, nothing super interesting but my first run at clones.
Cloned the biggest one on the left side and the tallest one in that back cluster.

Spawned on the 8th.

Some early plates with a tissue plate. Didn’t take any of the actual plates I put to grain.

Currently waiting for jar to colonize with a clone of this fat one in the middle, which also popped up days before the others.

Here it is as of today, 1-24

I’m not sure what’s going on here. I’ve never had problems with stretching before so I’m leaning towards genetics, but I’ve also never run a clone or dealt with this dense of an area of pins.
1-25 Finished overnight, thought I had another day at least. Tiny guys.
   Was around 270 wet from the first shoebox.
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LAGM2020     
Edited by A.k.a (01/25/20 11:05 AM)
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: CLONE WARS [Re: A.k.a]
#26447941 - 01/23/20 10:20 AM (4 years, 5 months ago) |
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-------------------- Tek's I use LAGM2020
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
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Love it!
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Zifozonke
Stranger


Registered: 03/24/19
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Jumpin in on the action here..have never participated so thought why the hell not. Got clones going from two different varieties started end of last year~My first grow each of PE and RW
Ill start with the PE Cloned the two largest fruits from this shoebox(60g&80g)
 Went onto agar...
 (1&2)Each plate went into a myco quart of grain and were spawned 17/1 into a recycled ice cream container of coir 1:2(will add pics)
2/2/2020*update*

Update17/2*update* Pe clone 1....

Pe clone 2
 Got excited when pins started...but then...this...wetspot/bacterial/pseudomonas....have never had this before so was a bit bummed...coulda been from me over misting my casing-im not sure-but just left lid off and it got slightly better
 But I know it would have been better if this condition was absent.
 Off one myco quart spawn. Going back to my agar plates-to run these both again-I think potential is there.What I really liked in second clone was there was no blobbing at all.Gotta just get past these contams...
Oh well ....win some ...loose some....
Spawned 4/5 of my RWs One clone is a little behind Hopefully something good will come from them..fingers crossed
RW clones coming from this tub started from spores
 5 of the largest fruits were carefully selected
 Heres what the dishes look like
 Each transfer will again get its own quart of grain and recycled container to check side by side results...
2/2/2020*update* Jars are coming along nicely....almost ready for first shake.

Really looking foward to seeing and sharing results....
9/3 So heres my long over due update with my RW tubs. Ended up with mean green in two out of my five tubs like this...
 Damn I hate the stuff.... The others didnt turn out too bad -but not great either.Think my tubs were slightly bacterial-I dunno. Plates looked clean but ay results dont lie so...gotta just put my nise down and work harder. Anyway here the results RW Clone1-mean green
Clone2

Clone3-mean green
Clone4

Clone5

So there we have it. Clone 4 put out the most but not by much ...and clone 5 had the biggest fruits but was lacking on weight. I really thought I was gonna do much better but seems like I had some bacteria/mould issues piggy backing these two grows(PE&RW)
Going to work on my plates again & Im going to try some tubs with straw and hpoo this time round to see if things will be better...who knows
Edited by Zifozonke (03/09/20 12:42 PM)
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,904
Loc: Canada
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RW clone taken from cluster on the left > agar > oats:

Grain master:

More to follow.
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MLPismyOPSEC
That One Ponyfucker


Registered: 11/13/18
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These are all new clones in that they have not been grown out yet, and in fact these are my first clones ever! I spawned them to tubs just yesterday 
APE-FC (fruit clone); Cloned the tall bastard in the back right. He got a good amount bigger than that, this was a hella early picture of that SB.

Decent clone, put off 302 wet g/qt (~21.1 dry g/qt), i'll keep 'er around. Spawn to harvest: 27 days.

APE-AC (agar clone); Cloned that pin dead center in the picture.

Very meh clone, i was hoping for better! Put off 261 wet g/qt (~18.3 dry g/qt). Short but dense AF. Spawn to harvest: 20 days.

B+; This pretty lookin cluster.

Pretty happy with this one! Put off 427 wet g/qt (~29.9 dry g/qt). Definitely keeping this one. Spawn to harvest: 18 days.

All of them were run in 32qt unmodded monos, 3 qts spawn each, 10 qts CV (1:4 ratio), spawn could definitely have been cleaner, but i'm not too upset about the results. This was a total of a 9 mono + 6 shoebox run, with 10 more monos behind it, will be harvesting those starting over the weekend. I have been fucking BUSY.
Edited by MLPismyOPSEC (02/26/20 11:42 AM)
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Zachsonpub
Friend



Registered: 03/02/16
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Got some 2nd flushes coming up. If I like what I see, I'll join in.
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cronicr



Registered: 08/07/11
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Loc: Van Isle
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 I'm gonna clone this but not sure how I will proceed just yet being that it is 2 blobs with a few fruits growing on top of them lol
 picked this guy who weighed in at 102 grams wet
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cronicr



Registered: 08/07/11
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Re: CLONE WARS [Re: cronicr]
#26448685 - 01/23/20 05:43 PM (4 years, 5 months ago) |
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 Updated pic( wouldn't let me upload earlier)
-------------------- It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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ComebackKid
Multispore Enthusiast



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Re: CLONE WARS [Re: cronicr]
#26448710 - 01/23/20 05:51 PM (4 years, 5 months ago) |
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Is that the same cluster cron?
Just updated the OP welcome to the clone wars fam!!
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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A.k.a
Stranger



Registered: 10/27/19
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Only now with extra bugs.
I just know I’m gonna have problems with that shit in a couple months.
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LAGM2020     
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ComebackKid
Multispore Enthusiast



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Re: CLONE WARS [Re: YogiBear] 1
#26448732 - 01/23/20 06:01 PM (4 years, 5 months ago) |
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Quote:
YogiBear said: I'm working on this UPSIDE DOWNER PE6 right now 😆
Doesn't look promising though as my first attempt at cloning.

Yogi usually I'll rip my fruit in half from the bottom of the stipe right through to the tip of the cap Then I take either flesh from the inside of the cap head or tease a tiny bit of tissue from the stipe. Smaller the piece you can get on the end of the scalpel the better IME
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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cronicr



Registered: 08/07/11
Posts: 61,478
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Quote:
ComebackKid said: Is that the same cluster cron?
Just updated the OP welcome to the clone wars fam!!
Yepper this grow was doomed from the start Lol, sat for a month colonized then released a million gnats then blobbed out
-------------------- It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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A.k.a
Stranger



Registered: 10/27/19
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Quote:
ComebackKid said:
Quote:
YogiBear said: I'm working on this UPSIDE DOWNER PE6 right now 😆
Doesn't look promising though as my first attempt at cloning.

Yogi usually I'll rip my fruit in half from the bottom of the stipe right through to the tip of the cap Then I take either flesh from the inside of the cap head or tease a tiny bit of tissue from the stipe. Smaller the piece you can get on the end of the scalpel the better IME
Why is a smaller piece better? I have noticed it seems to start growing on the plate faster when it’s tiny. One of the last ones I did a piece of tissue the size of a grain of sand fell off the blade onto the plate and grew at least as quickly as the main chunk.
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LAGM2020     
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YogiBear



Registered: 08/24/19
Posts: 845
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Thank you CBK. I'm so enjoying this part of mush cult. Been avoiding it until I had my FH built.
LET THE GAMES BEGIN
I have a 3rd flush PE6 tray coming in to maturation so may grab one of them and do it right.
I will never win this but it's pushing me into areas that I need to be heading in
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ComebackKid
Multispore Enthusiast



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Re: CLONE WARS [Re: YogiBear] 1
#26448985 - 01/23/20 08:50 PM (4 years, 5 months ago) |
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Quote:
A.k.a said: Why is a smaller piece better? I have noticed it seems to start growing on the plate faster when it’s tiny. One of the last ones I did a piece of tissue the size of a grain of sand fell off the blade onto the plate and grew at least as quickly as the main chunk.
Smaller pieces have less chance of carrying bacteria along with it
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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trubblesome
Stranger



Registered: 11/09/19
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Last seen: 1 year, 3 months
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Cloned one of these nipply lookin shrooms last week.

made first transfer on my "nip B+" clone tonight

Update 4/15Okay, spawned Nip B+ and Big GT today. These are the deepest shoeboxes I've ever done. spawned 1:2.5 with a half quart casing layer. forgot to get pics of the jars pre-spawn.  I hit the jackpot on clusters with this PESA tub. Unfortunately I also hit the jackpot on mold. I caught it before it went green but I cut this whole section out - the casing wasn't colonizing and the tub began to get a mildew smell. I cut it out and put it in a bowl and on close examination, what appeared to have been knots was the beginning of some kind of mold growing. I put the bowl outside and the next day it was green, maybe trich Anyway I think I bought myself enough time to get a decent harvest as well as get a couple clones from these clusters. I think the clusters came from how I chose the mycelium from the plates but I'll leave that for another post. I will probably do something similar with the clones I gather from this tub... Update 4/24clone dishes looking good, clean as fuck, which is a relief since I had a bad SAB session last week and had to make a ton of transfers last night to get away from shit on dishes. I also realized I could just drop all these in to their own jars or in to one bag and mix the genetics at spawn or inoculation rather than waste time dicking around with enough plates to have every combo covered. so I'll probably do that. I'm gonna drop fat boy in its own grain jar at least.  The clone shoeboxes are about to pop. guessing I'll have a pinset going monday. hopefully the nip was genetic. I don't even remember what "Big GT" looked like, so hopefully it's just fucking big and has a nice cap for printing. Nip B+:  Big GT:
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Edited by trubblesome (04/24/20 11:41 AM)
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Thekd95
Not your buddy GUY

Registered: 06/15/18
Posts: 556
Last seen: 1 year, 1 month
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Nice nips
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YogiBear



Registered: 08/24/19
Posts: 845
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Quote:
trubblesome said: Cloned one of these nipply lookin shrooms last week.

made first transfer on my "nip B+" clone tonight

Couldn't resist 😆
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YogiBear



Registered: 08/24/19
Posts: 845
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Quote:
YogiBear said: I'm working on this UPSIDE DOWNER PE6 right now 😆
Doesn't look promising though as my first attempt at cloning.

Grabbed 2 pieces for a T1
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A.k.a
Stranger



Registered: 10/27/19
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Updated my first maz clone.
They seem to be stretching a little bit, I’ve never had an issue with that before. Genetics??

I did clone taller ones so maybe theyre going tall first then fill out later??
Lol I hope.
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LAGM2020     
Edited by A.k.a (01/24/20 09:48 AM)
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
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Re: CLONE WARS [Re: A.k.a]
#26449778 - 01/24/20 11:10 AM (4 years, 5 months ago) |
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Doesn't look like anything out of the ordinary happening there. I bet they all shoot up really soon and even out They look purdy tho
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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A.k.a
Stranger



Registered: 10/27/19
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Thanks man, it’d be cool if they all evened out. Aren’t clones supposed to grow more evenly that way, like all mature closer together than an ms grow? A lot of the pins definitely showed up simultaneously.
I’ve actually got three going, but the one I’ve been posting was the only one I was sure had clean spawn and pinned first.
I took two samples from each of the fruits I cloned, but had a labeling mix up when I took them way back so I only know for sure what one is. I’m guessing the two shoeboxes on the right are from the same mushroom.

The left one definitely likes the light, I flipped it around this morning to try and get them to go back straight up.
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LAGM2020     
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vc77
incarnate


Registered: 06/27/04
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Re: CLONE WARS [Re: A.k.a]
#26449962 - 01/24/20 12:51 PM (4 years, 5 months ago) |
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Ran a mono and four shoeboxes of BHT from an old print. Hunting that nipple/pointy-hat expression. A bunch of fruits had that borderline semi-nipple look but one decided to throw a couple fruits that were bang on nips. Let’s see how this clone runs
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trubblesome
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Re: CLONE WARS [Re: vc77]
#26450125 - 01/24/20 02:12 PM (4 years, 5 months ago) |
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hell yeah nip crew
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ComebackKid
Multispore Enthusiast



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I don't really clone often but I did have one really great PFC clone that had very uniform growth and the fruits matured pretty well all at once. Dense meaty stems. Killer potency. Amazing yields. Miss that clone. It was taken from a crippled side pin of all things. I was hoping to get mutants but it grew out beautifully.
>  Other than that I havent had much luck with clones TBH. The last clone I ran pinned at all different times and yielded lower than an average MS run would.
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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jcm4620
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Quote:
ComebackKid said: I don't really clone often but I did have one really great PFC clone that had very uniform growth and the fruits matured pretty well all at once. Dense meaty stems. Killer potency. Amazing yields. Miss that clone. It was taken from a crippled side pin of all things. I was hoping to get mutants but it grew out beautifully.
>  Other than that I havent had much luck with clones TBH. The last clone I ran pinned at all different times and yielded lower than an average MS run would.
lol holy shit that thing looks crazy as hell but ya those turned out great lookin. its kinda kinda like when 2 ugly ass ppl produce a smokin hot daughter.🤪
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spiritlands



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Re: CLONE WARS [Re: jcm4620]
#26450837 - 01/24/20 09:22 PM (4 years, 5 months ago) |
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Cloned this late flush Redboy cluster. Have a gallon of it colonizing and some clone test cakes ready to birth.

0228 I made lc and started some pf cakes, those colonized and I slurried them to a batch of wheat berry jars. Took some of those jars and spawned then to coir. One tray is normal sub depth, one tray is super deep sub depth. Both went in the FC a few days ago. They seem to be clean and lively so we'll see how these go.
The rest of the jars will be g2g or slurried to more wheat berry this weekend for mono spawn.

Edited by spiritlands (02/28/20 05:23 PM)
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redditone
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looks stunning man!
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A.k.a
Stranger



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Well mine went quick, there’s a lot but they’re small, curious how flush 2 will look. Pulled about 260 wet off the one tub and another 78 off part of the other one.
 

Clones don’t come up sterile more often right?? I noticed quite a few had no spores this time, not sure if it’s from harvesting a little earlier than usual now that i have prints or what. Even a bunch that had torn the veil completely and opened up more were bare. Pretty nice actually.
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LAGM2020     
Edited by A.k.a (01/25/20 11:03 AM)
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jcm4620
Stranger


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Re: CLONE WARS [Re: A.k.a]
#26451668 - 01/25/20 11:07 AM (4 years, 5 months ago) |
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😃😃👍
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: CLONE WARS [Re: jcm4620]
#26453290 - 01/26/20 10:32 AM (4 years, 5 months ago) |
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Yo you guys think I should try to grab this pin from a dusty ass RW germ plate? Or is it fucked? I’ve never attempted to clone anything.. this would be my first 

January 26 2020 I carefully grabbed pins from germ plate in the sab (Still expecting it to be hella dirty)  January 31 2020 Here is what the plates looks like 5 day’s latter. Going to grab my first set of transfers tonight.
February 6 2020 One week latter this is what the transfers look like. (Will be taking t3’s tomorrow)
February 14 2020 The transfers are looking clean so far. Might have todo one more transfer before grain?
February 20 2020 Plates are look clean but not as uniform as I’d like (Taking more transfers tonight)
 Thinking back on it I should left the pin 1 plate.. it didn’t need to be transferred it could of went straight to grains after I grabbed it from the germ plate LAGM 2020 Journal
Edited by staytrippy420 (02/20/20 12:55 PM)
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
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Let's see what happens!!
--------------------
Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Hell yeah gotta start somewhere 
There’s two pins there I’ll try to grab them this afternoon when I’m in the sab
-------------------- Tek's I use LAGM2020
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A.k.a
Stranger



Registered: 10/27/19
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Definitely.
I just pulled a tiny tiny pin off a clone plate, my first one, and overnight it’s already covered in myc.
You might as well grab both of those before that mold gets bigger.
It’s weird how they react to mold, some the myc won’t go near but some it doesn’t seem to notice. I have this nasty green brown hairy mold on an aa+ germ plate and the myc just ran it right over didn’t even slow down.
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LAGM2020     
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: CLONE WARS [Re: A.k.a]
#26453332 - 01/26/20 11:08 AM (4 years, 5 months ago) |
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Yeww. Stoked right now.
What’s the procedure? Flame tweezers, cool down and put to a new plate?
-------------------- Tek's I use LAGM2020
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A.k.a
Stranger



Registered: 10/27/19
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That should work.
I just poked it with the scalpel though, was gonna tweezer it but mine are super thick and stay hot forever. Plus the pin was tiny.
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LAGM2020     
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: CLONE WARS [Re: A.k.a]
#26453396 - 01/26/20 11:46 AM (4 years, 5 months ago) |
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Ahh yes, idk how the gf would feel about me flaming her tweezers... I’ll stick with the scalpel
-------------------- Tek's I use LAGM2020
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YogiBear



Registered: 08/24/19
Posts: 845
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Quote:
staytrippy420 said: Yo you guys think I should try to grab this pin from a dusty ass RW germ plate? Or is it fucked? I’ve never attempted to clone anything.. this would be my first 

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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Super stoked.. probably going be a bitch to clean up but I could use the practice. 
Quote:

-------------------- Tek's I use LAGM2020
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YogiBear



Registered: 08/24/19
Posts: 845
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Quote:
staytrippy420 said: Super stoked.. probably going be a bitch to clean up but I could use the practice. 
Quote:

Congrats!!!
I'm waiting on my first pins from Rusty and PESA 🥰
Edited by YogiBear (01/27/20 03:39 AM)
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cronicr



Registered: 08/07/11
Posts: 61,478
Loc: Van Isle
Last seen: 2 hours, 26 minutes
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Re: CLONE WARS [Re: cronicr]
#26454873 - 01/27/20 09:56 AM (4 years, 4 months ago) |
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Quote:
cronicr said:
 I'm gonna clone this but not sure how I will proceed just yet being that it is 2 blobs with a few fruits growing on top of them lol
 picked this guy who weighed in at 102 grams wet
update, picked the fat slob from that weird cluster
-------------------- It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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A.k.a
Stranger



Registered: 10/27/19
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Re: CLONE WARS [Re: cronicr]
#26454879 - 01/27/20 09:59 AM (4 years, 4 months ago) |
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Damn that’s a fat mushroom.
My other box of the same clone finished, idk if I’ll keep it going the pinset was great but they’re so small. Both came in around 270 wet, I think the second flush might be the deciding factor. Although I still need to test these, if they’re potent that’d be nice, they’re so small they dry real fast and don’t take much room.
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LAGM2020     
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: CLONE WARS [Re: A.k.a]
#26462049 - 01/31/20 12:21 PM (4 years, 4 months ago) |
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Updated op & my journal. 
Quote:
January 31 2020 Here is what the plates looks like 5 day’s latter. Going to grab my first set of transfers tonight. (This other germ plate Is now pinning might grab another one) 

-------------------- Tek's I use LAGM2020
Edited by staytrippy420 (01/31/20 12:29 PM)
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YogiBear



Registered: 08/24/19
Posts: 845
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Re: CLONE WARS [Re: YogiBear] 1
#26464530 - 02/02/20 01:08 AM (4 years, 4 months ago) |
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Quote:
YogiBear said: I'm working on this UPSIDE DOWNER PE6 right now 😆
Doesn't look promising though as my first attempt at cloning.

I'm out of the game. My UpsideDowner PE6 didn't make it. Was to bad off and was just getting dirty transfers.
Bye bye little Upside Downer ... RIP
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Zachsonpub
Friend



Registered: 03/02/16
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Damn, I was interested in seeing what a clone of this would do.
Imagine a canopy stems
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A.k.a
Stranger



Registered: 10/27/19
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You’re not really supposed to clone a clone right?
Would that mean putting a pin from a clone plate to another plate wouldn’t be the best either??
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LAGM2020     
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: CLONE WARS [Re: A.k.a]
#26468106 - 02/04/20 08:53 AM (4 years, 4 months ago) |
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How are you suppose to narrow down genetics if you can’t clone a clone?
Far as I know, you want the pin off the plate because of it’s fast genetics.
But don’t take my word for it. This is my first time cloning mushrooms.
-------------------- Tek's I use LAGM2020
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spiritlands



Registered: 06/21/10
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I'm pretty sure you can expand Mycelium a lot before senescence. I was wondering something similar though. Like let's say you clone a cluster and regrow, then you clone the best cluster again. If you take the prints from those is that better than taking the print from one phenotype and regrowing looking for the same pheno?
I'm asking bc I haven't seen any red spores on my RDU trays yet but there have been some with what look like empty gills. Haven't tried printing any yet most are still maturing. This is Stone Suns grow and they're all red, I'm guessing bc they're clones. Are clone prints of a pheno better for stabilizing a trait than selecting over generations or is it the same?
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A.k.a
Stranger



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I was told to clone grow it out then take a print and grow that and clone again.
Not sure if that’s correct that’s why I’m wondering now that I’ve got clones growInf.
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LAGM2020     
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: CLONE WARS [Re: A.k.a]
#26472796 - 02/06/20 08:45 PM (4 years, 4 months ago) |
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Hmmm.. guess we will find out
Updated OP.
Quote:
February 6 2020 One week latter this is what the transfers look like. (Will be taking t3’s tomorrow)

-------------------- Tek's I use LAGM2020
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bw86
Doesn't play well with others


Registered: 11/12/06
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just stopin in to show off Azurs original boner cloner
Quote:
azur said:

I still have it banked.The fattest i ever got from the clone
 
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A.k.a
Stranger



Registered: 10/27/19
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Re: CLONE WARS [Re: bw86]
#26472966 - 02/06/20 09:58 PM (4 years, 4 months ago) |
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Wtf!
Do you know the weights?
That one looks like it would be a qp dry
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LAGM2020     
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: CLONE WARS [Re: A.k.a]
#26473014 - 02/06/20 10:24 PM (4 years, 4 months ago) |
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-------------------- Tek's I use LAGM2020
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Zachsonpub
Friend



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Re: CLONE WARS [Re: bw86]
#26473848 - 02/07/20 12:08 PM (4 years, 4 months ago) |
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Quote:
azur said:

I laugh every time I see this one he took to bed.
Would love to try a tub of that and Avery.
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Doyledozo
Humble student



Registered: 10/22/19
Posts: 156
Last seen: 1 year, 22 days
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My biggest fruit so far was this 46g sporeless chitwan. Tissue sample also gave me my prettiest agar plate so far. Looking forward to bringing it back.http:// http://
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A.k.a
Stranger



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Looks good.
Now that I’ve done a bunch of spore plates I’m appreciating how nice clone myc is right off the bat.
I’m getting ready to dump my first agar pin plate to grain.
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LAGM2020     
Edited by A.k.a (02/09/20 12:39 PM)
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: CLONE WARS [Re: A.k.a] 1
#26477914 - 02/10/20 02:52 AM (4 years, 4 months ago) |
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For sure. That was the first thing I noticed too, nice clean uniform growth from the jump.
I’m not far behind you, prepped some wheat and took a transfer from my RW pin. Won’t be long now.
-------------------- Tek's I use LAGM2020
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Up dated op. 
Quote:
February 14 2020 The transfers are looking clean so far. Might have todo one more transfer before grain?

-------------------- Tek's I use LAGM2020
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MushkingMulah360
Amateur Mycologist


Registered: 01/13/20
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Looking good...I’d say on more would do it.
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A.k.a
Stranger



Registered: 10/27/19
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So a while back one of my first grows was Burma that didn’t go great.
I took a clone, almost positive it was one of these, if not it was from one just like them.

They’re super slow and like to grow on the bottom for some reason, but here’s my first run of the clone with some surface clusters.
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LAGM2020     
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: CLONE WARS [Re: A.k.a]
#26486846 - 02/15/20 10:26 AM (4 years, 4 months ago) |
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Haha nice Ak! A full canopy of those things would be dope!
-------------------- Tek's I use LAGM2020
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A.k.a
Stranger



Registered: 10/27/19
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Lol right that would be funny. The one on the left is so weird the cap is like squared.
I’ve got two pe clones I just put into fruiting today after casing and consolidating for a while, super excited for those. One was super potent even for pe.
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LAGM2020     
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,904
Loc: Canada
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Re: CLONE WARS [Re: A.k.a]
#26487250 - 02/15/20 03:31 PM (4 years, 4 months ago) |
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Supporter account ran out today so I can't post anymore pics, update soon to come.
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cronicr



Registered: 08/07/11
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let me fix that for ya
-------------------- It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Zifozonke
Stranger


Registered: 03/24/19
Posts: 1,259
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Re: CLONE WARS [Re: cronicr]
#26489628 - 02/17/20 08:40 AM (4 years, 4 months ago) |
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Hey fellow cloners... Hope youre all having better success with your projects than me... Ive just updated my post...and not with great news at all ...battling heat and contams...but still in it..and next up it will be my RWs. (No problems hopefully)
Will roll the dice again on my PE and keep at it. I really think there may still be something good hiding in there and not just contams....
Cheers guys!!
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: CLONE WARS [Re: A.k.a]
#26490038 - 02/17/20 01:33 PM (4 years, 4 months ago) |
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Quote:
A.k.a said: So a while back one of my first grows was Burma that didn’t go great.
I took a clone, almost positive it was one of these, if not it was from one just like them.

They’re super slow and like to grow on the bottom for some reason, but here’s my first run of the clone with some surface clusters.

How's the potency on these? They look cool
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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A.k.a
Stranger



Registered: 10/27/19
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They’re in the dehydrator now so I’ll find out soon. I never tried the ones they came from but was told they were awesome. They took long enough, I think 18 days to pin.
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LAGM2020     
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: CLONE WARS [Re: A.k.a]
#26495132 - 02/20/20 12:51 PM (4 years, 4 months ago) |
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Update time! How’s everyone’s clones doing?
Quote:
February 20 2020 Plates are look clean but not as uniform as I’d like (Taking more transfers tonight)

Edit: Thinking back on it I should left the pin 1 plate.. it didn’t need to be transferred it could of went straight to grains after I grabbed it from the germ plate
-------------------- Tek's I use LAGM2020
Edited by staytrippy420 (02/20/20 01:12 PM)
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Just spawned a tub Licked up some new tubs today and trying out pastys EZ dialed method
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Zachsonpub
Friend



Registered: 03/02/16
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Quote:
ComebackKid said:

 I've been looking forward to trying these Hope they work out for ya.
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A.k.a
Stranger



Registered: 10/27/19
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I’ve been eyeing those also, although so far I’ve been having good results with unmodded. I think probly cuz rh is so low where I’m at it doesn’t take much.
So two of my three PE clones are knotting, super excited.
One is taken from the big cap dead center

And one is from the big one here which was potent even for PE and grew days before any other pins so I’ve got high hopes for it.

Forgot to update my last one, this is how the Burma clone turned out. Haven’t had pins everywhere like this since my first few runs lol.

Doesn’t look it but still put out 25 dry.
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LAGM2020     
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MLPismyOPSEC
That One Ponyfucker


Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester...
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Re: CLONE WARS [Re: A.k.a] 1
#26504730 - 02/26/20 11:44 AM (4 years, 4 months ago) |
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Finally updated my post! Have hardly had any time to lurk let alone actually post, shit. Going to drop the pics here for a quickie preview.
 
Edited by MLPismyOPSEC (02/26/20 11:44 AM)
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Zifozonke
Stranger


Registered: 03/24/19
Posts: 1,259
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A.k.a
Stranger



Registered: 10/27/19
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Loc: Gaming the system
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Are those ape uncased?
I gotta grab pics of my PE clones. I have two shoeboxes of each, the ones from the fat shroom in the little carton are looking awesome. Just a crazy amount of pins coming in.
The other ones I thought would be great since I cloned them from a cluster on a shoebox that put out 350 wet from one quart, but they’re both super grown into the casing and it’s looking like blob city. Depending how they go I might run another one and skip the consolidation and double the casing depth.
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LAGM2020     
Edited by A.k.a (02/26/20 01:29 PM)
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MLPismyOPSEC
That One Ponyfucker


Registered: 11/13/18
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Re: CLONE WARS [Re: A.k.a]
#26505472 - 02/26/20 08:19 PM (4 years, 4 months ago) |
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Yeah, uncased. Just a top layer of coir/verm. I have all the material to case them, but when it came to spawning day, i forgot about it and then said fuck it, a top layer is good!
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A.k.a
Stranger



Registered: 10/27/19
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Ok so here’s my PE clone that’s not overgrowing the casing.
At what point do you consider bottom watering? If all these pins keep growing it should easily be my biggest yield based on how the ms PE went.

Hopefully they’ve got the potency of the donor.
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LAGM2020     
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MLPismyOPSEC
That One Ponyfucker


Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester...
Last seen: 3 months, 8 days
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Re: CLONE WARS [Re: A.k.a]
#26506196 - 02/27/20 09:35 AM (4 years, 3 months ago) |
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Now would be a good time. I usually switch to bottom watering once the pins get about that size. Sometimes i still mist over them, too.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: CLONE WARS [Re: A.k.a]
#26506386 - 02/27/20 11:53 AM (4 years, 3 months ago) |
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Quote:
A.k.a said: Ok so here’s my PE clone that’s not overgrowing the casing.
At what point do you consider bottom watering? If all these pins keep growing it should easily be my biggest yield based on how the ms PE went.

Hopefully they’ve got the potency of the donor.
Damn that's a beauty pin set!
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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spiritlands



Registered: 06/21/10
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Loc:
Last seen: 6 months, 24 days
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First update on the Redboy cluster clone. Trying these out in the Martha. Will be expanding my spawn for some monos this weekend.

Update 0301

Edited by spiritlands (03/01/20 11:28 PM)
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A.k.a
Stranger



Registered: 10/27/19
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So my first tub of PE clone didn’t do as well as I hoped based on the pinset, but decent. Plus there’s a couple that look identical to the original. One is at the very bottom of the pic with the open cap, same shape super thick stem so hopefully it’s also got the potency.
. The original. 
The second tub of this clone looks like it’ll be more filled in.
Edit- I harvested the first shoebox and although it didn’t look very big it was just shy of 400g. The second shoebox of the same clone is filling in more, it would be so sick to break 500 off a one quart first flush.
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LAGM2020     
Edited by A.k.a (03/04/20 12:40 PM)
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Zifozonke
Stranger


Registered: 03/24/19
Posts: 1,259
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Re: CLONE WARS [Re: A.k.a]
#26525445 - 03/09/20 12:49 PM (4 years, 3 months ago) |
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*Updated*my grow Not the greatest results-but a result nontheless...first time Ive ever participated in something like this-maybe the last!!-seems I have better luck when Im not under pressure
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A.k.a
Stranger



Registered: 10/27/19
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Oh me too.
Both came to 450-485.
Definitely doing a bigger tub of them, especially since I can’t wait to see how the one does now that I know it needs extra casing.

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LAGM2020     
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: CLONE WARS [Re: A.k.a]
#26525875 - 03/09/20 05:52 PM (4 years, 3 months ago) |
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Damn aka. You been killing it 

-------------------- Tek's I use LAGM2020
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A.k.a
Stranger



Registered: 10/27/19
Posts: 17,177
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Thanks man, I’d run a few clones of maz gt and Burma before this and was like eh...clones are ok but not much better than ms so far.
Then I got these two, what a difference. It also helps I’m finally getting a handle on conditions, zero side pins on about half my recent tubs. lol I was so happy the first time I was harvesting and realized there weren’t any under the liner.
In clone news, I dropped a plate pin of toc to grain today which is a first, and also have a ton of good growth on my mega cluster clone from my toc shoebox. I really want to transfer a tiny wedge and grain the rest but I feel weird dumping an old tissue sample into a jar for some reason.
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LAGM2020     
Edited by A.k.a (03/09/20 06:21 PM)
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cronicr



Registered: 08/07/11
Posts: 61,478
Loc: Van Isle
Last seen: 2 hours, 26 minutes
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Re: CLONE WARS [Re: A.k.a]
#26548967 - 03/21/20 01:45 PM (4 years, 3 months ago) |
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after I made my first transfer I had to put cult aside for awhile and now with everything happening I'm not sure I'll be able to move on anytime soon, shits hard to come by now
-------------------- It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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spiritlands



Registered: 06/21/10
Posts: 1,616
Loc:
Last seen: 6 months, 24 days
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Re: CLONE WARS [Re: cronicr]
#26550118 - 03/22/20 02:40 AM (4 years, 3 months ago) |
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Well, here's an update on the Redboy cluster clone. This tray has some kind of contam that was eating away the bases of some of the mushrooms. I finally started using hydrated lime in my casing and it seemed to keep the contam at bay. Went ahead and let these mature a little but the Martha is getting a deep clean tomorrow and this tray is going in the trash. I do like how many clusters I got so I'll have to dig out my backup lc from this culture.
 Had hoped for better but at least I'll have something to munch.
I feel you on the supplies Cron. Luckily I picked up a bag of wbs and a bail of chopped straw for my pans. Still have several bags of manure and a couple big bricks of coir. I'll be ok for a while but looks like imma be getting comfortable using straw for everything.
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One of Us
Stranger



Registered: 03/12/12
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Can I join in? I have 2 melmac candidates.
1. big guy (the gentleman in the middle of the cluster pics)
  
2. Crinkle cap
  
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Zifozonke
Stranger


Registered: 03/24/19
Posts: 1,259
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Nice cluster there One of Us. Those are picture perfect!!
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alaskappalachian
Entitiologist


Registered: 10/22/19
Posts: 1,963
Loc: The 49th Dimension
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Disappointing pinset on this PF classsic clone. 2nd time I've run it and same weirdo caps, but crazy potent (most of the Classics I've run have been atypically potent) and first flush still yielded 321g (solid stipes). Prints of largest caps going to agar tonight and the search continues...
-------------------- THE 49TH MYCOJOURNAL: EXOTICS, AURORAS, & ENTITIES "It is all one vast awakened thing. I call it the golden eternity. It is perfect." -Kerouac
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A.k.a
Stranger



Registered: 10/27/19
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Quote:
One of Us said: Can I join in? I have 2 melmac candidates.
1. big guy (the gentleman in the middle of the cluster pics)
  
2. Crinkle cap
   
Are your stems solid?? I just did my first melmak and they came out like bigger than normal regular cubes, super hollow though.
Alaska that’s a good weight for the pinset, one quart??
Classics always look so rough compared to other kinds. Feral cubes.
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LAGM2020     
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alaskappalachian
Entitiologist


Registered: 10/22/19
Posts: 1,963
Loc: The 49th Dimension
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Re: CLONE WARS [Re: A.k.a]
#26561076 - 03/27/20 12:10 PM (4 years, 3 months ago) |
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Quote:
Alaska that’s a good weight for the pinset, one quart??
Classics always look so rough compared to other kinds. Feral cubes.
Thanks bro- it was <1 1/2pts. I almost never use a full quart on a shoebox. Got almost the exact same weight with last box I ran of this clone. Nice, solid stipes and crazy potent for a "regular" cube, but fugly af.
Quote:
A.k.a said: Oh me too.
Both came to 450-485.
Definitely doing a bigger tub of them, especially since I can’t wait to see how the one does now that I know it needs extra casing.
 
Might I add... fuckin aye.
-------------------- THE 49TH MYCOJOURNAL: EXOTICS, AURORAS, & ENTITIES "It is all one vast awakened thing. I call it the golden eternity. It is perfect." -Kerouac
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Buddaking
Genetics



Registered: 12/18/09
Posts: 1,775
Loc: No Mans Land
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Did not get a before. Not bummed but scraping this one based on size... Smaller than I was hoping.
--------------------
 AMU Q & A
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A.k.a
Stranger



Registered: 10/27/19
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Damn that’s a filled in pinset thoUgh, especially with no side pins in a bag. Kinda makes me want to try those out.
If they’re super potent I’d love that culture even with its skinny dicks.
Does anybody ever take multiple transfers from the tissue plate and grow them out individually?
The extreme difference in my clone between t1 and t3 makes me think the other plates I have from the same original tissue plate are probably all very different if I were to grow them.
Or even different tissue samples from the same mushroom would be interesting.
So many experiments to do.
Edited by A.k.a (03/27/20 04:26 PM)
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One of Us
Stranger



Registered: 03/12/12
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Re: CLONE WARS [Re: A.k.a]
#26561611 - 03/27/20 05:11 PM (4 years, 3 months ago) |
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Quote:
A.k.a said:
Quote:
One of Us said: Can I join in? I have 2 melmac candidates.
1. big guy (the gentleman in the middle of the cluster pics)
  
2. Crinkle cap
   
Are your stems solid?? I just did my first melmak and they came out like bigger than normal regular cubes, super hollow though.
Those two were pretty solid, but I picked some today that were definitely super hollow like you say
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Hobbit GDF
Deadhead



Registered: 02/14/19
Posts: 3,540
Loc: Terrapin station
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Ok so I'm working on a few and font have pics of some. But I do of my "king kong" APE clone. First time me fruiting this clone. I also have another ape clone and a swab of one fruiting. Idk if the swab shoebox will make apes. They look like it so far.
Here's "KingKong" ...


And here is the first shoebox of the KK

Nice fat pins. I just need to get them clustered. Maybe cross with a more clustering culture of ape?
I'm working on Melmak 
Pe6. This is clone tub 
Aa+ . 
Golden halo. 
Tailfeathers & white TF (no parent pic) LPEU (no pic)(I'm excited about this one), the fruit were huge and the mono flushed amazingly. Costa rican (clone sucked inch tall). 
mystery variety -clone tub, that turned out well. 
Well that's what I have to show anyhow.
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A.k.a
Stranger



Registered: 10/27/19
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lol that kk tub looks like an alien nest with all the myc tentacles and fat egg pins.
Looks like they’ll be nice and beefy.
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LAGM2020     
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peanotter
Stranger
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Re: CLONE WARS [Re: A.k.a]
#26562564 - 03/28/20 05:53 AM (4 years, 3 months ago) |
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So if we're not supposed to clone a clone (per RR), how would we narrow down the specific phenotype we're after?
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Hobbit GDF
Deadhead



Registered: 02/14/19
Posts: 3,540
Loc: Terrapin station
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Re: CLONE WARS [Re: A.k.a]
#26562672 - 03/28/20 08:26 AM (4 years, 3 months ago) |
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Quote:
A.k.a said: lol that kk tub looks like an alien nest with all the myc tentacles and fat egg pins.
Looks like they’ll be nice and beefy.
I wish I could get my old AA+ genetics back. They in my sig. They were the same way. Got really fat bottom. Pins looked like them ape pins.
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A.k.a
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Quote:
peanotter said: So if we're not supposed to clone a clone (per RR), how would we narrow down the specific phenotype we're after?
I’ve been trying to get a solid answer.
So far I think you clone the one you like, grow it out and take a print from one that looks similar, grow that and it should have a good chance of more of that pheno and then you clone and repeat.
However multiple people have said they clone a clone of a clone of a clone no problem.
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Buddaking
Genetics



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Re: CLONE WARS [Re: A.k.a] 1
#26562824 - 03/28/20 09:46 AM (4 years, 3 months ago) |
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When I get a good clone I just work out each sector till I Isolate the genitic trait I desire. Ya end up with a lot of petri's so labeling & space to slow em down in cold storage is important. Once I find the "one" I go back & clear out the fridge of unwanted & make atleast 3 slants to fall back on.
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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I lost / abandoned my original project but these both came from an albino cambo streak plate. One really fat pin and one really long pin. Going to run them side by side and see what’s good.

I’ve gotta get my cold storage going.. Juggling cultures around is a bitch.
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Hobbit GDF
Deadhead



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Quote:
Hobbit GDF said: Ok so I'm working on a few and font have pics of some. But I do of my "king kong" APE clone. First time me fruiting this clone. I also have another ape clone and a swab of one fruiting. Idk if the swab shoebox will make apes. They look like it so far.
Here's "KingKong" ...


And here is the first shoebox of the KK

Nice fat pins. I just need to get them clustered. Maybe cross with a more clustering culture of ape?
I'm working on Melmak 
Pe6. This is clone tub 
Aa+ . 
Golden halo. 
Tailfeathers & white TF (no parent pic) LPEU (no pic)(I'm excited about this one), the fruit were huge and the mono flushed amazingly. Costa rican (clone sucked inch tall). 
mystery variety -clone tub, that turned out well. 
Well that's what I have to show anyhow.
Ok harvested the ape clone sb. Wet weight was only 188g. Sharpie for size scale.
 None was as big as parent. I cloned it again. How many times can I do that? I will just find out. I'll restart from next set spores.
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A.k.a
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Keeping this thing going.
So for lagm I did my first toc grows.
Each shoebox had a great cluster that I cloned.

Both were spawned 7 and 4 days ago.
The older one was knotting on day 6 and the other one I ran out of coir and didn’t add a top layer til the end of the second day. 16 hours after spawning it was already popping rhizo off the exposed grains. Added the coir and today, two days later, it’s to the surface on about 70%.
High hopes for both these guys.
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trubblesome
Stranger



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Re: CLONE WARS [Re: A.k.a]
#26599271 - 04/13/20 11:29 PM (4 years, 2 months ago) |
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Nice work Hobbit and Aka
I'll finally be spawning my "Nip B+" tomorrow. Shoebox. Will print, grow and hopefully get a larger nip fruit from that batch to clone and so on until I have a reliably nip cap'd culture.
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woofwoof
such mushrooms!



Registered: 01/04/19
Posts: 1,127
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What traits is everybody after for cloning? Large fruit? Potency? Canopies? Canopies are surface condition dependent or can clones recreate canopies?
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A.k.a
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Potency speed and yield for me.
So these are the clones spawned on the 6th and 9th, the younger one top layered on the 11th.

More advanced one is the cluster still on the sub in the earlier pic.
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trubblesome
Stranger



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Re: CLONE WARS [Re: A.k.a]
#26602976 - 04/15/20 01:18 PM (4 years, 2 months ago) |
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Okay, spawned Nip B+ and Big GT today. These are the deepest shoeboxes I've ever done. spawned 1:2.5 with a half quart casing layer. forgot to get pics of the jars pre-spawn.
Nip B+ original:

The Clone boxes:

I hit the jackpot on clusters with this PESA tub. Unfortunately I also hit the jackpot on mold. I caught it before it went green but I cut this whole section out - the casing wasn't colonizing and the tub began to get a mildew smell. I cut it out and put it in a bowl and on close examination, what appeared to have been knots was the beginning of some kind of mold growing. I put the bowl outside and the next day it was green, maybe trich
Anyway I think I bought myself enough time to get a decent harvest as well as get a couple clones from these clusters. I think the clusters came from how I chose the mycelium from the plates but I'll leave that for another post. I will probably do something similar with the clones I gather from this tub...
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Camera93
We got dicks like Jesus



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Re: CLONE WARS [Re: A.k.a]
#26602989 - 04/15/20 01:21 PM (4 years, 2 months ago) |
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Quote:
A.k.a said: Potency speed and yield for me.
So these are the clones spawned on the 6th and 9th, the younger one top layered on the 11th.

More advanced one is the cluster still on the sub in the earlier pic.
How are you testing for potency? I keep getting ready to ask about potency on TOC but I know I'll get "the cube is a cube/have to clone and isolate for consistent potency/ it's all multi-spore and random hit&miss w/o isolating"
but if someone cares to toss their anecdotal evidence into the mix for me  (in comparison to rustywhytes)**
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
Edited by Camera93 (04/15/20 01:22 PM)
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woofwoof
such mushrooms!



Registered: 01/04/19
Posts: 1,127
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I am hoping to do a clone of my RW that are about to pin. I suppose cloning for potency is gunna be hard because there is no way to know until you clone them then harvest and dose.
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A.k.a
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lol Idk either really.
I guess yeah wait til you find one that does well and eat some hoping it’s acceptable.
I’ve heard of people taking a tissue sample then eating the mushroom also but I’m not sure how reliable it is.
My main question is with ms grows you could pick two random shrooms and one could be five times stronger than the other. So is a clone grow the same just with a smaller range in potency?
It doesn’t seem like they would all be the same strength unless the culture was super narrowed down.
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trubblesome
Stranger



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Re: CLONE WARS [Re: A.k.a] 1
#26606172 - 04/16/20 04:38 PM (4 years, 2 months ago) |
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Okay here we go. Woke up to this

Picked 8 of the largest clustered shrooms, and then one fat side pin for cloning. side pin turned out to be in a cluster too. So here begin my PESA culture projects.

These are the mushrooms I picked. Labeled in rough order of size 1-7, corresponding with the labels on the dishes. I didn't think to arrange them and label by size until after I was all up in the SAB. When I chose mycelium to inoculate the master jar with for this grow, I took from a plate that held three different transfers from the 3 fastest rhizo growths on the previous plate. when I inoculated the master jar, I took a wedge from each intersection of these growths, in the place where I could see that the mycelium was compatible and clearly linking. I'm hoping that this selection method and the genetics isolated together had something to do with the clustering. So I'm going to do something similar with the clones. I took clones by taking a small prism shaped piece out of each mushroom's cap tissue:

I make my slices in the order and direction indicated in the photo - Slice 1 and 2 are done first because it's easier to cut across the grain when you do them first. Slice 3 and 4 are angled toward each other so that the resulting piece of tissue is kind of a prism shape and is removed easily if not when I make my last slice, then by simply poking it with the tip of the scalpel blade. To get to this point, I simply grab the mushroom at the base of the stem and rip longways. It usually is happy to break right down the middle. Halfway through I adjust the way I hold it so that the cap is upward, this way nothing from my hands or on the outside of the stem can fall in to the open middle portion of the cap once I split it open. keep the cap elevated until you make your cut, at which point only the scalpel should move above it. I figure this way increases the odds of not carrying stray cube spores or anything wack like mold and bacteria with it.

I placed the cuttings on their own halves of split petri dishes. I think I may isolate "Fat Boy" on its own and do a solo shoebox with it to prove its genetics. The rest are going to be transferred to combo dishes to find matching pairs once I get something clean from each.
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A.k.a
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Coming along nicely.
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trubblesome
Stranger



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Re: CLONE WARS [Re: A.k.a]
#26624495 - 04/24/20 11:18 AM (4 years, 2 months ago) |
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clone dishes looking good, clean as fuck, which is a relief since I had a bad SAB session last week and had to make a ton of transfers last night to get away from shit on dishes. I also realized I could just drop all these in to their own jars or in to one bag and mix the genetics at spawn or inoculation rather than waste time dicking around with enough plates to have every combo covered. so I'll probably do that. I'm gonna drop fat boy in its own grain jar at least.

The clone shoeboxes are about to pop. guessing I'll have a pinset going monday. hopefully the nip was genetic. I don't even remember what "Big GT" looked like, so hopefully it's just fucking big and has a nice cap for printing.
Nip B+:

Big GT:
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A.k.a
Stranger



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Oh yeah those are ready for pins.
Forgot to update here but my toc clones were eh. One decent at like 300 I think and the other like 180.

They came from such good clusters too.
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Wolfenstein
Learning & Yearning


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Re: CLONE WARS [Re: A.k.a]
#26624723 - 04/24/20 01:03 PM (4 years, 2 months ago) |
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Ah shit buddy you're getting me all sorts of excited
-------------------- Anything and everything I say, write, and/or imply is purely false and is only done so with the intent to enhance my acting career. Molon Labe As above, so below
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trubblesome
Stranger



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I'd been having some trouble with FAE and humidity in the room my shroomies are in, started to get some side pins on the Nip B+, so I said fuck it and plopped both shoeboxes in the fruiting tent for my edibles since I don't have any fruiting right now and i've never done it with the actives.

Only problem is once I kicked the humidifier back on the temp dropped 10 degrees to around 60 and I didn't realize it til late at night. made some tweaks and no change the next morning. more tweaks and no change somehow. finally just rerouted the air flow with the plant tent and got it right today. but they had a couple days there in the low 60s that kinda stalled 'em out a bit. I worried that I fucked up the clone somehow too as they weren't very nipply but the nip came out a bit once the veil broke and the cap started to broaden:


Still not as nipply as the original. they are my favorite size though, 4 inches or so, fatter around than a sharpie, dense fruits. they'll probably look good dried. they're not the nipple clones of my dreams though, so back to the drawing board there. Plucked the ones with broken veils and left the rest to develop in better conditions.
so far "big GT" is just a kind of solid, meaty shroom that's about 4" high, but how much of that is from the temp drop who knows. We'll see how the rest of the developing shrooms do now that things are back to 70.
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Wolfenstein
Learning & Yearning


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He'll yeah man!
-------------------- Anything and everything I say, write, and/or imply is purely false and is only done so with the intent to enhance my acting career. Molon Labe As above, so below
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DeckardCain
Stay a while and listen



Registered: 09/22/12
Posts: 236
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The tallest guy you can see
 Then cloned >>  And voila >>
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Hobbit GDF
Deadhead



Registered: 02/14/19
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Here are 2 clones from same tub. The variety is tailfeathers. Here is the first tub I had of them.

As you see , there are white ones. I cloned some of both phenotypes. The ones I have left are tailfeathers3 and white tailfeathers 1 (wtf1). This is the first time I've spawned them. I really like how they look.
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A.k.a
Stranger



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Those have awesome caps, I’m into the spots.
So with tail feathers is it normal to get white mixed in like the first tub or is that unusual?
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Hobbit GDF
Deadhead



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Posts: 3,540
Loc: Terrapin station
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Re: CLONE WARS [Re: A.k.a]
#26651368 - 05/05/20 06:10 PM (4 years, 1 month ago) |
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This was 3rd generation from print. This is the first one that done it from what I was told about the other two.
One of the parents of TF is AA+ so I understand where it's coming from. Weird how instead of white it turned out yellow on the clone.
I have fruited TF ms again after that from same culture plates. Maybe 4 transfers away from swab. They fruited normal brown/ tan that cubes make
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A.k.a
Stranger



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Oh that’s awesome you were able to isolate the lighter ones then.
Man aa+ is a stud mushroom. Seems like it’s gotten into half the varieties at some point.
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Wolfenstein
Learning & Yearning


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Re: CLONE WARS [Re: A.k.a]
#26651451 - 05/05/20 06:42 PM (4 years, 1 month ago) |
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Ha! Sorry that was just really well said. Interesting to think about it from an biological concept theory/evolutionary standpoint.
I'm super uneducated in regards to (a lot of things) what varieties came from where and who and when. It'd be sweet to make up a phylogenetic tree for all these bad boys.
-------------------- Anything and everything I say, write, and/or imply is purely false and is only done so with the intent to enhance my acting career. Molon Labe As above, so below
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trubblesome
Stranger



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Nip B+ and "big" GT update: ~200 g wet each. I harvested them both together because they ended up looking quite similar anyway and fuck it. for the hell of it i decided to let them go for a second flush but Nip B+ blue/green molded on me. this shit is everywhere in here it's driving me nuts.
despite the blue/green mold making it's presence felt all over my grows, the PESA plates looked fine so I just dropped a wedge from each to grain, no transfers ¯\_(ツ)_/¯ nothing matters anymore. we'll see if enough of these make it for a tub:
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Hobbit GDF
Deadhead



Registered: 02/14/19
Posts: 3,540
Loc: Terrapin station
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Here are my LPEU
Ms

I took 3 clones. This is how 1 looks

This is 2. Little behind 1.

Number 3 is still being worked on.
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A.k.a
Stranger



Registered: 10/27/19
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Damn those are nice, I gotta find my print.
Did the clone weigh more than the ms?
Looks like it had way bigger stems.
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Hobbit GDF
Deadhead



Registered: 02/14/19
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Quote:
Hobbit GDF said: Here are my LPEU
Ms

I took 3 clones. This is how 1 looks

This is 2. Little behind 1.

Number 3 is still being worked on.
Update. Here is number 2
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A.k.a
Stranger



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That’s a nice cluster.
Are those boot boxes?
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Hobbit GDF
Deadhead



Registered: 02/14/19
Posts: 3,540
Loc: Terrapin station
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Re: CLONE WARS [Re: A.k.a]
#26683751 - 05/20/20 04:49 PM (4 years, 1 month ago) |
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Ms- 16qt box
Clone 1- shoebox
Clone 2- shoebox
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Hobbit GDF
Deadhead



Registered: 02/14/19
Posts: 3,540
Loc: Terrapin station
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Quote:
Hobbit GDF said:
Quote:
Hobbit GDF said: Here are my LPEU
Ms

I took 3 clones. This is how 1 looks

This is 2. Little behind 1.

Number 3 is still being worked on.
Update. Here is number 2

Ok so shoebox lpeu clone 2 dry weight is 30g. Very fucking happy with clone 2. Produced more than one
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trubblesome
Stranger



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alright alright time to update the clone wars
for one thing, I decided to revive the "big GT" - those fruits were just so cute and perfect, each one was .5 grams, and the soft blue of the bruising was bizarrely appetizing. took 2 G's and had a very pleasant time so decided it's a keeper. should have that on grain next week.
but I spawned the PESA clones after about a month + in jars... tub yielded 100g dry but I had to leave town before I could let it fully finish:


unfortunately over the weekend some mold popped up so it's gone now. buried in the backyard 
"fat boy" got its own little tub. it turned out fine, though I forgot to take a pic of the final result. looks like regular cubes ¯\_(ツ)_/¯
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A.k.a
Stranger



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Totally forgot about this thread.
Had an interesting one recently. Ran Melmak spores and got normal looking cubes. Clones the giant one in the picture. Grew it out and got these big PE pheno.

Also cloned this big late flush pe6.

Surface is almost solid knots, kinda weird.

Some pins showed up on the edges first and just finished, and now the whole thing is starting to pin. I’m out of picture space but the ones I pulled already were super dense and spongey like PE.
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ReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 2,022
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Re: CLONE WARS [Re: A.k.a]
#26809488 - 07/07/20 05:09 PM (3 years, 11 months ago) |
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Too late to join the party?
I took my first clones from this cluster of PE from a mss.
 
And here is the first flush from that clone.

Edit to add pic:
 I think this pin cluster from the second flush look just like their dad.
-------------------- Stoned Gummys | BRF Pucks | Primo Prepour Plates | Easy LI 4 Preserv & Propo"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
Edited by ReverendMyc (07/08/20 03:00 PM)
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A.k.a
Stranger



Registered: 10/27/19
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Nice. That cluster is amazing, I probably would’ve ended up with way too many clones to run from that.
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: CLONE WARS [Re: A.k.a]
#26901100 - 08/26/20 06:13 PM (3 years, 9 months ago) |
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Damn. Y’all been busy.
-------------------- Tek's I use LAGM2020
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